Species-Specific Primer for Identification of Anopheles quadriannulatus sp. B (Diptera: Culicidae) from Ethiopia Using a Multiplex Polymerase Chain Reaction Assay

Journal of Medical Entomology

Volumn 40, Issue 1, 2003, Pages 112-115

  Fettene, Messay ^a,b   Temu, Emmanuel A ^a  

^a UNIVERSITY OF THE WITWATERSRAND   (South Africa)

^b JIMMA UNIVERSITY   (Ethiopia)

Author keywords

Anopheles gambiae; Anopheles quadriannulatus sp. B; Ethiopia; Pcr assay

Indexed keywords

ANOPHELES GAMBIAE; ANOPHELES QUADRIANNULATUS; ANOPHELES QUADRIANNULATUS B; CULICIDAE; DIPTERA; INSECTA;

PRIMER DNA;

ANIMAL; ANOPHELES; ARTICLE; CLASSIFICATION; ETHIOPIA; GENETICS; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SOUTH AFRICA; SPECIES DIFFERENCE;

ANIMALS; ANOPHELES; BASE SEQUENCE; DNA PRIMERS; ETHIOPIA; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, NUCLEIC ACID; SOUTH AFRICA; SPECIES SPECIFICITY;

EID: 0037271154     PISSN: 00222585     EISSN: None     Source Type: Journal    
DOI: 10.1603/0022-2585-40.1.112     Document Type: Article

References (9)

1. Collins, F. H., M. A. Mendez, P. C. Rasmussen, H. J. Mahaffey, N. J. Besansky, and V. Fennerty. 1987. A ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex. Am. J. Trop. Med. Hyg. 37: 37-41.
2. Corpet, F. 1988. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. 16: 10881-10890.
3. Fettene, M., L. L. Koekemoer, R. H. Hunt, and M. Coetzee. 2002. A polymerase chain reaction assay for identification of Anopheles quadriannulatus species B, a member of the Anopheles gambiae complex. Med. Vet. Entomol. 16: 214-217.
4. Gillies, M. T., and M. Coetzee. 1987. A supplement to the Anophelinae of Africa South of the Sahara. Publications of the South African Institute for Medical Research, No. 55, Johannesburg, South Africa.
5. Hunt, R. H., M. Coetzee, and M. Fettene. 1998. The Anopheles gambiae complex: a new species from Ethiopia. Trans. R. Soc. Trop. Med. Hyg. 92: 231-235.
6. Scott, J. A., W. G. Brogdon, and F. H. Collins. 1993. Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction. Am. J. Trop. Med. Hyg. 49: 520-529.
7. Townson, H., and A. W. Onapa. 1994. Identification by the rDNA-PCR of Anopheles bwambae, a geothermal spring species of the An. gambiae complex. Insect Mol. Biol. 3: 279-282.
8. Van Rensburg, A. J., R. H. Hunt, L. L. Koekemoer, M. Coetzee, C. J. Shiff, and J. Minjas. 1996. The polymerase chain reaction method as a tool for identifying members of the Anopheles gambiae complex (Diptera: Culicidae) in northeastern Tanzania. J. Am. Mosq. Control Assoc. 12: 271-274.
9. White, G. B. 1974. Anopheles gambiae complex and disease transmission in Africa. Trans. R. Soc. Trop. Med. Hyg. 68: 278-301.