Introduction of the 5-HT3B subunit alters the functional properties of 5-HT3 receptors native to neuroblastoma cells

Neuropharmacology

Volumn 44, Issue 2, 2003, Pages 214-223

  Stewart, A ^a   Davies, P A ^a   Kirkness, E F ^b   Safa, P ^a,c   Hales, T G ^a  

^a GEORGE WASHINGTON UNIVERSITY SCHOOL OF MEDICINE AND HEALTH SCIENCES   (United States)

^b J CRAIG VENTER INSTITUTE   (United States)

^c STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

5 HT3 receptors; 5 Hydroxytryptamine; Calcium imaging; Neuroblastoma; Patch clamp; Serotonin

Indexed keywords

1 (3 CHLOROPHENYL)BIGUANIDE; 2 METHYLSEROTONIN; CAFFEINE; CALCIUM ION; MESSENGER RNA; ONDANSETRON; RECEPTOR SUBUNIT; SEROTONIN; SEROTONIN 3 AGONIST; SEROTONIN 3 RECEPTOR; THAPSIGARGIN; 1-(3-CHLOROPHENYL)BIGUANIDE; 2 METHYL 5 HT; 2-METHYL-5-HT; BIGUANIDE DERIVATIVE; CALCIUM; DRUG DERIVATIVE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PHOTOPROTEIN; PROTEIN SUBUNIT; RECOMBINANT PROTEIN; SEROTONIN AGONIST; SEROTONIN ANTAGONIST; SEROTONIN RECEPTOR;

ANIMAL CELL; ARTICLE; CALCIUM CELL LEVEL; CELL LINE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG EFFICACY; GENETIC CODE; GENETIC TRANSFECTION; KINETICS; MOUSE; NEUROBLASTOMA CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; RECORDING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SLOW INWARD CURRENT; ANIMAL; CELL DIFFERENTIATION; CHEMISTRY; CLASSIFICATION; COMPARATIVE STUDY; DOSE RESPONSE; DRUG EFFECT; DRUG INTERACTION; FLUORESCENCE; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR GENETICS; NEUROBLASTOMA; PATCH CLAMP; PHYSIOLOGY; RAT;

ANIMALS; BIGUANIDES; CALCIUM; CELL DIFFERENTIATION; CELL LINE; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG INTERACTIONS; FLUORESCENCE; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MICE; MOLECULAR SEQUENCE DATA; NEUROBLASTOMA; ONDANSETRON; PATCH-CLAMP TECHNIQUES; PROTEIN SUBUNITS; RATS; RECEPTORS, SEROTONIN; RECEPTORS, SEROTONIN, 5-HT3; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SEROTONIN; SEROTONIN AGONISTS; SEROTONIN ANTAGONISTS; TRANSFECTION;

EID: 0037289607     PISSN: 00283908     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0028-3908(02)00376-3     Document Type: Article

References (32)

1. 3 recognition sites within the human brainstem. Neuroscience Letters. 111:1990;80-86.
2. 3 receptor. J. Neurochem. 64:1995;1401-1405.
3. 3 receptor in human forebrain. Neuropharmacology. 32:1993;1325-1331.
4. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:1987;156-159.
5. 3B subunit is a major determinant of serotonin-receptor function. Nature (Lond.). 397:1999;359-363.
6. A receptor assembly and pharmacology in WSS-1 cells and transiently transfected HEK293 cells. Neuropharmacol. 39:2000;611-620.
7. 2+. J. Biol. Chem. (in press).
8. 3B receptor subunit. J. Biol. Chem. 274:1999;30799-30810.
9. 3 receptors really homomeric complexes? Trends Pharmacol. Sci. 19:1998;212-215.
10. Furuya K., Furuya S., Yamagishi S. Developmental time course of Na and Ca spikes in neuroblastoma X glioma hybrid cells. Brain Res. 313:1983;229-234.
11. 2+ channels in PC12 cells by nerve growth factor and cAMP. Neurosci. Res. 16:1993;143-147.
12. Hales T.G., Kim H., Longoni B., Olsen R.W., Tobin A.J. Immortalized hypothalamic GT1-7 neurons express functional gamma-aminobutyric acid type A receptors. Mol. Pharmacol. 42:1992;197-202.
13. A mRNAs have functional receptors. J. Neurosci. 14:1994;5429-5436.
14. 3 receptors in rodents. J. Neurochem. 75:2000;240-247.
15. 2+ permeability of cloned and native 5-hydroxytryptamine type 3 receptors. Mol. Phamacol. 46:1994;1120-1128.
16. 3 recognition sites in membranes of N1E-115 neuroblastoma cells by radioligand binding. Mol. Pharmacol. 33:1988;303-309.
17. Hussy N., Lukas W., Jones K.A. Functional properties of a cloned 5-hydroxytryptamine ionotropic receptor subunit: comparison with native mouse receptors. J. Physiol. 481:1994;311-323.
18. 3 receptors in clonal cell lines studied by patch-clamp techniques. Br J. Pharmacol. 97:1989;27-40.
19. 3 receptor, a serotonin-gated ion channel. Science. 254:1991;432-454.
20. 3-receptor subunits A and B are co-expressed in neurons of the dorsal root ganglion. J. Comp. Neurol. 438:2001;163-172.
21. Prasad K.N., Kumar S. Role of cyclic AMP in differentiation of human neuroblastoma cells in culture. Cancer. 36:1975;1338-1343.
22. 3 receptor-induced currents in N1E-115 neuroblastoma cells. European J. Pharmacol. 151:1988;491-495.
23. A receptor-mediated responses in the human IMR-32 neuroblastoma cell line. J. Neurosci. Res. 60:2000;504-510.
24. 3 receptor. J. Neurophysiol. 65:1991;630-638.
25. Simonneau M., Distasi C., Tauc L., Poujeol C. Development of ionic channels during mouse neuronal differentiation. J. Physiol. (Paris). 80:1985;312-320.
26. 3 receptors in N1E-115 neuroblastoma cells. Br J. Pharmacol. 104:1991;536-540.
27. A receptor subunit mRNAs in 13 cell lines. J Neurosci. 14:1994;5417-5428.
28. Unwin N. The structure of ion channels in membranes of excitable cells. Neuron. 3:1989;665-676.
29. 2+-permeable ion channels. Proc. Natl. Acad. Sci. USA. 95:1998;11456-11461.
30. 3 receptor. Brain Res. 533:1990;46-52.
31. Yang J. Ion permeation through 5-hydroxytryptamine gated channels in neuroblastoma N18 cells. J. Gen. Physiol. 448:1990;1177-1198.
32. 3 receptor channels in dissociated rat superior cervical ganglion neurons. J. Physiol. 448:1992;237-256.