PX domain takes shape

Current Opinion in Hematology

Volumn 10, Issue 1, 2003, Pages 2-7

  Wientjes, Frans B ^a   Segal, Anthony W ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

OXIDOREDUCTASE; PHAGOCYTE OXIDASE; PHOSPHATIDYLINOSITIDE; PHOSPHATIDYLINOSITOL 3 PHOSPHATE; PHOSPHATIDYLINOSITOL 3,4 BISPHOSPHATE; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE; PHOSPHATIDYLINOSITOL KINASE; POLYPHOSPHOINOSITIDE; PROTEIN TYROSINE KINASE; UNCLASSIFIED DRUG;

NEUTROPHIL; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN MOTIF; PROTEIN TARGETING; REVIEW; SIGNAL TRANSDUCTION; YEAST;

ANIMALS; BINDING SITES; HOMEODOMAIN PROTEINS; HUMANS; PHOSPHOLIPIDS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SRC HOMOLOGY DOMAINS; SUBSTRATE SPECIFICITY;

EID: 0037214463     PISSN: 10656251     EISSN: None     Source Type: Journal    
DOI: 10.1097/00062752-200301000-00002     Document Type: Review

References (30)

1. Xu Y, Seet LF, Hanson B, et al.: The Phox homology (PX) domain, a new player in phosphoinositide signaling. Biochem J 2001, 360:513-530.
2. Cullen PJ, Cozier GE, Banting G, et al.: Modular phosphoinositide-binding domains: Their role in signaling and membrane trafficking. Curr Biol 2001, 11:R882-R893.
3. Ponting CP: Novel domains in NADPH oxidase subunits, sorting nexins, and Ptdlns 3-kinases: Binding partners of SH3 domains? Protein Sci 1996, 5:2353-2357.
4. Noack D, Rae J, Cross AR, et al.: Autosomal recessive chronic granulomatous disease caused by defects in NCF-1, the gene encoding the phagocyte p47-phox: Mutations not arising in the NCF-1 pseudogenes. Blood 2001, 97:305-311.
5. phox-GFP fusion protein that was dependent upon PI 3-kinase activity and an intact PX domain.
6. Xu J, Liu D, Gill G, et al.: Regulation of cytokine-independent survival kinase (CISK) by the Phox homology domain and phosphoinositides. J Cell Biol 2001, 154:699-705.
7. Cheerer ML, Sato TK, de Beer T, et al.: Phox domain interaction with Ptdlns(3)P targets the Vam7 t-SNARE to vacuole membranes. Nat Cell Biol 2001, 3:613-618.
8. phox. This excellent study gives the overall structure of the PX domain and describes in detail how the phosphoinositide PI(3)P fits in its binding pocket and the amino acids interacting with it.
9. phox demonstrated, suggesting that PI 3-kinase enriches PX domain proteins on membrane.
10. Zhan Y, Virbasius JV, Song X, et al.: The p40phox and p47phox PX domains of NADPH oxidase target cell membranes via direct and indirect recruitment by phosphoinositides. J Biol Chem 2002, 277:4512-4518.
11. Yu JW, Lemmon MA: All phox homology (PX) domains from Saccharomyces cerevisiae specifically recognize phosphatidylinositol 3-phosphate. J Biol Chem 2001, 276:44179-44184. The authors investigated the PI-binding properties of all 15 yeast PX domain proteins. They were all shown to bind PI(3)P. The interesting observation was made that the yeast PX domains fall into two categories with strongly differing affinities for this phosphoinositide.
12. Zhong Q, Lazar CS, Tronchere H, et al.: Endosomal localization and function of sorting nexin 1. Proc Natl Acad Sci U S A 2002, 99:6767-6772. This is a detailed study of SNX1 describing its subcellular distribution and interactions with phosphoinositides and with SNX2.
13. Virbasius JV, Song X, Pomerleau DP, et al.: Activation of the Akt-related cytokine-independent survival kinase requires interaction of its phox domain with endosomal phosphatidylinositol 3-phosphate. Proc Natl Acad Sci U S A 2001, 98:12908-12913. In this study, it was demonstrated that the cytokine-independent survival kinase bound PI(3)P selectively through its PX domain, targeting this kinase to endosomal membranes, thereby activating it.
14. Song X, Xu W, Zhang A, et al.: Phox homology domains specifically bind phosphatidylinositol phosphates. Biochemistry 2001, 40:8940-8944.
15. phox PX domain.
16. phox.
17. phox was solved by NMR. An intramolecular interaction in this protein between the PXXP motif in the PX domain and an SH3 domain is described.
18. Heyworth PG, Cross AR: Chronic granulomatous disease mutations and the PX domain. Nat Cell Biol 2002, 4:E110.
19. Pawson T: Protein modules and signaling networks. Nature 1995, 373:573-580.
20. Thrasher AJ, Keep NH, Wientjes F, et al.: Chronic granulomatous disease. Biochim Biophys Acta 1994, 1227:1-24.
21. Wishart MJ, Taylor GS, and Dixon JE: Phoxy lipids: Revealing px domains as phosphoinositide binding modules. Cell 2001, 105:817-820.
22. Ellson CD, Anderson KE, Morgan G, et al.: Phosphatidylinositol 3-phosphate is generated in phagosomal membranes. Curr Biol 2001, 11:1631-1635. This article showed that the phagosomal membrane is transiently enriched in PI(3)P upon phagocytosis through fusion with endosomal vesicles and through synthesis.
23. Allen LA, DeLeo FR, Gallois A, et al.: Transient association of the nicotinamide adenine dinucleotide phosphate oxidase subunits p47phox and p67phox with phagosomes in neutrophils from patients with X-linked chronic granulomatous disease. Blood 1999, 93:3521-3530.
24. Wientjes FB, Reeves EP, Soskic V, et al.: The NADPH oxidase components p47phox and p40phox bind to moesin through their PX domain. Biochem Biophys Res Commun 2001, 289:382-388.
25. Hirao M, Sato N, Kondo T, et al.: Regulation mechanism of ERM ezrin/radixin/moesin) protein/plasma membrane association: Possible involvement of phosphatidylinositol turnover and Rho-dependent signaling pathway. J Cell Biol 1996, 135:37-51.
26. Haft CR, de la Luz S, Barr VA, et al.: Identification of a family of sorting nexin molecules and characterization of their association with receptors. Mol Cell Biol 1998, 18:7278-7287.
27. Teasdale RD, Loci D, Houghton F, et al.: A large family of endosome-localized proteins related to sorting nexin 1. Biochem J 2001, 358:7-16.
28. Phillips SA, Barr VA, Haft DH, et al.: Identification and characterization of SNX15, a novel sorting nexin involved in protein trafficking. J Biol Chem 2001, 276:5074-5084.
29. Xu Y, Hortsman H, Seet L, et al.: SNX3 regulates endosomal function through its PX-domain-mediated interaction with Ptdlns(3)P. Nat Cell Biol 2001, 3:658-666. Some properties of human SNX3 are described. It localized to early and recycling endosomes. For this, it required an intact PX domain, which was shown to bind PI(3)P.
30. Boeddinghaus C, Merz AJ, Laage R, et al.: A cycle of Vam7p release from and Ptdlns 3-P-dependent rebinding to the yeast vacuole is required for homotypic vacuole fusion. J Cell Biol 2002, 157:79-89.