Acute toxicity assessment of azadirachtin-based pesticides using murine hybridoma and oyster cells

Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes

Volumn 38, Issue 2, 2003, Pages 169-180

  Goktepe, I ^a,b   Plhak, L C ^a,c  

^a LOUISIANA STATE UNIVERSITY AGRICULTURAL CENTER   (United States)

^b NORTH CAROLINA A AND T STATE UNIVERSITY   (United States)

^c UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Azadirachtin; Daphnia pulex; Hybridoma; Neem pesticides; Oyster cells

Indexed keywords

CELLS; EXTRACTION; TOXICITY;

OYSTER CELLS;

PESTICIDES;

AZADIRACHTIN;

PESTICIDE; TOXICITY TEST;

ACUTE TOXICITY; ANIMAL CELL; ARTICLE; BIOLOGICAL MONITORING; CRUSTACEA; ECOSYSTEM; ENVIRONMENTAL EXPOSURE; GREENHOUSE EFFECT; GROWTH INHIBITION; HYBRIDOMA; MOUSE; NONHUMAN; OYSTER; PESTICIDE SPRAYING; REPRODUCIBILITY; TOXICITY TESTING;

ACUTE TOXICITY TESTS; ANIMALS; BIOLOGICAL ASSAY; DAPHNIA; DOSE-RESPONSE RELATIONSHIP, DRUG; HYBRIDOMAS; INSECTICIDES; LETHAL DOSE 50; LIMONINS; MICE; OSTREIDAE;

ANIMALIA; AZADIRACHTA INDICA; CRUSTACEA; DAPHNIA; DAPHNIA PULEX; INVERTEBRATA; MURINAE; OSTREIDAE; PULEX;

EID: 0037285731     PISSN: 03601234     EISSN: None     Source Type: Journal    
DOI: 10.1081/PFC-120018447     Document Type: Article

References (30)

