RNA extraction and RT-PCR procedures adapted for the detection of enterovirus sequences from frozen and paraffin-embedded formalin-fixed spinal cord samples

Journal of Virological Methods

Volumn 107, Issue 2, 2003, Pages 115-120

  Beaulieux, Frédérik ^a   Berger, Martina M ^a,b   Tcheng, Rémy ^a   Giraud, Pierric ^a   Lina, Bruno ^a  

^a Domaine Rockefeller   (France)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Enterovirus; RNA extraction; RT PCR; Spinal cord samples

Indexed keywords

FORMALDEHYDE; PARAFFIN; PHOSPHATE; PROTEINASE K; VIRUS RNA;

AMYOTROPHIC LATERAL SCLEROSIS; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; ENTEROVIRUS; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SAMPLING; SPINAL CORD; VIRUS DETECTION;

AMYOTROPHIC LATERAL SCLEROSIS; CRYOPRESERVATION; ENTEROVIRUS; ENTEROVIRUS INFECTIONS; FORMALDEHYDE; HUMANS; PARAFFIN EMBEDDING; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SPINAL CORD; TISSUE FIXATION;

ENTEROVIRUS;

EID: 0037290947     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(02)00209-4     Document Type: Article

References (17)

1. Anonymous, 1989. In vitro amplification of DNA by the polymerase chain reaction. In: Sambrock, J., Fritsch, E.F., Maniatis, T. (Eds.), Molecular Cloning, A Laboratory Manual, Second Ed. Cold Spring Harbor Laboratory Press, New York, p. 14.14.
2. Ben Ezra J., Jonhson D.J., Rossi J., Cook N., Wu A. Effect of fixation on the amplification of nucleic acids from paraffin embedded material by polymerase chain reaction. J. Histochem. Cytochem. 39:1991;351-354.
3. Berger M.M., Kopp N., Vital C., Redl B., Aymard M., Lina B. Detection and cellular localization of enterovirus RNA sequences in spinal cord of patients with ALS. Neurology. 54:2000;20-25.
4. Brahic M., Smith R.A., Gibbs C.J. Jr, Garruto R.M., Tourtellotte W.W., Cash E. Detection of picornavirus sequences in nervous tissue of amyotrophic lateral sclerosis and control patients. Ann. Neurol. 18:1985;337-343.
5. Chomczynsky P., Sacchi N. Single-step method of RNA isolation by guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:1987;156-169.
6. Cimino G.D., Metchette K.C., Tessman J.W., Hearst J.E., Isaaca J.T. Post-PCR sterilization: a method to control carry-over contamination for the polymerase chain reaction. Nucleic Acids Res. 19:1990;99-107.
7. Foss R.D., Thakurta N.G., Conran R.M., Gutman P. Effects of fixative and fixation time on the extraction and polymerase chain reaction amplification of RNA from paraffin embedded tissue. N. Diag. Mol. Pathol. 3:1994;148-155.
8. Gratsch T.E., Gray V.F. Construction of a recombinant cDNA of echovirus 6 that established a persistent in vitro infection. Virology. 201:1994;341-348.
9. Giraud P., Beaulieux F., Ono S., Shimizu N., Chazot G., Lina B. Detection of enteroviral sequences from frozen spinal cord samples of Japanese ALS patients. Neurology. 56:2001;1777-1778.
10. Karpati G., Dalakas M.C. Viral hide and seek in sporadic ALS: a new challenge. Neurology. 54:2000;6-7.
11. Leparc I., Aymard M., Fuchs F. Acute chronic and persistent enterovirus and poliovirus infections: detection of viral genome by semi-nested PCR amplification in culture negative samples. Mol. Cell. Probes. 8:1994;487-495.
12. Lina B., Pozzetto B., Andreoletti L., Beguier E., Bourlet T., Dussaix E., Grangeot-Keros L., Gratacap-Cavalier B., Henquell C., Legrand-Quillien M.C., Novillo A., Palmer P., Petitjean J., Sandres K., Dubreuil P., Fleury H., Freymuth F., Leparc-Goffart I., Hober D., Izopet J., Kopecka H., Lazizi Y., Lafeuille H., Lebon P., Roseto A., Marchadier E., Masquelier B., Picard B., Puel J., Seigneurin J.M., Wattre P., Ayamrd M. Multicenter evaluating of a commercially available PCR assay for diagnosing enterovirus infection in a panel of cerebrospinal fluid specimens. J. Clin. Microbiol. 34:1996;3002-3006.
13. Muir P., Nicholson F., Spencer G.T. Enterovirus infection of the central nervous system of humans: lack of association with chronic neurological disease. J. Gen. Virol. 77:1996;1469-1476.
14. Muir P., Ras A., Klapper P.E., Cleator G.M., Korn K., Aepinus C., Formsgaard A., Palmer P., Samuelsson A., Tenorio A., Weissbrich B., v Loon A.M. Multicenter quality assessment of PCR methods for detection of enterovirus. J. Clin. Microbiol. 37:1999;1409-1414.
15. Righthand V.F., Blackburn R.V. Steady-state infection by echovirus 6 associated with non-lytic viral RNA and unprocessed capsid polypeptide. J. Virol. 63:1989;5268-5275.
16. Swanson N.R., Fox S.A., Mastaglia F.L. Search for persistent infection with poliovirus or other enteroviruses in amyotrophic lateral sclerosis-motor neuron disease. Neuromuscular Disord. 5:1995;457-465.
17. Woodall C.J., Riding M.H., Graham D.I., Clements G.B. Sequences specific for enterovirus detected in spinal cord from patients with motor neuron disease. Br. Med. J. 308:1994;1541-1543.