Addressing the metabolic activation potential of new leads in drug discovery: A case study using ion trap mass spectrometry and tritium labeling techniques

Journal of Mass Spectrometry

Volumn 38, Issue 2, 2003, Pages 211-221

  Samuel, Koppara ^a   Yin, Wenji ^a   Stearns, Ralph A ^a   Tang, Yui S ^a   Chaudhary, Ashok G ^a   Jewell, James P ^a   Lanza Jr , Thomas ^a   Lin, Linus S ^a   Hagmann, William K ^a   Evans, David C ^a   Kumar, Sanjeev ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Bioactivation; Covalent binding; Glutathione conjugates; Ion trap mass spectrometry; Microsomes; Reactive metabolites; Tritium labeling

Indexed keywords

IONS; ITERATIVE METHODS; LIQUID CHROMATOGRAPHY; MACROMOLECULES; MASS SPECTROMETRY; METABOLITES; RADIOACTIVITY;

ELECTROPHILIC REACTIVE METABOLITES;

DRUG PRODUCTS;

DRUG METABOLITE; GLUTATHIONE; NEW DRUG; THIOL; TRITIUM;

ANIMAL CELL; ARTICLE; CHEMICAL MODIFICATION; CONTROLLED STUDY; DRUG BINDING; DRUG IDENTIFICATION; DRUG METABOLISM; DRUG SAFETY; DRUG STRUCTURE; DRUG SYNTHESIS; FEASIBILITY STUDY; GLUTATHIONE METABOLISM; HUMAN; HUMAN CELL; IN VITRO STUDY; INCUBATION TIME; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; LIVER MICROSOME; MACROMOLECULE; MASS SPECTROMETRY; METABOLIC ACTIVATION; NONHUMAN; ONLINE ANALYSIS; PHARMACOKINETICS; PRIORITY JOURNAL; RADIOACTIVITY;

ANIMALS; BIOTRANSFORMATION; DRUGS, INVESTIGATIONAL; GLUTATHIONE; HUMANS; MALE; MICROSOMES, LIVER; RATS; RATS, SPRAGUE-DAWLEY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; STRUCTURE-ACTIVITY RELATIONSHIP; TRITIUM;

TRITIUM;

EID: 0037297445     PISSN: 10765174     EISSN: None     Source Type: Journal    
DOI: 10.1002/jms.434     Document Type: Article

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