Standardisation and comparison of serial dilution and single dilution enzyme linked immunosorbent assay (ELISA) using different antigenic preparations of the Babesia (Theileria) equi parasite

Veterinary Research

Volumn 34, Issue 1, 2003, Pages 71-83

  Kumar, Sanjay ^a,b   Kumar, Yogesh ^a   Malhotra, Dharam V ^a   Dhar, Shruti ^a   Nichani, Anil K ^a,c  

^a CCS HARYANA AGRICULTURAL UNIVERSITY   (India)

^b OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE   (Japan)

^c UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

Babesia equi; ELISA; Protozoan; Sero diagnosis; Theileria equi

Indexed keywords

PARASITE ANTIGEN; PROTOZOON ANTIBODY;

ANIMAL DISEASE; ANTIBODY DETECTION; ANTIBODY TITER; ARTICLE; BABESIA CABALLI; BABESIA EQUI; BABESIOSIS; CALCULATION; CELL MEMBRANE; CONTROLLED STUDY; CORRELATION COEFFICIENT; CROSS REACTION; DIAGNOSTIC VALUE; DILUTION; DONKEY; ECONOMIC ASPECT; ENZYME LINKED IMMUNOSORBENT ASSAY; HORSE; INTERMETHOD COMPARISON; MEROZOITE; NONHUMAN; OPTICAL DENSITY; PREDICTION; PROTOZOAL INFECTION; REGRESSION ANALYSIS; SENSITIVITY AND SPECIFICITY; SERODIAGNOSIS; STANDARDIZATION; SUPERNATANT; VALIDATION PROCESS;

ANIMALIA; BABESIA CABALLI; BABESIA EQUI; EQUIDAE; EQUUS ASINUS; EQUUS CABALLUS; PROTOZOA; THEILERIA;

EID: 0037283720     PISSN: 09284249     EISSN: None     Source Type: Journal    
DOI: 10.1051/vetres:2002055     Document Type: Article

References (35)

