Transfer of anti-TFAR19 monoclonal antibody into HeLa cells by in situ electroporation can inhibit the apoptosis

Life Sciences

Volumn 71, Issue 15, 2002, Pages 1771-1778

  Rui, Min ^a   Chen, Yingyu ^a   Zhang, Yingmei ^a   Ma, Dalong ^a  

^a PEKING UNIVERSITY HEALTH SCIENCE CENTER   (China)

Author keywords

Antibody; Apoptosis; In situ electroporation; TFAR19

Indexed keywords

GENE PRODUCT; MONOCLONAL ANTIBODY;

APOPTOSIS; ARTICLE; CONTROLLED STUDY; ELECTROPORATION; FLOW CYTOMETRY; HELA CELL; HUMAN; HUMAN CELL; MAMMAL CELL; PASSIVE IMMUNIZATION; PROTEIN FUNCTION;

ANTIBODIES, BLOCKING; ANTIBODIES, MONOCLONAL; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; BLOTTING, WESTERN; ELECTROPORATION; FEMALE; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; HELA CELLS; HUMANS; IMMUNOGLOBULIN G; MICROSCOPY, FLUORESCENCE; NEOPLASM PROTEINS;

EUKARYOTA; MAMMALIA; PROKARYOTA;

EID: 0037199869     PISSN: 00243205     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0024-3205(02)01943-4     Document Type: Article

References (12)

1. Electroporaton: Parameters affecting transfer of DNA into mammalian cells
2. Electroporation enhances c-myc antisense oligodeoxynucleotide efficacy
3. Introduction of antibody into viable cells using electroporation
4. PDCD5, a novel apoptosis-related gene cloned from human leukemia cell line TF-1, could enhance apoptosis of some tumor cells induced by growth factor withdrawal
5. Nuclear translocation of PDCD5(TFAR19), an early signal for apoptosis
6. Preparation and identification of monoclonal antibodies against human apoptosis-related protein TFAR19
7. Transfer of monoclonal antibodies into mammalian cells by electroporation
8. Introduction of large molecules into viable fibroblasts by electroporation: Optimization of loading and identification of labeled cellular compartments
9. High efficiency gene transfection by in situ electroporation of cultured cells
10. Efficient transfer of antibodies into mammalian cells by electroporation