Cloning, expression, secondary structure characterization of HMG box 1 of hUBF from E. coli and its binding to DNA

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1598, Issue 1-2, 2002, Pages 147-155

  Yang, Wulin ^a   Zeng, Wanyong ^a   Zhou, Dan ^a   Shi, Yunyu ^a  

^a UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

Author keywords

Circular dichroism; Four way DNA junction; FTIR; Gel mobility shift assay; HMG box; hUBF; SPR

Indexed keywords

AMINO ACID; BACTERIAL PROTEIN; DNA DIRECTED RNA POLYMERASE; HISTIDINE DERIVATIVE; MAGNESIUM ION; NICKEL; RECOMBINANT PROTEIN; HIGH MOBILITY GROUP B1 PROTEIN; UNCLASSIFIED DRUG; UPSTREAM BINDING FACTOR;

ALPHA HELIX; ARTICLE; BACTERIAL GENE; CARBOXY TERMINAL SEQUENCE; CHELATION; CIRCULAR DICHROISM; CONTROLLED STUDY; DNA BINDING; DNA DENATURATION; GEL FILTRATION; GEL MOBILITY SHIFT ASSAY; GENE EXPRESSION; GENE STRUCTURE; MOLECULAR CLONING; NONHUMAN; PH; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; SURFACE PLASMON RESONANCE; DNA RECOMBINATION; ESCHERICHIA COLI; HIGH MOBILITY GROUP BOX DOMAIN; HUMAN; IN VIVO STUDY; PROTEIN DENATURATION; PROTEIN EXPRESSION; PURIFICATION;

ESCHERICHIA COLI;

EID: 0037194343     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/s0167-4838(02)00367-9     Document Type: Article

References (41)

1. Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins
2. The DNA binding site of HMG1 protein is composed of two similar segments (HMG boxes), both of which have counterparts in other eukaryotic regulatory proteins
3. A signature for the HMG-1 box DNA-binding proteins
4. The HMG-1 box protein family: Classification and functional relationships
5. Homology model building of the HMG-1 box structural domain
6. A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes
7. A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif
8. LEF-1, a gene encoding a lymphoid-specific protein with an HMG domain, regulates T-cell receptor alpha enhancer function
9. Identification and cloning of TCF-1, a T lymphocyte-specific transcription factor containing a sequence-specific HMG box
10. A human placental cDNA clone that encodes nonhiston chromosomal protein HMG-1
11. A new group of chromatin-associated proteins with a high content of acidic and basic amino acids
12. The RNA polymerase I transcription factor xUBF contains 5 tandemly repeated HMG homology boxes
13. Similarity of human mitochondrial transcription factor 1 to high mobility group proteins
14. HMG box proteins bind to four-way DNA junctions in their open conformation
15. Cloning and structural analysis of cDNA and the gene for mouse transcription factor UBF
16. RUBF, an RNA polymerase I transcription factor from rats, produces DNase I footprints identical to those produced by xUBF, its homolog from frogs
17. Identification of two forms of the RNA polymerase I transcription factor UBF
18. Assembly of alternative multiprotein complexes directs rRNA promoter selectivity
19. Enhancers for RNA polymerase I in mouse ribosomal DNA
20. Human autoantibody to RNA polymerase I transcription factor hUBF, molecular identity of nucleolus organizer region autoantigen NOR-90 and ribosomal RNA transcription upstream binding factor
21. Functional differences between the two splice variants of the nucleolar transcription factor UBF: The second HMG box determines specificity of DNA binding and transcriptional activity
22. Multiple domains of the RNA polymerase I activator hUBF interact with the TATA-binding protein complex hSL1 to mediate transcription
23. XUBF contains a novel dimerization domain essential for RNA polymerase I transcription
24. HMG box 4 is the principal determinant of species specificity in the RNA polymerase I transcription factor UBF
25. Short-range DNA looping by the Xenopus HMG-box transcription factor, xUBF
26. Structure basis for DNA bending by the architectural transcription factor LEF-1
27. Molecular basis of human 46X,Y sex reversal revealed from the three dimensional solution structure of the human SRY-DNA complex
28. Structure of the A-domain of HMG1 and its interaction with DNA as studied by heteronuclear three- and four-dimensional NMR spectroscopy
29. Solution structure of a DNA-binding domain from HMG1
30. Structure of the HMG box motif in the B-domain of HMG1
31. The solution structure and dynamics of the DNA-binding domain of HMG-D from Drosophila melanogaster
32. Solution structure of the sequence-specific HMG box of the lymphocyte transcriptional activator Sox-4
33. Solution structure of the HMG protein NHP6A and its interaction with DNA reveals the structural determinants for non-sequence-specific binding
34. Tricine-sodium dodecyl sulfate polyacrylamide gel eletrophoresis for separation of proteins in the range from 1 to 100 kDa
35. Examination of the secondary structure of proteins by deconvolved FTIR spetra
36. New insight into protein secondary structure from resolution-enhanced infrared spectra
37. DNA binding by single HMG box model proteins
38. The stereochemistry of a four-way DNA junction: A theoretical study
39. Structure-specific binding of the two tandem HMG boxes of HMG1 to four-way junction DNA is mediated by the A domain
40. Binding of human mitochondrial transcription factor A, an HMG box protein, to a four-way DNA junction
41. Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion