Processing sputum specimens in a refrigerated centrifuge does not increase the rate of isolation of Mycobacterium tuberculosis

Journal of Clinical Microbiology

Volumn 41, Issue 1, 2003, Pages 469-471

  Selvakumar, N ^a   Govindan, Dakshayani ^a   Chandu, Naik Ashok ^a   Frieden, Thomas R ^b   Narayanan, P R ^a  

^a INDIAN COUNCIL OF MEDICAL RESEARCH   (India)

^b WORLD HEALTH ORGANIZATION   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CETYLPYRIDINIUM SALT;

ANALYTIC METHOD; ARTICLE; BACTERIUM CULTURE; BACTERIUM ISOLATION; CENTRIFUGATION; CONTROLLED STUDY; FREEZING; HUMAN; INTERMETHOD COMPARISON; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; SPUTUM CULTURE;

CENTRIFUGATION; HUMANS; MICROBIOLOGICAL TECHNIQUES; MYCOBACTERIUM TUBERCULOSIS; REFRIGERATION; SPUTUM;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; UNCULTURED ACTINOMYCETE;

EID: 0037232148     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.41.1.469-471.2003     Document Type: Article

References (9)

1. Abee, T. M., and J. A. Wouters. 1999. Microbial stress response in minimal processing. Int. J. Food Microbiol. 50:65-91.
2. Canetti, G., N. Rist, and J. Grosset. 1964. Primary drug resistance in tuberculosis. Am. Rev. Tuberc. Pulm. Dis. 90:792-799.
3. Central TB Division, Directorate General of Health Services, Ministry of Health and Family Welfare, New Delhi, India. 1998. Revised national tuberculosis control programme. Manual for laboratory technicians. [Online.] http://www.tbcindia.orgs/LAB-MANUAL.pdf
4. Kent, P. T., and G. P. Kubica. 1985. Public health mycobacteriology: a guide for the level III laboratory, p. 159-184. U.S. Department of Health and Human Services, Centers for Disease Control, Atlanta, Ga.
5. Paramasivan, C. N., A. S. L. Narayana, R. Prabhakar, M. S. Rajagopal, P. R. Somasundaram, and S. P. Tripathy. 1983. Effect of storage of sputum specimens at room temperature on smear and culture results. Tubercle 64:119-124.
6. Selvakumar, N., V. Kumar, A. S. L. Narayana, D. Surayanarayanan, K. C. Umapathy, C. N. Paramasivan, and R. Prabhakar. 1993 Use of cetylpyridinium chloride for storage of sputum specimens and isolation of M. tuberculosis. Indian J. Tuberc. 40:95-97.
7. Selvakumar, N., V. Kumar, P. G. Gopi, K. V. Venkataramu, M. Datta, C. N. Paramasivan, and R. Prabhakar. 1995. Isolation of tubercle bacilli from sputum samples of patients in the field studies by the cetylpyridinium chloride-sodium chloride and sodium hydroxide methods. Indian J. Med. Res. 102:149-151.
8. Smithwick, R. W., C. B. Stratigoes, and H. L. David. 1975. Use of cetylpyridinium chloride for the decontamination of sputum specimens that are transported to the laboratory for the isolation of Mycobacterium tuberculosis. J. Clin. Microbiol. 1:411-413.
9. Tazir, M., H. L. David, and F. Boulahbad. 1979. Evaluation of the chloride and bromide salts of cetylpyrinidium for the transportation of sputum in tuberculosis bacteriology. Tubercle 60:31-36.