Identification of a helix-turn-helix motif of Bacillus thermoglucosidasius HrcA essential for binding to the CIRCE element and thermostability of the HrcA-CIRCE complex, indicating a role as a thermosensor

Journal of Bacteriology

Volumn 185, Issue 1, 2003, Pages 381-385

  Hitomi, Masafumi ^a   Nishimura, Hiroshi ^b   Tsujimoto, Yoshiyuki ^a   Matsui, Hiroshi ^a   Watanabe, Kunihiko ^a  

^a KYOTO PREFECTURAL UNIVERSITY   (Japan)

^b KYOTO PREFECTURAL UNIVERSITY OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CHAPERONE; DNA BINDING PROTEIN; HEAT SHOCK PROTEIN; HELIX LOOP HELIX PROTEIN; PROTEIN HRCA; REPRESSOR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; BACILLUS THERMOGLUCOSIDASIUS; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIUM; DNA BINDING; HEAT SHOCK RESPONSE; NONHUMAN; OPERON; PRIORITY JOURNAL; THERMORECEPTOR; THERMOREGULATION; THERMOSTABILITY;

AMINO ACID SEQUENCE; BACILLUS; BACTERIAL PROTEINS; CHAPERONINS; DNA, BACTERIAL; DNA-BINDING PROTEINS; ESCHERICHIA COLI PROTEINS; HEAT-SHOCK PROTEINS; HEAT-SHOCK RESPONSE; HELIX-TURN-HELIX MOTIFS; HSP70 HEAT-SHOCK PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN DENATURATION; REPETITIVE SEQUENCES, NUCLEIC ACID; REPRESSOR PROTEINS; TEMPERATURE;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); GEOBACILLUS THERMOGLUCOSIDASIUS; POSIBACTERIA;

EID: 0037216733     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.1.381-385.2003     Document Type: Article

References (34)

