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Volumn 19, Issue 1, 2003, Pages 9-13

Sustained high-yield production of recombinant proteins in transiently transfected COS-7 cells grown on trimethylamine-coated (Hillex) microcarrier beads

Author keywords

[No Author keywords available]

Indexed keywords

MICROCARRIERS; TRANSFECTED CELLS;

EID: 0037274093     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp020092r     Document Type: Article
Times cited : (7)

References (14)
  • 1
    • 0025981417 scopus 로고
    • Gycosylation of recombinant protein therapeutics: Control and functional implications
    • Cumming, DA. Gycosylation of recombinant protein therapeutics: Control and functional implications. Glycobiology 1991, 1, 115-130.
    • (1991) Glycobiology , vol.1 , pp. 115-130
    • Cumming, D.A.1
  • 2
    • 0346856283 scopus 로고    scopus 로고
    • Transfection of DNA into eukaryotic cells
    • Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., Struhl, K., Eds.; Wiley-Interscience: New York, Unit 9.2
    • Gulick, T. Transfection Of DNA Into Eukaryotic Cells In Current Protocols in Molecular Biology; Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., Struhl, K., Eds.; Wiley-Interscience: New York, Unit 9.2, p 2001.
    • Current Protocols in Molecular Biology , pp. 2001
    • Gulick, T.1
  • 4
    • 0027139640 scopus 로고
    • Evaluation of anchorage-dependent cell propagation systems for production of human acetylcholinesterase by recombinant 293 cells
    • Lazar, A.; Reuveny, S.; Kronman, C.; Velan, B.; Shafferman, A. Evaluation of anchorage-dependent cell propagation systems for production of human acetylcholinesterase by recombinant 293 cells. Cytotechnology 1993, 13, 115-123.
    • (1993) Cytotechnology , vol.13 , pp. 115-123
    • Lazar, A.1    Reuveny, S.2    Kronman, C.3    Velan, B.4    Shafferman, A.5
  • 6
    • 0028362305 scopus 로고
    • High-efficiency gene transfer mediated by adenovirus-polylysine DNA complex
    • Curiel, D. T. High-efficiency gene transfer mediated by adenovirus-polylysine DNA complex. Ann. N. Y. Acad. Sci. 1994, 716, 36-56.
    • (1994) Ann. N. Y. Acad. Sci. , vol.716 , pp. 36-56
    • Curiel, D.T.1
  • 7
    • 0023711798 scopus 로고
    • Calcium phosphate-mediated gene transfer: A highly-efficient transfection system for stably transforming cells with plasmid DNA
    • Chen, C. A.; Okayama, H. Calcium phosphate-mediated gene transfer: a highly-efficient transfection system for stably transforming cells with plasmid DNA. BioTechniques 1988, 6, 632-638.
    • (1988) BioTechniques , vol.6 , pp. 632-638
    • Chen, C.A.1    Okayama, H.2
  • 8
    • 0030768650 scopus 로고    scopus 로고
    • Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery
    • Baker, A.; Saltik, M.; Lehrmann, H.; Killisch, I.; Mautner, V.; Lamm, G.; Christofori, G.; Cotton, M. Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery. Gene Ther. 1997, 4, 773-782.
    • (1997) Gene Ther. , vol.4 , pp. 773-782
    • Baker, A.1    Saltik, M.2    Lehrmann, H.3    Killisch, I.4    Mautner, V.5    Lamm, G.6    Christofori, G.7    Cotton, M.8
  • 9
    • 0028725181 scopus 로고
    • Cultural and physiological factors affecting expression of recombinant proteins
    • Griffiths, J. B.; Racher, A. J. Cultural and physiological factors affecting expression of recombinant proteins. Cytotechnology 1994, 15, 3-9.
    • (1994) Cytotechnology , vol.15 , pp. 3-9
    • Griffiths, J.B.1    Racher, A.J.2
  • 10
    • 0032438794 scopus 로고    scopus 로고
    • Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions
    • Varani, J.; Piel, F.; Joseph, S.; Beals, T. F.; Hillegas, W. J. Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions. Cytotechnology 1998, 28, 101-109.
    • (1998) Cytotechnology , vol.28 , pp. 101-109
    • Varani, J.1    Piel, F.2    Joseph, S.3    Beals, T.F.4    Hillegas, W.J.5
  • 11
    • 0032402319 scopus 로고    scopus 로고
    • a(1, 3)-fucosyltransferase VII - Dependent synthesis of P- and E-selectin ligands on cultured T lymphoblasts
    • Knibbs, R. N.; Craig, R. A.; Maly, P.; Smith, P. L.; Wolber, F.; Faulkner, N. E.; Lowe, J. B.; Stoolman, L. M. a(1, 3)-fucosyltransferase VII - dependent synthesis of P- and E-selectin ligands on cultured T lymphoblasts. J. Immunol. 1998, 161, 6305-6315.
    • (1998) J. Immunol. , vol.161 , pp. 6305-6315
    • Knibbs, R.N.1    Craig, R.A.2    Maly, P.3    Smith, P.L.4    Wolber, F.5    Faulkner, N.E.6    Lowe, J.B.7    Stoolman, L.M.8
  • 12
    • 0024652453 scopus 로고
    • Substrate-dependent differences in production of extracellular matrix molecules by squamous carcinoma cells and diploid fibroblasts
    • Varani, J.; Fligiel, S. E. G.; Inman, D. R.; Helmreich, D. L.; Bendelow, M. J.; Hillegas, W. J. Substrate-dependent differences in production of extracellular matrix molecules by squamous carcinoma cells and diploid fibroblasts. Biotechnol. Bioeng. 1989, 33, 1235-1241.
    • (1989) Biotechnol. Bioeng. , vol.33 , pp. 1235-1241
    • Varani, J.1    Fligiel, S.E.G.2    Inman, D.R.3    Helmreich, D.L.4    Bendelow, M.J.5    Hillegas, W.J.6
  • 13
    • 0024534377 scopus 로고
    • Changes in integrin receptors on oncogenically transformed cells
    • Plantefaber, L. C.; Hynes, R. O. Changes in integrin receptors on oncogenically transformed cells. Cell 1989, 56, 281-298.
    • (1989) Cell , vol.56 , pp. 281-298
    • Plantefaber, L.C.1    Hynes, R.O.2
  • 14
    • 0022891814 scopus 로고
    • Cell growth on microcarriers: Comparison of proliferation and recovery from various substrates
    • Varani, J.; Bendelow, M. J.; Chun, J. H.; Hillegas, W. J. Cell growth on microcarriers: Comparison of proliferation and recovery from various substrates. J. Biol. Stand. 1986, 14, 331-336.
    • (1986) J. Biol. Stand. , vol.14 , pp. 331-336
    • Varani, J.1    Bendelow, M.J.2    Chun, J.H.3    Hillegas, W.J.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.