Expression of TIMP-1 in Pichia pastoris. Selection of an anti-TIMP-1 specific single-chain Fv antibody from a large non-immune library

Clinica Chimica Acta

Volumn 327, Issue 1-2, 2003, Pages 171-179

  Wozniak, Gordana ^a,b   Obermayr, Eva ^a   Jeras, Matjaz ^b   Knezevic, Mio ^b,c   Rüker, Florian ^a  

^a UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

^b BLOOD TRANSFUSION CENTRE OF SLOVENIA   (Slovenia)

^c Transcell   (Slovenia)

Author keywords

Alkaline phosphatase; ELISA; Fusion proteins; Phage display; Single chain Fv fragments; Tissue inhibitor of metalloproteinase 1

Indexed keywords

ALKALINE PHOSPHATASE; ANTIGEN; ENZYME ANTIBODY; HYBRID PROTEIN; MONOCLONAL ANTIBODY; PEPTIDE LIBRARY; RECOMBINANT PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TISSUE INHIBITOR OF METALLOPROTEINASE 1; IMMUNOGLOBULIN FRAGMENT; IMMUNOGLOBULIN FV;

ANTIBODY ISOLATION; ANTIBODY SPECIFICITY; ANTIGEN PURIFICATION; ARTICLE; BACTERIOPHAGE; CONJUGATION; EXPRESSION VECTOR; GENETIC CODE; IMMUNOREACTIVITY; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROMOTER REGION; QUANTITATIVE ANALYSIS; BIOSYNTHESIS; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETICS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; PICHIA;

PICHIA PASTORIS;

ALKALINE PHOSPHATASE; ANTIBODIES, MONOCLONAL; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOGLOBULIN FRAGMENTS; PICHIA; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; TISSUE INHIBITOR OF METALLOPROTEINASE-1;

EID: 0037212887     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-8981(02)00372-8     Document Type: Article

References (33)

