Cloning, expression of the lectin-EGF domain of P-selectin, and preparation of its monoclonal antibody

Acta Biochimica et Biophysica Sinica

Volumn 35, Issue 2, 2003, Pages 172-176

  Zhou, Tong ^a   Song, Wei ^a   Wang, Feng ^a   Ni, Pei Hua ^a   Chen, Nan ^a   Zhang, Dong Qing ^b   Yu, Qi Wen ^b  

^a SHANGHAI SECOND MEDICAL UNIVERSITY   (China)

^b SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

Domains; Functions; Monoclonal antibody; P selectin; Prokaryotic expression

Indexed keywords

EPIDERMAL GROWTH FACTOR; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G2; IMMUNOGLOBULIN G3; IMMUNOGLOBULIN KAPPA CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; LECTIN; LIPOPOLYSACCHARIDE; MONOCLONAL ANTIBODY; NICKEL; NITRILOTRIACETIC ACID; PADGEM PROTEIN; UREA;

ARTICLE; BACTERIAL STRAIN; CELL ADHESION; CELL LINE; COLUMN CHROMATOGRAPHY; DNA VECTOR; DRUG EFFECT; DRUG SYNTHESIS; ELUTION; ENDOTHELIUM CELL; ESCHERICHIA COLI; FLUORESCENCE ACTIVATED CELL SORTING; GENE AMPLIFICATION; HYBRIDOMA; HYBRIDOMA CELL CULTURE; IMMUNOFLUORESCENCE; IN VITRO STUDY; INHIBITION KINETICS; MOLECULAR CLONING; NEUTROPHIL; NONHUMAN; PH; PROKARYOTE; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RECOMBINANT PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCREENING; STIMULATION; TECHNIQUE; THROMBOCYTE; THROMBOCYTE ADHESION;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; BINDING SITES; BLOOD PLATELETS; CELL ADHESION; CLONING, MOLECULAR; EPIDERMAL GROWTH FACTOR; FEMALE; GENE EXPRESSION; HUMANS; HYBRIDOMAS; LECTINS; MICE; MICE, INBRED BALB C; NEUTROPHILS; P-SELECTIN; TUMOR CELLS, CULTURED;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROKARYOTA;

EID: 0037261160     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Johnston GI, Cook RG, McEver RP. Cloning of GMP-140, a granule membrane protein of platelets and endothelium: Sequence similarity to proteins involved in cell adhesion and inflammation. Cell, 1989, 56(6): 1033-1044
2. Bevilacqua MP, Nelson RM. Selectins. J Clin Invest, 1993, 91(2): 379-387
3. Pendl GG, Robert C, Steinert M, Thanos R, Eytner R, Borges E, Wild MK et al. Immature mouse dendritic cells enter inflamed tissue, a process that requires E- and P-selectin, but not P-selectin glycoprotein ligand 1. Blood, 2002, 99(3): 946-956
4. Song W, Ni PH, Li X, Zhou T. Recent advances in the research of P-selectin domains and their function. Chinese Bulletin of Life Sciences, 2001, 13(2): 82-84
5. Li X, Zhou T, Hao CL, Dong DC, Cheng F, Chen SS. Significance of P-selectin expression in human glomerulonephritis. Chinese Medical Journal, 1997, 110(7): 512-514
6. Zhou T, Li X, Chen JL, Wu YL, Wang RN, Lou DX, Dong DC. Expession and Significance of adhesion molecule P-selectin in gastric and renal cell carcinomas. Natl Med J China, 1997, 77(7): 530-531
7. Zhou T, Li X, Wu P, Zhang DH, Zhang MJ, Chen N, Dong DC. Effect of anti-P-selectin monoclonal antibody on renal ischemia/reperfusion injury in rats. Chinese Medical Journal, 2000, 113(9): 790-793
8. Chen JL, Zhang YX, Chu YD, Zhou T, Xu HM, Cheng J, Li ML. Inhibition of human stomach cancer metastasis in vivo by anti-P-selectin monoclonal antibody. Natl Med J China, 1998, 78(6): 437-439
9. Wang L, Brown JR, Varki A, Esko JD. Heparin's anti-inflammatory effects require glucosamine 6-O-sulfation and are mediated by blockade of L- and P-selectins. J Clin Invest, 2002, 110(1): 127-136
10. Gibson RM, Kansas GS, Tedder TF, Furie B, Furie BC. Lectin and epidermal growth factor domains of P-selectin at physiologic density are the recognition unit for leukocyte binding. Blood, 1995, 85(1): 151-158
11. Navarro C, Furie BC, Furie B. Binding of the lectin-EGF domains of P-selectin to PSGL-1 on monocytes is required for upregulation of tissue factor and is independent of CD14. Blood, 1996, 88(Suppl): 653 A-658A[Abstract]