RecFOR function is required for DNA repair and recombination in a RecA loading-deficient recB mutant of Escherichia coli

Genetics

Volumn 163, Issue 2, 2003, Pages 485-494

  Ivančić Baće, Ivana ^b   Peharec, Petra ^a   Moslavac, Sunčana ^a   Škrobot, Nikolina ^a   Salaj Šmic, Erika ^a   Brčić Kostić, Krunoslav ^a  

^a RUDER BO KOVI INSTITUTE   (Croatia)

^b UNIVERSITY OF ZAGREB   (Croatia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL ENZYME; BACTERIAL PROTEIN; EXONUCLEASE; HELICASE; RECA PROTEIN; RECFOR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; ASSAY; BACTERIAL GENE; BACTERIUM MUTANT; COMPARATIVE STUDY; CONTROLLED STUDY; DNA RECOMBINATION; DNA REPAIR; ENZYME ACTIVITY; ESCHERICHIA COLI; GAMMA IRRADIATION; GENE FUNCTION; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; RECB GENE; RECOMBINANT GENE;

DNA REPAIR; DNA, SINGLE-STRANDED; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; EXODEOXYRIBONUCLEASE V; EXODEOXYRIBONUCLEASES; GAMMA RAYS; MUTATION; REC A RECOMBINASES; RECOMBINATION, GENETIC; ULTRAVIOLET RAYS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0037295442     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (54)

