Synthesis of GDP-mannose and mannosylglycerate from labeled mannose by genetically engineered Escherichia coli without loss of specific isotopic enrichment

Applied and Environmental Microbiology

Volumn 69, Issue 1, 2003, Pages 233-240

  Sampaio, Maria Manuel ^a   Santos, Helena ^a   Boos, Winfried ^b  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CATALYSIS; CELLS; CHROMATOGRAPHY; ENZYMES; GENES; ISOTOPES;

COLANIC ACIDS;

MICROBIOLOGY;

COLANIC ACID; FRUCTOSE 6 PHOSPHATE; GLYCERIC ACID; GUANOSINE DIPHOSPHATE MANNOSE; MANNOSE; MANNOSE 1 PHOSPHATE GUANYLYLTRANSFERASE; MANNOSE 6 PHOSPHATE; MANNOSE PHOSPHATE ISOMERASE; MANNOSYLGLYCERATE; MANNOSYLGLYCERATE SYNTHASE; PHOSPHOMANNOMUTASE; PHOSPHOTRANSFERASE; POLYMER; SUGAR ACID; UNCLASSIFIED DRUG;

BACTERIUM; PLASMID;

ARTICLE; BACTERIAL STRAIN; BACTERIUM; CARBOHYDRATE METABOLISM; CATALYSIS; CPS GENE; ENRICHMENT CULTURE; ESCHERICHIA COLI; GENE; GENE CLUSTER; GENE DELETION; GENETIC CODE; GENETIC ENGINEERING; ISOTOPE LABELING; MANA GENE; MSG GENE; MUTANT; NONHUMAN; PAPER CHROMATOGRAPHY; PLASMID; RHODOTHERMUS MARINUS; SUGAR TRANSPORT; SYNTHESIS; WATER IMMERSION;

CARBON RADIOISOTOPES; ESCHERICHIA COLI; GENE DELETION; GENETIC ENGINEERING; GLYCERIC ACIDS; GUANOSINE DIPHOSPHATE MANNOSE; MANNOSE; MANNOSE-6-PHOSPHATE ISOMERASE; MANNOSYLTRANSFERASES; NUCLEOTIDYLTRANSFERASES; PHOSPHOTRANSFERASES (PHOSPHOMUTASES); PLASMIDS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; RHODOTHERMUS MARINUS;

EID: 0037229829     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.1.233-240.2003     Document Type: Article

References (25)

1. Amann, E., B. Ochs, and K. J. Abel. 1988. Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli. Gene 69:301-315.
2. Andrianopoulos, K., L. Wang, and P. R. Reeves. 1998. Identification of the fucose synthetase gene in the colanic acid gene cluster of Escherichia coli K-12. J. Bacteriol. 180:998-1001.
3. Borges, N., A. Ramos, N. D. H. Raven, R. J. Sharp, and H. Santos. 2002. Comparative study of the thermostabilizing properties of mannosylglycerate and other compatible solutes on model enzymes. Extremophiles 6:209-216.
4. Bouveng, H., B. Lindberg, and B. Wickberg. 1955. Low-molecular carbohydrates in algae. Acta Chem. Scand. 9:807-809.
5. Casadaban, M. J. 1976. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J. Mol. Biol. 104:541-555.
6. Chang, A. C., and S. N. Cohen. 1978. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134:1141-1156.
7. Empadinhas, N., J. D. Marugg, N. Borges, H. Santos, and M. S. da Costa. 2001. Pathway for the synthesis of mannosylglycerate in the hyperthermophilic archaeon Pyrococcus horikoshii. Biochemical and genetic characterization of key enzymes. J. Biol. Chem, 276:43580-43588.
8. Giaever, H. M., O. B. Styrvold, I. Kaasen, and A. R. Strom. 1988. Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli. J. Bacteriol. 170:2841-2849.
9. Gottesman, S., and V. Stout. 1991. Regulation of capsular polysaccharide synthesis in Escherichia coli K12. Mol. Microbiol. 5:1599-1606.
10. Horlacher, R., and W. Boos. 1997. Characterization of TreR, the major regulator of the Escherichia coli trehalose system. J. Biol. Chem. 272:13026-13032.
11. Jacin, H., and A. R. Mishkin. 1965. Separation of carbohydrates on borate-impregnated silica gel G plates. J. Chromatogr. 18:170-173.
12. Martins, L. O., N. Empadinhas, J. D. Marugg, C. Miguel, C. Ferreira, M. S. da Costa, and H. Santos. 1999. Biosynthesis of mannosylglycerate in the thermophilic bacterium Rhodothermus marinus. Biochemical and genetic characterization of a mannosylglycerate synthase. J. Biol. Chem. 274:35407-35414.
13. McLaggan, D., M. A. Jones, G. Gouesbet, N. Levina, S. Lindey, W. Epstein, and I. R. Booth. 2002. Analysis of the kefA2 mutation suggests that KefA is a cation-specific channel involved in osmotic adaptation in Escherichia coli. Mol. Microbiol. 43:521-536.
14. Meissner, P. S., W. P. Sisk, and M. L. Berman. 1987. Bacteriophage lambda cloning system for the construction of directional cDNA libraries. Proc. Natl. Acad. Sci. USA 84:4171-4175.
15. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory. Cold Spring Harbor. N.Y.
16. Nuoffer, C., B. Zanolari, and B. Erni. 1988. Glucose permease of Escherichia coli. The effect of cysteine to serine mutations on the function, stability, and regulation of transport and phosphorylation. J. Biol. Chem. 263:6647-6655.
17. Plumbridge, J. 1998. Expression of ptsG, the gene for the major glucose PTS transporter in Escherichia coli, is repressed by Mlc and induced by growth on glucose, Mol. Microbiol. 29:1053-1063.
18. Postma, P. W., J. W. Lengeler, and G. R. Jacobson. 1993. Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. Microbiol. Rev. 57:543-594.
19. Ramos, A., N. Raven, R. Sharp, S. Bartolucci, M. Rossi, R. Cannio, J. Lebbink, J. Van Der Oost, W. De Vos, and H. Santos. 1997. Stabilization of enzymes against thermal stress and freeze-drying by mannosylglycerate. Appl. Environ. Microbiol. 63:4020-4025.
20. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor. N.Y.
21. Santos, H., and M. S. da Costa. 2001. Organic solutes from thermophiles and hyperthermophiles. Methods Enzymol. 334:302-315.
22. Schlegel, A., O. Danot, E. Richet, T. Ferenci, and W. Boos. 2002. The N terminus of the Eschetichia coli transcription activator MalT is the domain of interaction with MalY. J. Bacteriol. 184:3069-3077.
23. Silva, Z., N. Borges, L. O. Martins, R. Wait, M. S. da Costa, and H. Santos. 1999. Combined effect of the growth temperature and salinity of the medium on the accumulation of compatible solutes by Rhodothermus marinus and Rhodothermus obamensis. Extremophiles 3:163-172.
24. Singer, M., T. A. Baker, G. Schnitzler, S. M. Deischel, M. Goel, W. Dove, K. J. Jaacks, A. D. Grossman, J. W. Erickson, and C. A. Gross. 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53:1-24.
25. Stevenson, G., K. Andrianopoulos, M. Hobbs, and P. R. Reeves. 1996. Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid. J. Bacteriol. 178:4885-4893.