1. Ahmad, S., A. Selvapandiyan, and R. K. Bhantnagar. 1999. A protein-based phylogenetic tree for gram-positive bacteria derived from hrcA, a unique heat-shock regulatory gene. Int. J. Syst. Bacteriol. 49:1387-1394.
2. Baba, T., F. Takeuchi, M. Kuroda, H. Yuzawa, K. Aoki, A. Oguchi, Y. Nagai, N. Iwama, K. Asano, T. Naimi, H. Kuroda, L. Cui, K. Yamamoto, and K. Hiramatsu. 2002. Genome and virulence determinants of high virulence community-acquired MRSA. Lancet 359:1819-1827.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
4. Christodoulou, E., and C. E. Vorgias. 2002. The thermostability of DNA-binding protein HU from mesophilic, thermophilic, and extreme thermophilic bacteria. Extremophiles 6:21-31.
5. Claus, D., and R. C. W. Berkeley. 1986. Genus Bacillus Cohn 1872, p. 1105-1139. In P. H. A. Sneath, N. S. Mair, M. E. Sharpe, and J. G, Holt (ed.), Bergey's manual of systematic bacteriology, vol. 2. Williams & Wilkins, Baltimore, Md.
6. Cuff, J. A., M. E. Clamp, A. S. Siddiqui, M. Finlay, and G. J. Barton. 1998. JPred: A consensus secondary structure prediction server. Bioinformatics 14:892-893.
7. Frishman, D., and P. Argos. 1996. Incorporation of non-local interactions in protein secondary structure prediction from the amino acid sequence. Protein Eng. 9:133-142.
8. Gibrat, J. F., J. Garnier, and B. Robson. 1987. Further developments of protein secondary structure prediction using information theory. New parameters and consideration of residue pairs. J. Mol. Biol. 198:425-443.
9. Hecker, M., W. Schumann, and U. Völker. 1996. Heat-shock and general stress response in Bacillus subtilis. Mol. Microbiol. 19:417-428.
10. Huffman, J. L., and R. G. Brennan. 2002. Prokaryotic transcription regulators: More than just the helix-turn-helix motif. Curr. Opin. Struct. Biol. 12:98-106.
11. Karaivanova, I. M., P. Wiegel, M. Takahashi, C. Fort, A. Versavaud, G. V. Duyne, D. Charlier, J.-N. Hallet, N. Glansdorff, and V. Sakanyan. 1999. Mutation analysis of the thermostable arginine repressor from Bacillus stearothermophilus: Dissecting residues involved in DNA binding properties. J. Mol. Biol. 291:843-855.
12. King, R. D., and M. J. Sternberg. 1996. Identification and application of the concepts important for accurate and reliable protein secondary structure prediction. Protein Sci. 5:2298-2310.
13. Lu, Z., and E. C. Lin. 1989. The nucleotide sequence of Escherichia coli genes for L-fucose dissimilation. Nucleic Acids Res. 17:4883-4884.
14. Mogk,. A., A. Völker, S. Engelmann, M. Hecker, W. Schumann, and U. Völker. 1998. Nonnative proteins induce expression of the Bacillus subtilis CIRCE regulon. J. Bacteriol. 180:2895-2900.
15. Mogk, A., G. Homuth, C. Scholz, L. Kim, F. X. Schmid, and W. Schumann. 1997. The GroE chaperonin machine is a major modulator of the CIRCE heat shock regulon of Bacillus subtilis. EMBO J. 16:4579-4590.
16. Mogk, A., and W. Schumann. 1997. Cloning and sequencing of the hrcA gene of Bacillus stearothermophilus. Gene 194:133-136.
17. Narberhaus, F. 1999. Negative regulation of bacterial heat shock genes. Mol. Microbiol. 31:1-8.
18. Nölling, J., G. Breton, M. V. Omelchenko, K. S. Makarova, Q. Zeng, R. Gibson, H. M. Lee, J. Dubois, D. Qiu, J. Hitti, GTC Sequencing Center Production, Finishing, and Bioinformatics Teams, Y. I. Wolf, R. L. Tatusov, F. Sabathe, L. Doucette-Stamm, P. Soucaille, M. J. Daly, G. N. Bennett, E. V. Koonin, and D. R. Smith. 2001. Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum. J. Bacteriol. 183:4823-4838.
19. Reischl, S., T. Wiegert, and W. Schumann. 2002. Isolation and analysis of mutant alleles of the Bacillus subtilis HrcA repressor with reduced dependency on GroE function. J. Biol. Chem. 277:32659-32667. (First published 24 June 2002; 10.1074/jbc.M201372200.)
20. Rost, B., and C. Sander. 1993. Prediction of protein secondary structure at better than 70% accuracy. J. Mol. Biol. 232:584-599.
21. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
22. Schulz, A., and W. Schumann. 1996. hrcA, the first gene of the Bacillus stubtilis dnaK operon encodes a negative regulator of class I heat shock genes. J. Bacteriol. 178:1088-1093.
23. Servant, P., C. Grandvalet, and P. Mazodier. 2000. The RheA repressor is the thermosensor of the HSP18 heat shock response in Streptomyces albus. Proc. Natl. Acad. Sci. USA 97:3538-3543.
24. Song, H., H. Wang, D. Gigot, D. Dimova, V. Sakanyan, N. Glansdorff, and D. Charlier. 2002. Transcription regulation in thermophilic bacteria: High resolution contact probing of Bacillus stearothermophilus and Thermotoga neapolitana arginine repressor-operator interactions. J. Mol. Biol. 315:255-274.
25. Suzuki, Y., T. Kishigami, K. Inoue, Y. Mizouchi, N. Eto, M. Takagi, and S. Abe. 1983. Bacillus thermoglucosidasius sp. nov., a new species of obligately thermophilic bacilli. Syst. Appl. Microbiol. 4:487-495.
26. Takami, H., K. Nakasone, Y. Takaki, G. Maeno, R. Sasaki, N. Masui, F. Fuji, C. Hirama, Y. Nakamura, N. Ogasawara, S. Kuhara, and K. Horikoshi. 2000. Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis. Nucleic Acids Res. 28:4317-4331.
27. Watanabe, K., K. Chishiro, K. Kitamura, and Y. Suzuki. 1991. Proline residues responsible for thermostability occur with high frequency in the loop regions of an extremely thermostable oligo-1,6-glucosidase from Bacillus thermoglucosidasius KP1006. J. Biol. Chem, 266:24287-24294.
28. Watanabe, K., and Y. Suzuki. 1998. Protein thermostabilization by proline substitutions. J. Mol. Catal. B 4:167-180.
29. Watanabe, K., T. Yamamoto, and Y. Suzuki. 2001. Renaturation of Bacillus thermoglucosidasius HrcA repressor by DNA and thermostability of HrcA-DNA complex in vitro. J. Bacteriol. 183:155-161.
30. Wintjens, R., and M. Rooman. 1996. Structural classification of HTH DNA-binding domains and protein-DNA interaction modes. J. Mol. Biol. 262:294-313.
31. Yamada, M., and M. H. J. Saier. 1988. Positive and negative regulators for glucitol (gut) operon expression in Escherichia coli. J. Mol. Biol. 203:569-583.
32. Yoshida, K., S. Saeki, M. Fujimura, Y. Miwa, and Y. Fujita. 1995. Cloning and sequencing of a 36-kb region of the Bacillus subtilis genome between the gnt and iol operons. DNA Res. 2:61-69.
33. Yuan, G., and S.-L. Wong. 1995. Isolation and characterization of Bacillus subtilis groE regulatory mutants: Evidence for orf39 in the dnaK operon as a repressor gene in regulating the expression of both groE and dnaK. J. Bacteriol. 177:6462-6468.
34. Zeng, G., S. Ye, and T. J. Larson. 1996. Repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K-12: Primary structure and identification of the DNA-binding domain. J. Bacteriol. 178:7080-7089.