1. AL-DEIB, A. A., A. A. MAHDI and R. G. LLOYD, 1996 Modulation of recombination and DNA repair by the RecG and PriA helicases of Escherichia coli K-12. J. Bacteriol. 178: 6782-6789.
2. AMUNDSEN, S. K., A. F. TAYLOR, A. M. CHAUDHURY and G. R. SMITH, 1986 recd: the gene for an essential third subunit of exonuclease V. Proc. Natl. Acad. Sci. USA 83: 5558-5562.
3. AMUNDSEN, S. K., A. M. NEIMAN, S. M. THIBODEAUX and G. R. SMITH, 1990 Genetic dissection of the biochemical activities of RecBCD enzyme. Genetics 126: 25-40.
4. AMUNDSEN, S. K., A. F. TAYLOR and G. R. SMITH, 2000 The RecD subunit of the Escherichia coli RecBCD enzyme inhibits RecA loading, homologous recombination, and DNA repair. Proc. Natl. Acad. Sci. USA 97: 7399-7404.
5. ANDERSON, D. G., and S. C. KOWALCZYKOWSKI, 1997a The recombination hotspot X is a regulatory element that switches the polarity of DNA degradation by the RecBCD enzyme. Genes Dev. 11: 571-581.
6. ANDERSON, D. G., and S. C. KOWALCZYKOWSKI, 1997b The translocation RecBCD enzyme stimulates recombination by directing RecA protein onto ssDNA in a x-regulated manner. Cell 90: 77-86.
7. D1080A eliminates RecA protein loading but not chi recognition by RecBCD enzyme. J. Biol. Chem. 274: 27139-27144.
8. ARNOLD, D.A., and S. C. KOWALCZYKOWSKI, 2000 Facilitated loading of RecA protein is essential to recombination by RecBCD enzyme. J. Biol. Chem. 275: 12261-12265.
9. BACHMANN, B.J., 1972 Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol. Rev. 36: 525-557.
10. BOEHMER, P. E., and P. T. EMMERSON, 1991 Escherichia coli RecBCD enzyme: inducible overproduction and reconstitution of the ATP-dependent deoxyribonuclease from purified subunits. Gene 102: 1-6.
11. BORK, J. M. M. M. COX and R. B. INMAN, 2001 The RecOR proteins modulate RecA protein function at 5′ ends of single-stranded DNA. EMBO J. 20: 7313-7322.
12. CHAUDHURY, A.M., and G.R. SMITH, 1984 A new class of Escherichia coli recBC mutants: implications for the role of RecBC enzyme in homologous recombination. Proc. Natl. Acad. Sci. USA 81: 7850-7854.
13. CHURCHILL, J.J., D. G. ANDERSON and S. C. KOWALCZYKOWSKI, 1999 The RecBC enzyme loads RecA protein onto ssDNA asymmetrically and independent of χ, resulting in constitutive recombination activation. Genes Dev. 13: 901-911.
14. CLARK, A.J., and S.J. SANDLER, 1994 Homologous recombination: the pieces begin to fall into place. Crit. Rev. Microbiol. 20: 125-142.
15. CONNELLY, J. C., E. S. DE LEAU, E. A. OKELY and D. R. F. LEACH, 1997 Overexpression, purification, and characterization of the SbcCD protein from Escherichia coli. J. Biol. Chem. 272: 19819-19826.
16. CORRETTE-BENNETT, S. E., and S. T. LOVETT, 1995 Enhancement of RecA strand-transfer activity by the RecJ exonuclease of Escherichia coli. J. Biol. Chem. 270: 6881-6885.
17. CROMIE, G. A., and D. R. LEACH, 2001 Recombinational repair of chromosomal DNA double-strand breaks generated by a restriction endonuclease. Mol. Microbiol. 41: 873-883.
18. CROMIE, G. A., J. C. CONNELLY and D. R. F. LEACH, 2001 Recombination at double-strand breaks and DNA ends: conserved mechanisms from phage to humans. Mol. Cell 8: 1163-1174.
19. DIXON, D.A., and S. C. KOWALCZYKOWSKI, 1993 The recombination hotspot χ is a regulatory sequence that acts by attenuating the nuclease activity of the E. coli RecBCD enzyme. Cell 73: 87-96.
20. GANESAN, A. K., and P. C. SEAWELL, 1975 The effect of lexA and recF mutations on post-replication repair and DNA synthesis in Escherichia coli K-12. Mol. Gen. Genet. 141: 189-205.
21. GIBSON, F. P., D. R. F. LEACH and R. G. LLOYD, 1992 Identification of sbcD mutations as cosuppressors of recBC that allow propagation of DNA palindromes in Escherichia coli K-12. J. Bacteriol. 174: 1222-1228.
22. HARMON, F. G., and S. C. KOWALCZYKOWSKI, 1998 RecQ helicase, in concert with RecA and SSB proteins, initiates and disrupts DNA recombination. Genes Dev. 12: 1134-1144.
23. HORH, Z. I., and A. J. CLARK, 1973 Genetic analysis of the RecF pathway of genetic recombination in Escherichia coli K12: isolation and characterization of mutants. J. Mol. Biol. 80: 327-344.
24. JOCKOVICH, M.E., and R.S. MYERS, 2001 Nuclease activity is essential for RecBCD recombination in Escherichia coli. Mol. Microbiol. 41: 949-962.
25. KOWALCZYKOWSKI, S. C., 2000 Initiation of genetic recombination and recombination-dependent replication. Trends Biochem. Sci. 25: 156-165.
26. KOWALCZYKOWSKI, S. C., D. A. DIXON, A. K. EGGLESTON, S. D. LAUDER and W. M. REHRAUER, 1994 Biochemistry of homologous recombination in Escherichia coli. Microbiol. Rev. 58: 401-465.
27. KUSHNER, S. R., H. NAGAISHI, A. TEMPLIN and A.J. CLARK, 1971 Genetic recombination in Escherichia coli: the role of exonuclease I. Proc. Natl. Acad. Sci. USA 68: 824-827.