1. Schmutterer, H.; Ascher, K.R.S.; Rembold, H. In Natural Pesticides from the Neem Tree, Proceedings of the 1st International Neem Conference, Rottach-Egem, Germany, 1980; Schmutterer, H., Ascher, K.R.S., Rembold, H., Eds.; GTZ: Eschborn, Germany, 1981; 63-74.
2. Schmutterer, H.; Ascher, K.R.S.; Rembold, H. In Natural Pesticides from the Neem Tree, Proceedings of the 1st International Neem Conference, Rottach-Egem, Germany, 1980; Schmutterer, H., Ascher, K.R.S., Rembold, H., Eds.; GTZ: Eschborn, Germany, 1981; 63-74.
3. Jacobson, M. Focus on Phytochemical Pesticides: the Neem Tree; Jacobson, M., Ed.; CRC Press: Boca Raton, FL, 1989; Vol. I, 69-86.
4. Ara, I.; Siddiqui, B.S.; Faizi, S.; Siddiqui, S. Two new terpenoids from root bark of Azadirachta indica. J. Nat. Prod. 1989, 52 (8), 1209-1213.
5. Van Der Nat, J.M.; Van Der Sluis, W.G.; De Silva, K.T.D.; Labadie, R.P. Ethnopharmacognostical survey of Azadirachta indica. J. Ethnopharmacol. 1991, 35, 1-24.
6. Williams, J.R.; Peng, C.Y.S.; Chuand, R.Y.; Doi, R.H.; Mussen, E.C. The inhibitory effect of Azadirachtin on Bacillus subtilus, Escherichia coli, and Paenibacillus larvae, the causative agent of American foulbrood in the honeybee, Apis mellifera L. J. Inverteb. Pathol. 1998, 72 (3), 252-257.
7. Isman, M.B. Neem insecticides. Pestic. Outlook 1997, 8 (5), 32-38.
8. Kraus, W.; Bokel, M.; Bruhn, A.; Cramer, R.; Klaiber, I.; Klenck, A.; Nagl, G.; Pohnl, H.; Sadlo, H.; Vogler, B. Structure determination by NMR of azadirachtin and related compounds from Azadirachta indica. Tetrahedron 1987, 43 (12), 2817-2830.
9. Luntz, A.J.M.; Backwell, A. Azadirachtin: an update, a review. J. Insect Physiol. 1993, 39 (10), 903-924.
10. Low-Friedrich, I.; Von Bredow, K.; Schoeppe, W. A cell culture assay for the detection of cardiotoxicity. J. Pharmacol. Methods 1991, 25 (2), 133-145.
11. Schmutterer, H. Side effects on beneficial and other ecologically important non-target organisms. In The Neem Tree Azadirachta Indica and Other Meliaceous Plants; Schmuttterer, H., Ed.; VCH: Weinheim, 1995; 495-517.
12. Bertheussen, K.; Yousef, M.I.; Figenschau, Y. A new sensitive cell culture test for the assessment of pesticide toxicity. J. Environ. Sci. Health, Part B, Pestic. Food Contam. Agric. Wastes 1997, 32 (2), 195-211.
13. Goktepe, I. Toxicity of neem-based insecticides on aquatic animals and cell lines. Louisiana State University Dissertation; 1999.
14. Plhak, L.C.; Sporns, P. Development and production of monoclonal antibodies for the measurement of solanidine potato glycoalkaloids. Am. Potato J. 1994, 71, 297-313.
15. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65, 55-63.
16. Buchanan, J.T.; Roppolo, G.S.; Supan, J.E.; Tiersch, T.R. Conditioning of eastern oyster in a closed, recirculating system. J. Shellfish Res. 1998, 17 (4), 1183-1189.
17. Buchanan, J.T.; LaPeyre, J.F.; Cooper, R.K.; Tiersch, T.R. Improved attachment and spreading in primary cell cultures of the eastern oyster, Crassostrea virginica in vitro. Cell Dev. Biol. Anim. 1999, 35 (10), 593-598.
18. Buttke, T.M.; McCubrey, J.A.; Owen, T.C. Use of an aqueous soluble tetrazolium/formazan assay to measure viability and proliferation of lymphokine-dependent cell line. J. Immunol. Methods 1993, 157, 233-240.
19. USEPA. Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms, 4th Ed.; Weber, C.I., Ed.; U.S. Environmental Protection Agency: Cincinnati, OH, 1993; 38-94. EPA/600/4-90/027F.
20. SAS Institute Inc., SAS®. User's Guide: Statistics; 1995 Ed.; SAS Institute, Inc.: Cary, NC, 1995.
21. Cohen, E.; Quistad, G.B.; Jefferies, P.R.; Casida, J.E. Nimbolide is the principal cytotoxic component of neem-seed insecticide preparations. Pestic. Sci. 1996, 48 (2), 135-140.
22. Juneja, S.C.; Pfeifer, T.; Williams, R.S.; Chegini, N. Neem oil inhibits 2-cell embryo development and trophectoderm attachment and proliferation in vitro. J. Assist. Reprod. Genet. 1994, 11 (8), 419-427.
23. Rembold, H.; Annadurai, R.S. Azadirachtin inhibits proliferation of Sf9 cells in monolayer culture. Z. Naturforsch. 1993, 48, 495-499.
24. Akudugu, J.; Gade, G.; Bohm, L. Cytotoxicity of azadirachtin A in human glioblastoma cell lines. Life Sci. 2001, 68, 1153-1160.
25. Goktepe, I.; Plhak, L.C. Comparative toxicity of two azadirachtin-based neem pesticides to Daphnia pulex. Environ. Toxicol. Chem. 2002, 21 (1), 31-36.
26. Larson, R.O. The commercialization of neem. In Focus on Phytochemical Pesticides: the Neem Tree; Jacobson, M., Ed.; CRC Press: Boca Raton, 1989; Vol. I, 155-168.
27. Stark, J.D. Population-level effects of the neem insecticide, Neemix, on Daphnia pulex. J. Environ. Sci. Health, Part B, Pestic. Food Contam. Agric. Wastes 2001, 36 (4), 457-465.
28. Leight, A.K.; Dolah, R.F.V. Acute toxicity of the insecticides endosulfan, chlorpyrifos, and malathion to the epibenthic estuarine amphipod Gammarus palustris. Environ. Toxicol. Chem. 1999, 18 (5), 958-964.
29. Tomlin, C. The Pesticide Manual, Incorparating the Agrochemicals Handbook; 10th Ed.; The Royal Society of Chemistry and British Crop Protection Council: Cambridge, U.K., 1994; 115-124.
30. Stratton, G.; Corke, G. Interaction of permethrin with Daphnia magna in the presence and absence of particulate material. Environ. Pollut. 1981, 24, 135-144.