1. Beniwal R.P., Nichani A.K., Rakha N.K., Sharma R.D., Sarup S., An immunisation trial with in-vitro produced Babesia bigemina exoantigens, Trop. Anim. Health Prod. 29 (1997) 124S-126S.
2. Bose R., Peymann B., Diagnosis of Babesia caballi infections in horse by enzyme-linked immunosorbent assay (ELISA) and western blot, Int. J. Parasitol. 24 (1994) 347-356.
3. Briggs D.J., Skeeles J.K., An enzyme-linked immunosorbant assay for detecting antibodies to Pasteurella multocida in chickens, Avian Dis. 28 (1984) 208-215.
4. Brooks L.M., The equine piroplasmosis control programme at the 1996 Summer Olympic Games. Equine Infectious Diseases VIII. Proceedings of the Eighth International Conference, Dubai, 1998, pp. 371-375.
5. Brown W.C., Kathleen S.L., Gerald G.W., Tetzlaff C.L., Cell mediated immune response to Babesia equi merozoite antigen in cattle following infection with tick-derived or cultured parasite, Infect. Immun. 59 (1991) 2418-2426.
6. Bruning A., Phipps P., Posnett E., Canning E.U., Monoclonal antibodies against Babesia caballi and Babesia equi and their application in serodiagnosis, Vet. Parasitol. 68 (1997) 11-26.
7. Dennig H.K., Brocklesby D.W., Splenectomy of horses and donkeys, Vet. Rec. 77 (1965) 40-45.
8. Donnelly J., Phipps L.P., Evidence of maternal antibodies to Babesia equi and Babesia caballi in foals of seropositive mares, Equine Vet. J. 14 (1982) 126-128.
9. Frerich W.M., Holbrook A.A., Johnson A.J., Equine piroplasmosis: complement-fixation titres of horses infected with Babesia caballi, Am. J. Vet. Res. 30 (1969) 697-702.
10. Frerich W.M., Holbrook A.A., Johnson A.J., Equine piroplasmosis: production of antigens for the complement-fixation test, Am. J. Vet. Res. 30 (1969) 1337-1341.
11. Friedhoff K.T., Soule C., An account on equine babesiosis, Rev. Sci. Tech. Off. Int. Epizoot. 15 (1996) 1191-1201.
12. Gautam O.P., Dwivedi S.K., Equine babesiosis: A severe out-break in a stud farm at Hissar, Indian Vet. J. 53 (1976) 546-551.
13. Heuchert C.M.S., de Giulli Jr. V., de Athaide D.F., Bose R., Friedhoff K.T., Seroepidemiology studies on Babesia equi and Babesia caballi infections in Brazil, Vet. Parasitol. 85 (1999) 1-11.
14. Hirato K., Ninomiya M., Uwano Y., Kuth T., Studies on the complement-fixation reaction for equine piroplasmosis, Jpn. J. Vet. Sci. 7 (1945) 197-205.
15. Holman P.J., Chieves L., Frerichs W.M., Olson D., Wagner G.G., Culture confirmation of the carrier status of Babesia equi infected horses, J. Clin. Microbiol. 31 (1993) 698-701.
16. Kerber C.E., Ferreira F., Pereira M.C., Efficacy of imidocarb against natural Babesia equi infections in horses. Equine Infectious Diseases VIII. Proceedings of the Eighth International Conference, Dubai, 1998, pp. 552-554.
17. Khatri N., Nichani A.K., Sharma R.D., Khatri M., Malhotra. D.V., Effect of vaccination on young calves born during winter season in the field with the Theileria annulata (Hisar) cell culture vaccine, Vet. Res. Commun. 25 (2001) 179-188.
18. Knowles D.P., Perryman L.E., Goff W.L., Miller C.D., Harrington R.D., Gorham J.R., A monoclonal antibody defines a geographically conserved surface protein epitope of Babesia equi merozoites, Infect. Immun. 59 (1991) 2412-2417.
19. Kumar S., Malhotra D.V., Dhar S., Serodiagnosis of Babesia equi infection - a comparison of Dot-ELISA, complement fixation test and capillary tube agglutination test, Vet. Parasitol. 69 (1997) 171-176.
20. Levine N.D., in: Veterinary Protozoology, Iowa State University Press, Ames, Iowa, 1985.
21. Lowry O.H., Rosebrough N.J., Farr A., Randall R.J., Protein estimation with the Folin Phenol reagent, J. Biol. Chem. 195 (1951) 265-275.
22. Madden P.A., Holbrook A.A., Equine piroplasmosis: indirect fluorescent antibody test for Babesia caballi, Am. J. Vet. Res. 29 (1968) 117-119.
23. Manual of recommended diagnostic techniques and requirements for biological products for lists A and B diseases, Office International Des Epizooties, Vol. I, 1989, 1/6-6/6.
24. Manuja A., Nichani A.K., Kumar R., Sharma R.D., Kumar B., Single dilution ELISAs using piroplasm, cellular schizont and soluble schizont antigens for the detection of antibodies against Theileria annulata, Vet. Res. 32 (2001) 165-173.
25. Mc Guire T.C., Van Hoosier G.L., Henson J.B., The complement-fixation reaction in equine infectious anaemia demonstration of inhibition by IgG (T), J. Immunol. 107 (1971) 1738-1744.
26. Sharma R.D., Gautam O.P., Clinical cases of equine babesiosis, Haryana Vet. XVI (1977) 19-26.
27. Sharma R.D., Tanwar R.K., Gahlot A.K., Yadav J.S., Rajvanshi D.S., Note on clinical studies in Babesia equi infection in ponies, Indian J. Anim. Sci. 52 (1982) 1001-1003.
28. Singh S., Khatri N., Manuja A., Sharma R.D., Malhotra D.V., Nichani A.K., Impact of field vaccination with a Theileria annulata schizont cell culture vaccine on the epidemiology of tropical theileriosis, Vet. Parasitol. 101 (2001) 91-100.
29. Snedecor G.W., Cochra W.G., Statistical Methods, 8th Edition Iowa University Press, Ames, Iowa, 1989.
30. Tenter A.M., Friedhoff K.T., Serodiagnosis of experimental and natural Babesia equi and Babesia caballi infections, Vet. Parasitol. 20 (1986) 49-61.
31. Tyler J.M., Cullor J.S., Titers, tests, and truisms: rational interpretation of diagnostic serological testing, J. Am. Vet. Med. Assoc. 194 (1989) 1550-1558.
32. Weiland G., Species-specific serodiagnosis of equine piroplasmosis infections by means of complement fixation test, immunofluorescence and enzyme-linked immunosorbant assay, Vet. Parasitol. 20 (1986) 43-48.
33. Westbury H.A., Chamnanpood P., Tangchaitrong S., Doughty W.J., Single dilution ELISA for detection of serum antibody to foot-and-mouth disease virus in cattle, Vet. Microbiol. 18 (1988) 273-283.
34. Zwart D., Brocklesby D.W., Babesiosis: Non-specific resistance, immunological factors and pathogenesis, Adv. Parasitol. (1979) 49-113.
35. Zweygarth E, Just M.C., Waal D.T., Continuous in vitro cultivation of erythrocytic stages of Babesia equi, Parasitol. Res. 81 (1995) 355-358.