Separation of DNA by capillary electrophoresis in uncoated silica columns using hydroxypropylmethyl cellulose as the sieving matrix

Analytical Letters

Volumn 36, Issue 2, 2003, Pages 451-463

  Villareal, V ^a   Zhang, Y ^a   Zurita, C ^a   Moran, J ^a   Silva, I ^a   Gomez, F A ^a  

^a CALIFORNIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOPOLYMER; DNA FRAGMENT; HYDROXYPROPYLMETHYLCELLULOSE; SILICON DIOXIDE;

ARTICLE; CAPILLARY ELECTROPHORESIS; DNA DETERMINATION; DNA STRUCTURE; ELECTROPHORESIS; ESCHERICHIA COLI; PLASMID; PROTEIN ANALYSIS;

ESCHERICHIA COLI;

EID: 0037225945     PISSN: 00032719     EISSN: None     Source Type: Journal    
DOI: 10.1081/AL-120017702     Document Type: Article

References (29)

1. Beale, S.C. Capillary electrophoresis. Anal. Chem. 1998, 70, 279R-300R.
2. Strege, A.; Lagu, A.L. Capillary electrophoresis of biotechnology-derived proteins. Electrophoresis 1997, 18, 2343-2352.
3. Zhang, Y.; El-Maghrabi, R.; Gomez, F.A. Use of capillary electrophoresis and indirect detection to quantitate in-capillary enzyme-catalyzed microreactions. Analyst 2000, 125, 685-688.
4. Manabe, T.; Chen, N.; Terabe, S.; Yohda, M.; Endo, I. Effects of linear polyacrylamide concentrations and applied voltages on the separation of oligonucleotides and DNA sequencing fragments by capillary electrophoresis. Anal. Chem. 1994, 66, 4243-4252.
5. Voss, K.O.; Roos, K.P.; Nonay, R.L.; Dovichi, N.J. Combating PCR bias in bisulfite-based cytosine methylation analysis. Betaine-modified cytosine deamination PCR. Anal. Chem. 1998, 70, 3818-3823.
6. Stebbins, M.A.; Hoyt, A.M.; Sepaniak, M.J.; Hurlburt, B.K. Design and optimization of a capillary electrophoretic mobility shift assay involving TRP repressor-DNA complexes. J. Chromatogr. B 1996, 683, 77-84.
7. Zhou, H.; Miller, A.W.; Sosic, Z.; Buchholz, B.; Barron, A.E.; Kotler, L.; Karger, B.L. DNA sequencing up to 1300 bases in two hours by capillary electrophoresis with mixed replaceable linear polyacrylamide solution. Anal. Chem. 2000, 72, 1045-1052.
8. Xian, J.; Harrington, M.G.; Davidson, E.H. DNA-protein binding assays from a single sea urchin egg: a high-sensitivity capillary electrophoresis method. Proc. Natl. Acad. Sci. USA 1996, 93, 86-90.
9. Raucci, G.; Maggi, C.A.; Parente, D. Capillary electrophoresis of supercoiled DNA molecules: parameters governing the resolution of topoisomers and their separation from open forms. Anal. Chem. 2000, 72, 821-826.
10. Owens, C.V.; Davidson, Y.Y.; Kar, S.; Soper, S.A. High-resolution separation of DNA restriction fragments using capillary electrophoresis with near-IR, diode-based, laser-induced fluorescence detection. Anal. Chem. 1997, 69, 1256-1261.
11. Zhu, H.; Clark, S.M.; Benson, S.C.; Rye, H.S.; Glazer, A.N.; Mathies, R.A. High-sensitivity capillary electrophoresis of double-stranded DNA fragments using monomeric and dimeric fluorescent intercalating dyes. Anal. Chem. 1994, 66, 1941-1948.
12. Shi, X.; Hammond, R.W.; Morris, M.D. DNA conformational dynamics in polymer solutions above and below the entanglement limit. Anal. Chem. 1995, 67, 1132-1138.
