Influence of prednisolone on the secretion of mucin from the HT29-MTX cell line

Clinical Otolaryngology and Allied Sciences

Volumn 28, Issue 1, 2003, Pages 39-42

  Fergie, Neil ^a   Guo, L ^a   Sithole, J ^b   Pearson, J P ^c   Birchall, J P ^a  

^a QUEEN S MEDICAL CENTRE   (United Kingdom)

^b MRC INSTITUTE OF HEARING RESEARCH   (United Kingdom)

^c NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

Glue; Otitis media with effusion; Steroids

Indexed keywords

GLUCOCORTICOID; MUCIN; PREDNISOLONE; STEROID;

ARTICLE; CELL CULTURE; CELL LINE; CELL STRAIN HT29; CONCENTRATION RESPONSE; CONTROLLED STUDY; HUMAN; HUMAN CELL; INCUBATION TIME; OTITIS MEDIA; PRIORITY JOURNAL; PROTEIN SECRETION; REDUCTION;

EID: 0037291176     PISSN: 03077772     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2273.2003.00656.x     Document Type: Article

References (22)

1. ROSENFELD R.M., MANDEL E.M. & BLUESTONE C.D. (1991) Systemic steroids for otitis media with effusion in children. Arch. Otolaryngol. Head Neck Surg. 117, 984-989
2. LESUFFLEUR T., BARBAT A., DUSSAULX E. et al. (1990) Growth adaptation to methotrexate of HT-29 human colon carcinoma cells is associated with their ability to differentiate into columnar absorptive and mucus-secreting cells. Cancer Res. 50, 6334-6343
3. SMIRNOVA M.G., KISELEV S.L., BIRCHALL J.P. et al. (2001) Up-regulation of mucin secretion in HT29-MTX ells by the pro-inflammatory cytokines tumour necrosis factor and interleukin-6. European Cytokine Network, 12 (1), 119-25
4. KITAMURA H., CHO M., LEE B.H. et al. (1996) Alteration in mucin gene expression and biological properties of HT29 colon cancer cell subpopulations. European J. Cancer 32A (10), 1788-1796
5. FORSTNER G. (1995) Signal transduction, packaging and secretion of mucins. Ann. Rev. Physiol. 57, 585-605
6. HUTTON D.A., FOGG F.J., KUBBA H. et al. (1998) Heterogeneity in the protein cores of mucins isolated from human middle ear effusions: evidence for expression of different mucin gene products. Glycoconjugate J. 15, 283-291
7. GUM J.R. (1995) Mucins: their structure and biology. Biochem. Soc. Transactions. 23, 795-799
8. CARRIE S., HUTTON D.A., BIRCHALL J.P. et al. (1992) Otitis media with effusion: components which contribute to viscous properties. Acta Otolaryngol. 112, 505-511
9. VAN KLINKEN B.J., DEKKER J, BULLER H.A. et al. (1995) Mucin gene structure and expression: protection vs. adhesion. Am. J. Physiol. 269 (5, 1), G613-G627.
10. GOLLUB E.G., WAKSMAN H., GOSWAMI S. et al. (1995) Mucin genes are upregulated by estrogens and dexamethasone. Biochem. Biophys. Res. Comms. 217 (3), 1006-1014
11. FINNIE I.A., CAMPBELL B.J., TAYLOR B.A. et al. (1996) Stimulation of colonic mucin synthesis by corticosteroids and nicotine. Clin. Sci. 91 (3), 359-364
12. KAI H., YOSHITAKE K., HISATSUNE A. et al. (1996) Dexamethasone suppresses mucus production and MUC-2 and MUC5-AC gene expression by NCI-H292 cells. Am. J. Physiol. 271 (3, 1), L484-L488
13. MAROM Z., SHELHAMER J., ALLING D. et al. (1984) The effects of corticosteroids on mucous glycoprotein secretion from human airways in vitro. Am. Rev. Respiratory Dis. 129 (1), 62-65
14. TOBLER A., MEIER R., SEITZ M. et al. (1992) Glucocorticoids downregulate gene expression of GM-CSF, NAP-1/IL-8 and IL-6 but not of M-CSF in human fibroblasts. Blood 79 (1), 45-51
15. CHAUDHARY L.R. & AVIOLI L.V. (1994) Dexamethasone regulates IL-1B and TNF-α-induced IL-8 production in human bone marrow stromal and osteoblast-like cells. Calcif. Tissue Int. 55, 16-20
16. JARRY A., VALLETTE G., BRANKA J.-E. et al. (1996) Direct secretory effect of interleukin-1 via type 1 receptors in human colonic mucous epithelial cells (HT-29-Cl.16E). Gut 38, 240-242
17. KASPER W.J. & HOWE P.M. (1990) Fatal varicella after a single course of corticosteroids. Paediatric Infect. Dis. J. 9, 729-732
18. BUTLER C.C. & VAN DER VOORT J.H. (2001) Steroids for otitis media with effusion. A systematic review. Arch. Pediatr. Adolesc. Med. 155, 641-647
19. HEMLIN C., CARENFELT C. & PAPATZIAMOS G. (1997) Single dose of betamethasone in combined medical treatment of secretory otitis media. Ann. Otol. Rhinol. Laryngol. 106, 359-363
20. SCHWARTZ R.H., PUGLESE J. & SCHWARTZ D.M. (1980) Use of a short course of prednisolone for treating middle ear effusion. A double blind crossover study. Ann. Otol. Rhinol. Laryngol. 89, 296-300
21. ROSENFELD R.M. (1995) Non-surgical management of surgical otitis media with effusion. J. Laryngol. Otol. 109, 811-816
22. ELLIOT P.R., POWELL-TUCK J. & GILLESPIE P.E. (1980) Prednisolone absorption in acute colitis. Gut 21, 49-51