Quantitative assessment of chondrocyte viability after laser mediated reshaping: A novel application of flow cytometry

Lasers in Surgery and Medicine

Volumn 32, Issue 1, 2003, Pages 3-9

  Rasouli, Alexandre ^a   Sun, Chung Ho ^a   Basu, Reshmi ^a   Wong, Brian J F ^a,b,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c University of California at Irvine   (United States)

Author keywords

Cartilage; Chondrocytes; Flow cytometry; Laser; Live Dead assay; Nasal septum; Otolaryngology; Pig; Plastic surgery; Tissue shape change; Viability

Indexed keywords

FLUORESCENT DYE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BIOPHYSICS; CARTILAGE; CARTILAGE CELL; CELL CULTURE; CELL VIABILITY; CONTROLLED STUDY; FLOW CYTOMETRY; LASER; MECHANICAL STRESS; NEODYMIUM LASER; NONHUMAN; NOSE SEPTUM; OTORHINOLARYNGOLOGY; PLASTIC SURGERY; PRIORITY JOURNAL; SWINE; TISSUE STRUCTURE;

ANIMALS; CELL SURVIVAL; CHONDROCYTES; DISEASE MODELS, ANIMAL; DOSE-RESPONSE RELATIONSHIP, RADIATION; FLOW CYTOMETRY; NASAL SEPTUM; REPRODUCIBILITY OF RESULTS; STRESS, MECHANICAL; SWINE;

EID: 0037258423     PISSN: 01968092     EISSN: None     Source Type: Journal    
DOI: 10.1002/lsm.10142     Document Type: Article

References (16)

1. Helidonis E, Sobol EN, Kavvalos G, Bizakis J, Christodoulou P, velegrakis G, Segas J, Bagratashvili VV. Laser shaping of composite cartilage grafts. Am J Otolaryngol 1993;14:410-412.
2. Sobol E, Sviridov A, Omeltchenko A, Bagratashvili V, Kitai M, Harding SE, et al. Laser reshaping of cartilage. Biotechnol Genet Eng Rev 2000;17:539-564.
3. Wang Z, Pankratov MM, Perrault DF, Shapshay SM. Endoscopic laser-assisted reshaping of collapsed tracheal cartilage: A laboratory study. Ann Otol Rhinol Laryngol 1996;105:176-181.
4. Wong BJF, Milner TE, Kim HK, Nelson JS, Sobol EN. Stress relaxation of porcine septal cartilage during Nd:YAG (λ = 1.32 mm) laser irradiation: Mechanical, optical, and thermal responses. J Biomed Opt 1998;3:409-414.
5. Wong BJF, Milner TE, Kim HK, Telenkov S, Chew CF, Sobol EN, et al. Characterization of temperature dependent biophysical properties during laser mediated cartilage reshaping. IEEE J Selected Top Quantum Electron 1999;5:1095-1102.
6. Wong BJF, Milner TE, Anvari B, Sviridov A, Omel'chenko A, Bagratashvili VV, et al. Measurement of radiometric surface temperature and integrated back-scattered light intensity during feedback controlled laser-assisted cartilage reshaping. Lasers Surg Med 1998;13:66-72.
7. Sviridov A, Sobol EN, Jones NS, Lowe J. Effect of holmium laser radiation on stress, temperature, and structure alterations in cartilage. Lasers Med Sci 1998;13:73-77.
8. Wong BJF, Milner TE, Harrington A, Ro J, Dao X, Sobol EN, et al. Feedback controlled laser mediated cartilage reshaping. Arch Facial Plast Surg 1999;1:282-287.
9. Wong BJF, Milner TE, Kim HK, Chao K, Sun C-H, Sobol EN, et al. Proteoglycan synthesis in porcine nasal cartilage grafts following Nd:YAG (λ = 1.32 mm) laser mediated reshaping. Photochem Photobiol 2000;71:218-224.
10. Wong BJF, Chao K, Kim HK, Chu EA, Dao X, Gaon M, et al. The porcine and lagomorph septal cartilages: Models for tissue engineering and morphologic cartilage research. Am J Rhinol 2001;15:109-116.
11. Youn J-I, Telenkov SA, Kim E, Bhavaraju NC, Wong BJF, Valvano JW, et al. Optical and thermal properties of nasal septal cartilage. Lasers Surg Med 2000;27:119-128.
12. Anvari B, Milner TE, Tanenbaum BS, Kimel S, Svaasand LO, Nelson JS. Selective cooling of biological tissues: Application for thermally mediated therapeutic procedures. Phys Med Biol 1995;40:241-252.
13. Karamzadeh AM, Rasouli A, Tanenbaum BS, Milner TE, Nelson JS, Wong BJF. Laser-mediated cartlage reshaping with feedback-controlled cryogen spray cooling: Biophysical properties and viability. Lasers Surg Med 2001;28:1-10.
14. Keefe M, Rasouli A, Telenkov S, Karamzadeh AM, Milner TE, Crumley RL, et al. Radiofrequency cartilage reshaping: Efficacy, biophysical measurements, and tissue viability. Arch Facial Plast Surg 2002; in press.
15. Bujia J, Kremer D, Sudhoff H, Viviente E, Sprekelsen C, Wilmes E. Determination of viability of cryopreserved cartilage grafts. Eur Arch Otorhinolaryngol 1995;252:30-34.
16. Darzynkiewicz Z, Li X, Gong J. Assays of cell viability: Discrimination of cells dying by apoptosis. Methods Cell Biol 1994;41:15-38.