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note
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No sponge-derived endoplasmic reticulum (ER) inhibitors have been discovered to date. An assay, designed by Dr. T. A. Rapoport, at the Institute of Chemistry and Cell Biology, Howard Hughes Medical Institute Research Laboratories, Harvard Medical School, employed Xenopus frog membranes and cytosol that stimulates the formation of an ER network. He evaluated more than 200 Indo-Pacific sponge extracts in the ER assay and found four extracts including that of Collect. No. 95653 to inhibit the formation of a reticular network by the formation of aggregates.
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20
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12244258823
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note
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The unstable "methanol adduct" referred to in ref 5b, formed by cooling 2 with methanol, is most likely the 1,2 or 1,6 methanol addition product.
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21
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0000260530
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For additional details about this assay see: Varoglu, M.; Corbett, T. H.; Valeriote, F. A.; Crews, P. J. Org. Chem. 1997, 62, 7078-7079.
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