Clostridium difficile toxin B, an inhibitor of the small GTPases Rho, Rac and Cdc42, influences spiral ganglion neurite outgrowth

Acta Oto-Laryngologica

Volumn 123, Issue 1, 2003, Pages 20-25

  Brors, Dominik ^a,b   Aletsee, Christoph ^b   Dazert, Stefan ^c   Huverstuhl, Jochen ^b   Ryan, Allen F ^a,d   Bodmer, Daniel ^a  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

^b UNIVERSITY OF WÜRZBURG   (Germany)

^c ST JOSEF HOSPITAL   (Germany)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cdc42; Laminin; NT 3; Rac; Rho; Spiral ganglion

Indexed keywords

CLOSTRIDIUM TOXIN; ENZYME INHIBITOR; GUANOSINE TRIPHOSPHATASE; LAMININ 1; NEUROTROPHIN 3; PROTEIN CDC42; RAC PROTEIN; RHO FACTOR;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CLOSTRIDIUM DIFFICILE; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVATION; EXPLANT; IMMUNOCYTOCHEMISTRY; NERVE FIBER GROWTH; NEWBORN; NONHUMAN; PERINATAL PERIOD; PRIORITY JOURNAL; RAT; SPIRAL GANGLION; TISSUE CULTURE;

ANIMALS; ANIMALS, NEWBORN; BACTERIAL PROTEINS; BACTERIAL TOXINS; CDC42 GTP-BINDING PROTEIN; CELL DIFFERENTIATION; ENZYME ACTIVATION; IMAGE PROCESSING, COMPUTER-ASSISTED; LAMININ; MICROSCOPY, FLUORESCENCE; MONOMERIC GTP-BINDING PROTEINS; NEURITES; NEURONAL PLASTICITY; NEURONS; NEUROTROPHIN 3; RAC GTP-BINDING PROTEINS; RATS; RATS, SPRAGUE-DAWLEY; RHO GTP-BINDING PROTEINS; SIGNAL TRANSDUCTION; SPIRAL GANGLION;

EID: 0037274917     PISSN: 00016489     EISSN: None     Source Type: Journal    
DOI: 10.1080/003655402000028055     Document Type: Article

References (25)

1. Cosgrove D, Rodgers KD. Expression of the major basement membrane-associated proteins during postnatal development in the murine cochlea. Hear Res 1997; 105: 159-70.
2. Pirvola U, Ylikoski J, Palgi J, Lehtonen E, Arumae U, Saarma M. Brain-derived neurotrophic factor and neurotrophin 3 mRNAs in the peripheral target fields of developing inner ear ganglia. Proc Natl Acad Sci USA 1992; 89: 9915-9.
3. Dazert S, Kim D, Luo L, et al. Focal delivery of fibroblast growth factor-1 by transfected cells induces spiral ganglion neurite targeting in vitro. J Cell Physiol 1998; 177: 123-9.
4. Farinas I, Jones KR, Backus C, Wang XY, Reichard LF. Severe sensory and sympathetic deficits in mice lacking neurotrophin-3. Nature 1994; 369: 658-61.
5. Aletsee C, Mullen L, Kim D, et al. The disintegrin kistrin inhibits neurite extension from spiral ganglion explants cultured on laminin. Audiol Neurootol 2001; 6: 57-65.
6. Venstrom KA, Reichardt LF. Extracellular Matrix 2: role of extracellular matrix molecules and their receptors in the nervous system. FASEB J 1993; 7: 996-1003.
7. Aletsee C, Beros A, Mullen L, et al. Ras/MEK but not p38 signaling mediates NT-3-induced neurite extension from spiral ganglion neurons. J Assoc Res Otolaryngol 2001; 2: 377-87.
8. Cary LA, Han DC, Guan JL. Integrin mediated signal transduction pathways. Histol Histopathol 1999; 14: 1001-19.
9. Doherty P, Williams G, Williams EJ. CAMs and axonal growth: a critical evaluation of the role of calcium and the MAPK cascade. Mol Cell Neurosci 2000; 16: 283-95.
10. Chuang TH, Hahn KM, Lee JD, Danley DE, Bokoch GM. The small GTPase CDc42 initiates an apoptotic signalling pathway in Jurkat T lymphocytes. Mol Biol Cell 1997; 8: 1687-98.
11. Bazenet CE, Mota MA, Rubin IL. The small GTP-binding protein Cdc42 is required for nerve growth factor withdrawal induced neuronal death. Proc Natl Acad Sci USA 1998; 95: 3984-9.
12. Wahl S, Barth H, Ciossek T, Aktories K, Mueller BK. Ephrin-A5 induces collapse of growth cones by activating Rho and Rho kinase. J Cell Biol 2000; 149: 263-70.
13. Cameron LA, Giardini PA, Soo FS, Theriot JA. Secrets of actin based motility revealed by a bacterial pathogen. Nat Rev Cell Biol 2000; 1: 110-9.
14. Van De Water TR, Ruben RJ. Organ culture of the mammalian inner ear. Acta Otolaryngol (Stockh) 1971; 71: 303-12.
15. Brors D, Aletsee C, Schwager K, et al. Interaction of spiral ganglion neuron processes with alloplastic materials in vitro. Hear Res 2002; 167(1-2); 110-21.
16. Levebvre PP, Weber T, Rigo JM, Staecker H, Moonen G, Van De Water TR. Peripheral and central target derived trophic factor effects on auditory neurons. Hear Res 1992; 58: 185-92.
17. Nobes CD, Hall A. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 1995; 81: 53-62.
18. Olson MF, Ashworth A, Hall A. An essential role for Rho, Rac, and Cdc42 GTPases in cell cycle progression through G1. Science 1995; 269: 1270-2.
19. Fukata M, Kuroda S, Nakagawa M, et al. Cdc42 and Rac1 regulate the interaction of IQGAP1 with beta-catenin. J Biol Chem 1999; 274: 26044-50.
20. Threadgill R, Bobb K, Ghosh A. Regulation of dendritic growth and remodeling by Rho, Rac, and Cdc42. Neuron 1997; 19: 625-34.
21. Pai EF, Kabsch W, Krengel U, Holmes KC, John J, Wittinghofer A. Structure of the guanine-nucleotide-binding domain of the Ha-ras oncogene product p21 in the triphosphate conformation. Nature 1989; 341: 209-14.
22. Weston CA, Anova L, Rialas C, Prives JM, Weeks BS. Laminin-1 activates Cdc42 in the mechanism of laminin-1-mediated neurite outgrowth. Exp Cell Res 2000; 260: 374-8.
23. Kozma R, Sarner S, Ahmed S, Lim L. Rho family GTPases and neuronal growth cone remodelling: relationship between increased complexity induced by Cdc42Hs, Rac1, and acetylcholine and collapse induced by RhoA and lysophosphatidic acid. Mol Cell Biol 1997; 17: 1201-11.
24. Bodmer D, Brors D, Pak K, Gloddek B, Ryan AF. Rescue of auditory hair cells from aminoglycoside toxicity by Clostridium difficile Toxin B, an inhibitor of the small GTPases Rho/Rac/Cdc42. Hear Res 2002; In press.
25. Ernfors P, Duan ML, ElShamy WM, Canlon B. Protection of auditory neurons from aminoglycoside toxicity by neurotrophin-3. Nat Med 1996; 2: 463-7.