13. Figeys, D.; Arriaga, E.; Renbor, A.; Dovichi, N.J. Use of the fluorescent intercalating dyes POPO-3, YOYO-3 and YOYO-1 for ultrasensitive detection of double-stranded DNA separated by capillary electrophoresis with hydroxypropylmethyl cellulose and non-cross-linked polyacrylamide. J. Chromatogr. A 1994, 669, 205-216.
14. Fung, E.N.; Yeung, E.S. High-speed DNA sequencing by using mixed poly(ethylene oxide) solutions in uncoated capillary columns. Anal. Chem. 1995, 67, 1913-1919.
15. Chang, H.-T.; Yeung, E.S. Poly(ethylene oxide) for high-resolution and high-speed separation of DNA by capillary electrophoresis. J. Chromatogr. A 1995, 669, 113-123.
16. Huang, X.C.; Stuart, S.G.; Bente, P.F.; Brennan, T.M. Capillary gel electrophoresis of single-stranded DNA fragments with UV detection. J. Chromatogr. 1992, 600, 289-295.
17. Hebenbrock, K.; Williams, P.M.; Karger, B.L. Single strand conformational polymorphism using capillary electrophoresis with two-dye laser-induced fluorescence detection. Electrophoresis 1995, 16, 1429-1436.
18. Ganguly, A.; Prockop, D.J. Detection of mismatched bases in double stranded DNA by gel electrophoresis. Electrophoresis 1995, 16, 1830-1835.
19. Foulds, G.J.; Etzkorn, F.A.J. DNA-binding affinities of MyoD and E47 homo-and hetero-dimers by capillary electrophoresis mobility shift assay. Chromatogr. A 1999, 862, 231-236.
20. Esaka, Y.; Yamaguchi, Y.; Kano, K.; Goto, M.; Haraguchi, H.; Takahashi, J. Separation of hydrogen-bonding donors in capillary electrophoresis using polyethers as matrix. Anal. Chem. 1994, 66, 2441-2445.
21. Ng, M.; Blaschke, T.F.; Arias, A.A.; Zare, R.N. Analysis of free intracellular nucleotides using high-performance capillary electrophoresis. Anal. Chem. 1992, 64, 1682-1684.
22. Nguyen, A.-L.; Luong, J.H.T., Masson, C. Determination of nucleotides in fish tissues using capillary electrophoresis. Anal. Chem. 1990, 62, 2490-2493.
23. Tadey, T.; Purdy, W.C. Capillary electrophoretic separation of nucleotide isomers via complexation with cyclodextrin and borate. J. Chromatogr. B 1994, 657, 365-372.
24. Shihabi, Z.K. Field amplified injection in the presence of salts for capillary electrophoresis. J. Chromtogr. A 1999, 853, 3-9.
25. Chen, H.S.; Chang, H.-T. Stepwise capillary electrophoretic separation of dna fragments using poly(ethylene oxide) solutions in the presence of electroosmotic flow. J. Chromtogr. A 1999, 853, 337-347.
26. Fung, E.N.; Pang, H.; Yeung, E.S. Fast DNA separations using poly(ethylene oxide) in non-denaturing medium with temperature programming. J. Chromatogr. A 1998, 806, 157-164.
27. de Carmejane, O.; Schwinefus, J.J.; Wang, S.-C.; Morris, M.D. Electrophoretic separation of linear and supercoiled DNA in uncoated capillaries. J. Chromatogr. A 1999, 849, 267-276.
28. Bode, H.J. The use of liquid polyacrylamide in electrophoresis. II. relationship between gel viscosity and molecular sieiving. Anal. Biochem. 1977, 83, 364-371.
29. Cantor, C.R.; Smith, C.L.; Mathew, M.K. Pulsed-field gel electrophoresis of very large DNA molecules. Annu. Rev. Biophys. Chem. 1988, 17, 287-304.