28. KUZMINOV, A., 1999 Recombinational repair of DNA damage in Escherichia coli and bacteriophage λ. Microbiol. Mol. Biol. Rev. 63: 751-813.
29. LEHMAN, I. R., and R. NUSSBAUM, 1964 The deoxyribonuclease of Escherichia coli. V. On the specificity of exonuclease I (phosphodiesterase). J. Biol. Chem. 239: 2628-2636.
30. LLOYD, R. G., and C. BUCKMAN, 1985 Identification and genetic analysis of sbcC mutations in commonly used recBC sbcB strains of Escherichia coli K-12. J. Bacteriol. 164: 836-844.
31. LLOYD, R. G., M. C. PORTON and C. BUCKMAN, 1988 Effect of recF, recJ, recN, recO and ruv mutations on ultraviolet survival and genetic recombination in reD strain of Escherichia coli K-12. Mol. Gen. Genet. 212: 317-324.
32. LOVETT, S. T., and R. D. KOLODNER, 1989 Identification and purification of a single-stranded DNA-specific exonuclease encoded by the recJ gene of Escherichia coli. Proc. Natl. Acad. Sci. USA 86: 2627-2631.
33. LOVETT, S. T., C. LUISI-DE LUCA and R. D. KOLODNER, 1988 The genetic dependence of recombination in recD mutants of Escherichia coli. Genetics 120: 37-45.
34. MAHM, A. A., and R. G. LLOYD, 1989 Identification of the recRlocus of Escherichia coli K-12 and analysis of its role in recombination and DNA repair. Mol. Gen. Genet. 216: 503-510.
35. MARŠIĆ, N., S. ROJE, I. STOJILJKOVIĆ, E. SALAJ-ŠMIC and Ž. TRCOVČEVIĆ, 1993 In vivo studies on the interaction of RecBCD enzyme and λ Gam protein. J. Bacteriol. 175: 4738-4743.
36. MENDONCA, V. M., H. D. KLEPIN and S. W. MATSON, 1995 DNA helicases in recombination and repair: construction of a ΔuvrD ΔhelD ΔrecQ mutant deficient in recombination and repair. J. Bacteriol. 177: 1326-1335.
37. MILLER, J. H., 1992 Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
38. MURPHY, K. C., 1991 λ Gam protein inhibits the helicase and X-stimulated recombination activities of Escherichia coli RecBCD enzyme. J. Bacteriol. 173: 5808-5821.
39. NAKAYAMA, H., K. NAKAYAMA, R. NAKAYAMA, N. IRINO, Y. NAKAYAMA et al., 1984 Isolation and genetic characterization of a thymineless death-resistant mutant of Escherichia coli K 12: identification of a new mutation (recQ1) that blocks the RecF recombination pathway. Mol. Gen. Genet. 195: 474-480.
40. PAŠKVAN, I., E. SALAJ-ŠMIC, I. IVANČIĆ-BAĆE, K. ZAHRADKA, Ž. TRGOVČEVIĆ et al., 2001 The genetic dependence of RecBCD-Gam mediated double strand end repair in Escherichia coli. FEMS Microbiol. Lett. 205: 299-303.
41. 2+. J. Biol. Chem. 254: 8646-8652.
42. RYDER, L., M. C. WHITBY and R. G. LLOYD, 1994 Mutation of recF, recJ, recO, recQ, or recR improves Hfr recombination in resolvase-deficient ruv recG strains of Escherichia coli. J. Bacteriol. 176: 1570-1577.
43. SMITH, G. R., 1989 Homologous recombination in E. coli: multiple pathways for multiple reasons. Cell 58: 807-809.
44. SMITH, G. R., 2001 Homologous recombination near and far from DNA breaks: alternative roles and contrasting views. Annu. Rev. Genet. 35: 243-274.
45. TAYLOR, A. F., and G. R. SMITH, 1992 RecBCD enzyme is altered upon cutting DNA at a chi recombinational hotspot. Proc. Natl. Acad. Sci. USA 89: 5226-5230.
46. TAYLOR, A. F., and G. R. SMITH, 1999 Regulation of homologous recombination: chi inactivates RecBCD enzyme by disassembly of the three subunits. Genes Dev. 13: 890-900.
47. TELANDER-MUSKAVITCH, K. M., and S. LINN, 1982 A unified mechanism for the nuclease and unwinding activities of the RecBC enzyme of Escherichia coli. J. Biol. Chem. 257: 2641-2648.
48. TSENG, Y. C., J. L. HUNG and T. C. V. WANG, 1994 Involvement of the RecF pathway recombination genes in postreplication repair in UV-irradiated Escherichia coli cells. Mutat. Res. 315: 1-9.
49. UMEZU, K., and R. D. KOLODNER, 1994 Protein interactions in genetic recombination in Escherichia coli. Interactions involving RecO and recR overcome the inhibition of RecA by singlestranded DNA binding protein. J. Biol. Chem. 269: 30005-30013.
50. UMEZU, K., K. NAKAYAMA and H. NAKAYAMA, 1990 Escherichia coli RecQ protein is a DNA helicase. Proc. Natl. Acad. Sci. USA 87: 5363-5367.
51. UMEZU, K. N. W. CHI and R. D. KOLODNER, 1993 Biochemical interaction of the Escherichia coli RecF, RecO and RecR proteins with RecA protein and single-stranded DNA binding protein. Proc. Natl. Acad. Sci. USA 90: 3875-3879.
52. WANG, J., R. CHEN and D. A. JULIN, 2000 A single nuclease active site of the Escherichia coli RecBCD enzyme catalyzes single-stranded DNA degradation in both directions. J. Biol. Chem. 275: 507-513.
53. WEBB, B. L., M. M. COX and R. B. INMAN, 1997 Recombinational DNA repair: the RecF and RecR proteins limit the extension of RecA filaments beyond single-strand DNA gaps. Cell 91: 347-356.
54. YU, M., J. SOUAYA and D. A. JULIN, 1998 Identification of the nuclease active site in the multifunctional RecBCD enzyme by creation of a chimeric enzyme. J. Mol. Biol. 283: 797-808.