Purification and characterization of rice α-glucosidase, a key enzyme for alcohol fermentation of rice polish

Journal of Bioscience and Bioengineering

Volumn 95, Issue 1, 2003, Pages 106-108

  Iwata, Hiroshi ^a   Suzuki, Toshiaki ^a   Aramaki, Isao ^a  

^a NATIONAL RESEARCH INSTITUTE OF BREWING   (Japan)

Author keywords

Lactic acid; Nuka sake; Rice polish; glucosidase

Indexed keywords

BACTERIA; ENZYMES; FERMENTATION; GRAIN (AGRICULTURAL PRODUCT); MOLECULAR WEIGHT; ORGANIC ACIDS; PH EFFECTS; PURIFICATION;

BREWING;

BIOCHEMICAL ENGINEERING;

ALCOHOL; ALPHA GLUCOSIDASE; DNA; LACTIC ACID;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM CONTAMINATION; BREWING; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; FERMENTATION; MOLECULAR WEIGHT; RICE;

BACTERIA (MICROORGANISMS); ORYZA SATIVA;

EID: 0037272612     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.95.106     Document Type: Article

References (9)

1. Iwata, H., Nagano, T., Shiokawa, K., and Suzuki, A.: Development of a new alcoholic beverage made from uncooked rice bran. J. Brew. Soc. Jpn., 93, 139-147 (1998). (in Japanese)
2. Iwata, H., Suzuki, T., Takahashi, K., and Aramaki, I.: Critical importance of α-glucosidase in rice kernel for alcohol fermentation from rice polish. J. Biosci. Bioeng., 93, 296-302 (2002).
3. Bradford, M. M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-354 (1976).
4. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
5. Takahashi, N., Shimomura, T., and Chiba, S.: Studies on α-glucosidase in rice. Part I. Isolation and some properties of α-glucosidase I and α-glucosidase II. Agr. Biol. Chem., 35, 2015-2024 (1971).
6. Matsui, H., Ito, H., and Chiba, S.: Low molecular-activity α-glucosidase from ungerminated rice seed. Agr. Biol. Chem., 52, 1859-1860 (1988).
7. Frandsen, T. P., Lok, F., Migorodskaya, E., Roepstorff, P., and Svensson, B.: Purification, enzymatic characterization, and nucleotide sequence of a high-isoelectric-point α-glucosidase from barley malt. Plant Physiol., 123, 275-286 (2000).
8. Takahashi, M. and Shimomura, T.: Biochemical studies on α-glucosidase from buckwheat. Part II. Purification of buckwheat α-glucosidase and some properties on maltose and soluble starch. Agr. Biol. Chem., 32, 929-939 (1968).
9. Takahashi, M., Shimomura, T., and Chiba, S.: Biochemical studies on buckwheat α-glucosidase Part III. Transglucosilation action of the enzyme and isolation of reaction products. Agr. Biol. Chem., 33, 1399-1410 (1969).