Thermal stability of Phaseolus vulgaris leucoagglutinin: A differential scanning calorimetry study

Journal of Biochemistry and Molecular Biology

Volumn 35, Issue 5, 2002, Pages 472-475

  Biswas, Shyamasri ^a   Kayastha, Arvind M ^a  

^a BANARAS HINDU UNIVERSITY   (India)

Author keywords

Differential scanning calorimetry; Phaseolus vulgaris leucoagglutinin; Plant lectins; Transition temperature

Indexed keywords

PHASEOLUS VULGARIS;

LEUKOAGGLUTININS, PLANTS; PHYTOHEMAGGLUTININ; PLANT LECTIN;

ARTICLE; BEAN; CHEMISTRY; DIFFERENTIAL SCANNING CALORIMETRY; PH; PROTEIN DENATURATION; TEMPERATURE;

CALORIMETRY, DIFFERENTIAL SCANNING; HYDROGEN-ION CONCENTRATION; PHASEOLUS; PHYTOHEMAGGLUTININS; PLANT LECTINS; PROTEIN DENATURATION; TEMPERATURE;

EID: 0037200981     PISSN: 12258687     EISSN: 12258687     Source Type: Journal    
DOI: 10.5483/bmbrep.2002.35.5.472     Document Type: Article

References (13)

1. Biswas, S. (2002) Biophysical studies on β-galactosidase and lymphocyte agglutinating lectin from kidney beans (Phaseolus vulgaris). Ph.D. Dissertation, Banaras Hindu University, Varanasi, pp. 1-138.
2. Chrispeels, M. J. and Raikhel, N. V. (1991) Lectins, lectin genes, and their roles in plant defense. Plant Cell 3, 1-9.
3. Edelhoch, H. (1967) Spectroscopic determination of tryptophan and tyrosine in proteins. Biochemistry 6, 1948-1954.
4. Hamelryck, T. W., Dao-Thi, M., Poortmans, F., Chrispeels, M. J., Wyns, L. and Loris, R. (1996) The crystallographic structure of phytohemagglutinin-L. J. Biol. Chem. 271, 20479-20485.
5. Loris, R., Hamelryck, T. W., Bouckaert, J. and Wyns, L. (1998) Legume lectin structure. Biochim. Biophys. Acta 1383, 9-36.
6. Manoj, N., Srinivas, V. R., Surolia, A., Vijayan, M. and Suguna, K. (2000) Carbohydrate specificity and salt bridge mediated conformational change in acidic winged bean agglutinin. J. Mol. Biol. 302, 1129-1137.
7. Pace, C. N., Vajdos, F., Fee, L., Grimsley, G. and Gray, T. (1995) How to measure and predict the molar absorption coefficient of a protein. Protein Sci. 4, 2411-2423.
8. Pusztai, A. (1993) Dietary lectins are metabolic signals for the gut and modulate immune and hormone functions. Eur. J. Clin. Nutr. 47, 691-699.
9. Reddy, G. B., Bharadwaj, S. and Surolia, A. (1999) Thermal stability and mode of oligomerization of the tetrameric peanut agglutinin: A differential scanning calorimetry study. Biochemistry 38, 4464-4470.
10. Sanchez-Ruiz, J. M. (1995) Differential scanning calorimetry of proteins. Subcell. Biochem. 24, 133-76.
11. Schwarz, F. P., Puri, K. D., Bhat, R. G. and Surolia, A. (1993) Thermodynamics of monosaccharides binding to Concavalin A, Pea (Pisum sativum) lectin and lentil (Lens culinaris) lectin. J. Biol. Chem. 268, 7668-7677.
12. Serafini-Cess, F. Montanaro, L. and Sperti, S. (1980) Effect of temperature on haemagglutinating activity and on the conformation of leucoagglutinin, a lectin from Phaseolus vulgaris (red kidney bean). FEBS Lett. 120, 115-118.
13. Sharon, N. and Lis, H. (1990) Legume lectins- a large family of homologous proteins. FASEB J. 4, 3198-3208.