Dinitrophenol derivatization of proteolytic products and its application in the assay of protease(s) activity

Journal of Neuroscience Methods

Volumn 120, Issue 2, 2002, Pages 155-161

  Bhaskar, Kiran ^a   Pavankumar Shetty, A ^a   Shareef, Momin M ^a   Ramamohan, Y ^a   Taranath Shetty, K ^a  

^a NATIONAL INSTITUTE OF MENTAL HEALTH AND NEUROSCIENCES   (India)

Author keywords

Calcium activated neutral protease; Dinitrofluorobenzene; Dinitrophenol derivative; DNFB; Proteases; Sanger's reagent

Indexed keywords

1 FLUORO 2,4 DINITROBENZENE; 2,4 DINITROPHENOL; AMINE; BRAIN ENZYME; CALPAIN; CASEIN; GLUTAMIC ACID; GLYCINE; PROTEINASE; TRICHLOROACETIC ACID; TRYPSIN;

ACIDITY; ALKALINITY; ANIMAL TISSUE; ARTICLE; BRAIN REGION; CONTROLLED STUDY; DERIVATIZATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SUBSTRATE; MOLECULAR STABILITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; QUANTITATIVE ANALYSIS; RAT; SPECTRAL SENSITIVITY; SPECTROPHOTOMETRY;

ANIMALS; BRAIN CHEMISTRY; CALCIUM; CASEINS; DINITROPHENOLS; ENDOPEPTIDASES; HYDROGEN-ION CONCENTRATION; INDICATORS AND REAGENTS; PROTEIN HYDROLYSATES; RATS; TRYPSIN;

EID: 0037201971     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(02)00196-6     Document Type: Article

References (24)

1. Andersson M., Sjostrand J., Petersen A., Karlsson J.O. Calcium-dependent proteolysis in rabbit lens epithelium after oxidative stress. Ophthalmic. Res. 30:1998;157-167.
2. Anson M.L. Carboxypeptidase I. The preparation of crystalline carboxypeptidase. J. Gen. Physiol. 20:1937;663-667.
3. Bi X., Chen J., Baudry M. Developmental changes in CANP activity, GluR1 receptors and in the effect of kainic acid treatment in rat brain. Neuroscience. 81:1997;1123-1135.
4. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
5. Choi D.W. Glutamate neurotoxicity and diseases of the nervous system. Neuron. 1:1989;623-634.
6. Jiang S.T., Wang J.H., Chang T., Chen C.S. A continuous method for measuring CANP activity. Anal. Biochem. 244:1997;233-238.
7. Jones L.T., Upson R.H., Haugland R.P., Voloshina N.P., Shou M., Haugland R.P. Quenched BODIPY dye-labeled casein substrates for the assay of protease activity by direct fluorescence measurement. Anal. Biochem. 251:1997;144-152.
8. Kachaturian Z.S. The role of calcium regulation in brain aging: reexamination of a hypothesis. Aging. 1:1989;17-34.
9. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
10. Mattson M.P., Cheng B., Davis D., Bryant K., Lieberburg I., Rydel R.E. Beta-Amyloid peptides destabilize calcium homeostasis and render human cortical neurons vulnerable to excitotoxicity. J. Neurosci. 12:1992;376-389.
11. + dependent neutral proteases from rabbit liver: purification and properties of the proenzymes. Arch. Biochem. Biophys. 232:1984;505-512.
12. Moor S., Stein W.H. Photometric ninhydrin method for use in the chromatography of amino acids. J. Biol. Chem. 176:1948;367-388.
13. Najm I., El-Skaf G., Tacco G., Vanderklish P., Lynch G., Baudry M. Seizure activity-induced changes in polyamine metabolism and neuronal pathology during the postnatal period in rat brain. Dev. Brain Res. 69:1992;11-21.
14. Nixon R.A. Calcium-activated neutral proteinases as regulators of cellular function. Implications for Alzheimer's disease pathogenesis. Ann. NY Acad. Sci. 568:1989;198-208.
15. Pope A., Nixon R.A. Proteases of human brain. Neurochem. Res. 7:1984;291-323.
16. Powers J.C., Kam C.M., Ricketts K.M., Casillas R.P. Cutaneous protease activity in the mouse ear vesicant model. J. Appl. Toxicol. 20:(S1):2000;S177-S182.
17. Saido T.C., Sorimachi H., Suzuki K. CANP: new perspectives in molecular diversity and physiological-pathological involvement. FASEB J. 8:1994;814-822.
18. Sanger F. The terminal peptides of Insulin. Biochem. J. 45:1949;563-573.
19. Simonson L., Baudry M., Siman R., Lynch G. Regional distribution of soluble CANP activated proteinase activity in neuronal and adult rat brain. Brain Res. 327:1985;153-159.
20. Tompa P., Schad E., Baki A., Alexa A., Batke J., Friedrich P. An ultrasensitive, continuous fluorometric assay for CANP activity. Anal. Biochem. 228:1995;287-293.
21. Van Salke D.D. A method for quantitative determination of aliphatic amino groups. J. Biol. Chem. 9:1911;185-204.
22. Van Salke D.D. The gasometric determination of aliphatic amino nitrogen in minute quantities. J. Biol. Chem. 16:1913;121-124.
23. Van Salke D.D. The quantitative determination of aliphatic amino groups II. J. Biol. Chem. 12:1912;275-284.
24. Waxman L. Calcium-activated proteases in mammalian tissues. Method Enzymol. 80:1981;664-680.