1. Armstrong D.G., Jude E.B. The role of matrix metalloproteinases in wound healing. J. Am. Podiatr. Med. Assoc. 92:2002;12-18.
2. Kikuchi K., Kubo M., Sato S., Fujimoto M., Tamaki K. Serum tissue inhibitor of metalloproteinases in patients with systemic sclerosis. J. Am. Acad. Dermatol. 33:1995;973-978.
3. Stetler-Stevenson W.G., Krutzsch H.C., Liotta L.A. Tissue inhibitor of metalloproteinase (TIMP-2). A new member of the metalloproteinase inhibitor family. J. Biol. Chem. 264:1989;17374-17378.
4. Howard E.W., Bullen E.C., Banda M.J. Preferential inhibition of 72- and 92-kDa gelatinases by tissue inhibitor of metalloproteinases-2. J. Biol. Chem. 266:1991;13070-13075.
5. Howard E.W., Bullen E.C., Banda M.J. Regulation of the autoactivation of human 72-kDa progelatinase by tissue inhibitor of metalloproteinases-2. J. Biol. Chem. 266:1991;13064-13069.
6. DeClerck Y.A., Yean T.D., Lu H.S., Ting J., Langley K.E. Inhibition of autoproteolytic activation of interstitial procollagenase by recombinant metalloproteinase inhibitor MI/TIMP-2. J. Biol. Chem. 266:1991;3893-3899.
7. Williamson R.A., Marston F.A., Angal S., Koklitis P., Panico M., Morris H.R.et al. Disulphide bond assignment in human tissue inhibitor of metalloproteinases (TIMP). Biochem. J. 268:1990;267-274.
8. Murphy G., Willenbrock F. Tissue inhibitors of matrix metalloendopeptidases. Methods Enzymol. 248:1995;496-510.
9. Bigg H.F., Shi Y.E., Liu Y.E., Steffensen B., Overall C.M. Specific, high affinity binding of tissue inhibitor of metalloproteinases-4 (TIMP-4) to the COOH-terminal hemopexin-like domain of human gelatinase A. TIMP-4 binds progelatinase A and the COOH-terminal domain in a similar manner to TIMP-2. J. Biol. Chem. 272:1997;15496-15500.
10. Khokha R., Zimmer M.J., Graham C.H., Lala P.K., Waterhouse P. Suppression of invasion by inducible expression of tissue inhibitor of metalloproteinase-1 (TIMP-1) in B16-F10 melanoma cells. J. Natl. Cancer Inst. 84:1992;1017-1022.
11. Khokha R., Zimmer M.J., Wilson S.M., Chambers A.F. Up-regulation of TIMP-1 expression in B16-F10 melanoma cells suppresses their metastatic ability in chick embryo. Clin. Exp. Metastasis. 10:1992;365-370.
12. MacDougall J.R., Bani M.R., Lin Y., Muschel R.J., Kerbel R.S. 'Proteolytic switching': opposite patterns of regulation of gelatinase B and its inhibitor TIMP-1 during human melanoma progression and consequences of gelatinase B overexpression. Br. J. Cancer. 80:1999;504-512.
13. Nuovo G.J., MacConnell P.B., Simsir A., Valea F., French D.L. Correlation of the in situ detection of polymerase chain reaction-amplified metalloproteinase complementary DNAs and their inhibitors with prognosis in cervical carcinoma. Cancer Res. 55:1995;267-275.
14. Baker T., Tickle S., Wasan H., Docherty A., Isenberg D., Waxman J. Serum metalloproteinases and their inhibitors: markers for malignant potential. Br. J. Cancer. 70:1994;506-512.
15. Zucker S., Lysik R.M., DiMassimo B.I., Zarrabi H.M., Moll U.M., Grimson R.et al. Plasma assay of gelatinase B: tissue inhibitor of metalloproteinase complexes in cancer. Cancer. 76:1995;700-708.
16. Gohji K., Fujimoto N., Fujii A., Komiyama T., Okawa J., Nakajima M. Prognostic significance of circulating matrix metalloproteinase-2 to tissue inhibitor of metalloproteinases-2 ratio in recurrence of urothelial cancer after complete resection. Cancer Res. 56:1996;3196-3198.
17. Holten-Andersen M.N., Murphy G., Nielsen H.J., Pedersen A.N., Christensen I.J., Hoyer-Hansen G.et al. Quantitation of TIMP-1 in plasma of healthy blood donors and patients with advanced cancer. Br. J. Cancer. 80:1999;495-503.
18. Kodama S., Iwata K., Iwata H., Yamashita K., Hayakawa T. Rapid one-step sandwich enzyme immunoassay for tissue inhibitor of metalloproteinases. An application for rheumatoid arthritis serum and plasma. J. Immunol. Methods. 127:1990;103-108.
19. Cooksley S., Hipkiss J.B., Tickle S.P., Holmes-Ievers E., Docherty A.J., Murphy G.et al. Immunoassays for the detection of human collagenase, stromelysin, tissue inhibitor of metalloproteinases (TIMP) and enzyme-inhibitor complexes. Matrix. 10:1990;285-291.
20. Clark I.M., Powell L.K., Wright J.K., Cawston T.E. Polyclonal and monoclonal antibodies against human tissue inhibitor of metalloproteinases (TIMP) and the design of an enzyme-linked immunosorbent assay to measure TIMP. Matrix. 11:1991;76-85.
21. Murphy G., Houbrechts A., Cockett M.I., Williamson R.A., O'Shea M., Docherty A.J. The N-terminal domain of tissue inhibitor of metalloproteinases retains metalloproteinase inhibitory activity. Biochemistry. 30:1991;8097-8102.
22. Plumpton T.A., Clark I.M., Plumpton C., Calvin J., Cawston T.E. Development of an enzyme-linked immunosorbent assay to measure total TIMP-1 (free TIMP-1 and TIMP-1 in combination with matrix-metalloproteinases) and measurement of TIMP 1 and CRP in serum. Clin. Chim. Acta. 240:1995;137-154.
23. Sheets M.D., Amersdorfer P., Finnern R., Sargent P., Lindquist E., Schier R.et al. Efficient construction of a large nonimmune phage antibody library: the production of high-affinity human single-chain antibodies to protein antigens. Proc. Natl. Acad. Sci. U. S. A. 95:1998;6157-6162.
24. Kerschbaumer R.J., Hirschl S., Schwager C., Ibl M., Himmler G. pDAP2: a vector for construction of alkaline phosphatase fusion-proteins. Immunotechnology. 2:1996;145-150.
25. DeClerck Y.A., Perez N., Shimada H., Boone T.C., Langley K.E., Taylor S.M. Inhibition of invasion and metastasis in cells transfected with an inhibitor of metalloproteinases. Cancer Res. 52:1992;701-708.
26. McCafferty J., Griffiths A.D., Winter G., Chiswell D.J. Phage antibodies: filamentous phage displaying antibody variable domains. Nature. 348:1990;552-554.
27. Jespers L.S., Roberts A., Mahler S.M., Winter G., Hoogenboom H.R. Guiding the selection of human antibodies from phage display repertoires to a single epitope of an antigen. Biotechnology (NY). 12:1994;899-903.
28. Kohl J., Rüker F., Himmler G., Razazzi E., Katinger H. Cloning and expression of an HIV-1 specific single-chain Fv region fused to Escherichia coli alkaline phosphatase. Ann. N.Y. Acad. Sci. 646:1991;106-114.
29. Harper K., Kerschbaumer R.J., Ziegler A., Macintosh S.M., Cowan G.H., Himmler G.et al. A scFv-alkaline phosphatase fusion protein which detects potato leafroll luteovirus in plant extracts by ELISA. J. Virol. Methods. 63:1997;237-242.
30. Skerra A., Plückthun A. Secretion and in vivo folding of the Fab fragment of the antibody McPC603 in Escherichia coli: influence of disulphides and cis-prolines. Protein Eng. 4:1991;971-979.
31. Kerschbaumer R.J., Hirschl S., Kaufmann A., Ibl M., Koenig R., Himmler G. Single-chain Fv fusion proteins suitable as coating and detecting reagents in a double antibody sandwich enzyme-linked immunosorbent assay. Anal. Biochem. 249:1997;219-227.
32. Gough K.C., Li Y., Vaughan T.J., Williams A.J., Cockburn W., Whitelam G.C. Selection of phage antibodies to surface epitopes of Phytophthora infestans. J. Immunol. Methods. 228:1999;97-108.
33. Kala M., Bajaj K., Sinha S. Direct antigen capture by soluble scFv antibodies. A method for detection, characterization, and determination of affinity. Appl. Biochem. Biotechnol. 90:2001;11-22.