Cloning and characterization of WMSUI, a Williopsis saturnus var. mrakii gene encoding a new yeast SUN protein involved in the cell wall structure

Yeast

Volumn 19, Issue 13, 2002, Pages 1127-1138

  Guyard, Cyril ^a   Cailliez, Jean Charles ^a,b   Tissier, Jean Pierre ^c   Dei Cas, Eduardo ^a,d   Mercenier, Annick ^a   Menozzi, Franco D ^a,e  

^a INSTITUT PASTEUR DE LILLE   (France)

^b UNIVERSITÉ CATHOLIQUE DE LILLE   (France)

^c CEMAGREF   (France)

^d UNIV LILLE   (France)

^e INSTITUT DE BIOLOGIE DE LILLE   (France)

Author keywords

Sun gene family; Williopsis saturnus var. mrakii MUCL 41968; Yeast cell wall

Indexed keywords

CELL MEMBRANE PROTEIN; FUNGAL PROTEIN; PROTEIN SUN; SERINE; SIGNAL PEPTIDE; THREONINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CELL METABOLISM; CELL STRUCTURE; CELL WALL; CONTROLLED STUDY; ESCHERICHIA COLI; FUNGAL STRAIN; FUNGUS; GENE EXPRESSION; GENE ISOLATION; GENE PROBE; GENETIC ANALYSIS; GENETIC CODE; MOLECULAR CLONING; MULTIGENE FAMILY; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN FUNCTION; SACCHAROMYCES CEREVISIAE; SCANNING ELECTRON MICROSCOPY; SOUTHERN BLOTTING; TOXICITY; WILLIOPSIS SATURNUS; CHEMISTRY; FUNGAL GENE; GENETICS; MOLECULAR GENETICS; PHENOTYPE; PICHIA;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; WILLIOPSIS; WILLIOPSIS SATURNUS; WILLIOPSIS SATURNUS VAR. MRAKII;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELL WALL; CLONING, MOLECULAR; ESCHERICHIA COLI; FUNGAL PROTEINS; GENES, FUNGAL; MOLECULAR SEQUENCE DATA; PHENOTYPE; PICHIA; SACCHAROMYCES CEREVISIAE;

EID: 0037200732     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.898     Document Type: Article

References (26)

1. Adams A, Gottschling D, Kaiser CTS. 1997. Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press: New York.
2. Cabib E, Rob DH, Schmidt M, Crotti LB, Varma A. 2001. The yeast cell wall and septum as paradigms of cell growth and morphogenesis. J Biol Chem 276: 19679-19682.
3. Camougrand NM, Mouassite M, Velours GM, Guérin MG. 2000. The 'SUN' family: UTH1, an ageing gene, is also involved in the regulation of mitochondria biogenesis in Saccharomyces cerevisiae. Arch Biochem Biophys 375: 154-160.
4. Cappellaro C, Mrsa V, Tanner W. 1998. New potential cell wall glucanases of Saccharomyces cerevisiae and their involvement in mating. J Bacteriol 180: 5030-5037.
5. Emanuelsson O, Nielsen H, Brunak S, von Heijne G. 2000. Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J Mol Biol 300: 1005-1016.
6. Germain D, Thomas DY, Boileau G. 1993. Processing of Kex2 pro-region at two interchangeable cleavage sites. FEBS Lett 323: 129-131.
7. Guyard C, Séguy N, Cailliez J, et al. 2002. Characterisation of a Williopsis saturnus var. mrakii high molecular weight secreted killer toxin with broad-spectrum antimicrobial activity. J Antimicrob Chemother 49: 961-971.
8. Hansen JE, Lund O, Rapacki K, Brunak S. 1997. O-GLYCBASE version 2.0: a revised database of O-glycosylated proteins. Nucleic Acids Res 25: 278-282.
9. Hoffman CS, Winston F. 1987. A 10-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene 57: 267-272.
10. Kapteyn JC, Van Den Ende H, Klis FM. 1999. The contribution of cell wall proteins to the organization of the yeast cell wall. Biochim Biophys Acta 1426: 373-383.
11. Klis FM. 1994. Review: cell wall assembly in yeast. Yeast 10:851-869.
12. Kyte J, Doolittle RF. 1982. A simple method for displaying the hydropathic character of a protein. J Mol Biol 157: 105-132.
13. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
14. Lipke PN, Ovalle R. 1998. Cell wall architecture in yeast: new structure and new challenges. J Bacteriol 180: 3735-3740.
15. Mouassite M, Camougrand N, Schwob E, Demaison G, Laclau M, Guerin M. 2000a. The 'SUN' family: yeast SUN4/SCW3 is involved in cell septation. Yeast 16: 905-919.
16. Mouassite M, Guerin MG, Camougrand NM. 2000b. The SUN family of Saccharomyces cerevisiae: the double knock-out of UTHI and SIM1 promotes defects in nucleus migration and increased drug sensitivity. FEMS Microbiol Lett 182: 137-141.
17. Mrsa V, Ecker M, Strahl-Bolsinger S, Nimtz M, Lehle L, Tanner W. 1999. Deletion of new covalently linked cell wall glycoproteins alters the electrophoretic mobility of phosphorylated wall components of Saccharomyces cerevisiae. J Bacteriol 181: 3076-3086.
18. Nielsen H, Engelbrecht J, Brunak S, von Heijne G. 1997. A neural network method for identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Int J Neural Syst 8: 581-599.
19. Omi K, Sonoda H, Nagata K, Sugita K. 1999. Cloning and characterization of psu1(+), a new essential fission yeast gene involved in cell wall synthesis. Biochem Biophys Res Commun 262: 368-374.
20. Sambrook J, Fritsh EF, Maniatis T. 1989. Molecular Cloning, 2nd edn. Cold Spring Harbor Laboratory Press: New York.
21. Shahinian S, Bussey H. 2000. β-1,6-Glucan synthesis in Saccharomyces cerevisiae. Mol Microbiol 35: 477-489.
22. Skory CD, Freer SN. 1995. Cloning and characterization of a gene encoding a cell-bound, extracellular β-glucosidase in the yeast Candida wickerhamii. Appl Environ Microbiol 61: 518-525.
23. Smits GJ, van Den Ende H, Klis FM. 2001. Differential regulation of cell wall biogenesis during growth and development in yeast. Microbiology 147: 781-794.
24. Towbin H, Staehelin T, Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354.
25. van der Vaart JM, Caro LH, Chapman JW, Klis FM, Verrips CT. 1995. Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae. J Bacteriol 177: 3104-3110.
26. West RW Jr, Yocum RR, Ptashne M. 1984. Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: location and function of the upstream activating sequence UASG. Mol Cell Biol 4: 2467-2478.