Monitoring Azospirillum-wheat interactions using the gfp and gusA genes constitutively expressed from a new broad-host range vector

Journal of Biotechnology

Volumn 97, Issue 3, 2002, Pages 243-252

  Ramos, Humberto J O ^a   Roncato Maccari, Lauren D B ^a   Souza, Emanuel M ^a   Soares Ramos, Juliana R L ^a   Hungria, Mariangela ^b   Pedrosa, Fábio O ^a  

^a FEDERAL UNIVERSITY OF PARANÁ   (Brazil)

^b EMBRAPA CERRADOS   (Brazil)

Author keywords

Azospirillum; Broad host range plasmids; Colonization; GFP; GUS; Wheat

Indexed keywords

CELLS; ESCHERICHIA COLI; FLUORESCENCE; MICROSCOPIC EXAMINATION; PLANTS (BOTANY); STRAIN; TISSUE;

INOCULATION;

GENES;

BETA GLUCURONIDASE; GREEN FLUORESCENT PROTEIN;

ARTICLE; AZOSPIRILLUM BRASILENSE; BACTERIAL COLONIZATION; BACTERIAL STRAIN; BACTERIUM DETECTION; CELL AGGREGATION; CONTROLLED STUDY; ENDOPHYTE; ESCHERICHIA COLI; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; INOCULATION; NONHUMAN; PLANT GROWTH; PLANT ROOT; PLASMID; PRIORITY JOURNAL; REPLICON; WHEAT; XYLEM;

ALUMINUM SILICATES; AZOSPIRILLUM BRASILENSE; BACTERIAL ADHESION; GENE EXPRESSION; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENETIC MARKERS; GLUCURONIDASE; GREEN FLUORESCENT PROTEINS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; PLANT ROOTS; PLASMIDS; SENSITIVITY AND SPECIFICITY; SYMBIOSIS; TRITICUM;

AZOSPIRILLUM; AZOSPIRILLUM BRASILENSE; BROAD HOST RANGE PLASMIDS; ESCHERICHIA COLI; TRITICUM AESTIVUM;

EID: 0037190111     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(02)00108-6     Document Type: Article

References (42)

1. Use of lacZ fusions to study the expression of nif genes of Azospirillum brasilense in associations with plants
2. In situ localization of Azospirillum brasilense in the rhizosphere of wheat with fluorescently labeled rRNA-targeted oligonucleotide probes and scanning confocal laser microscope
3. Innoculation of field-grown wheat (Triticum aestivum) with Azospirillum spp. in Brazil
4. Inoculants of plant-promoting bacteria for use in agriculture
5. Interactions of Azospirillum spp. in soils: A review
6. Survival of Azospirillum brasilense in the bulk soil and rhizosphere of 23 soil types
7. Effect of inoculation of Azospirillum spp. on nitrogen accumulation by field-grown wheat
8. Green fluorescent protein as a marker for gene expression
9. Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5 and Tn10-derived mini-transposon
10. Plasposons: Modular self-cloning minitransposon derivatives for rapid genetic analysis of gram-negative bacterial genomes
11. History and new perspectives of diazotrophs in association with nonleguminous plants
12. Endophytic occurence of diazotrophic bacteria in non-leguminous crops
13. Use of green fluorescent protein to detect expression of nif genes of Azoarcus sp BH72, a grass-associated diazotroph, on rice roots
14. Applications of green fluorescent protein as a molecular marker in environmental microorganisms
15. Use of green fluorescent protein to visualize the early events of symbiosis between Rhizobium meliloti and alfafa (Medicago sativa)
16. Genetics and molecular biology of Azospirillum
17. Root colonization and systemic spreading of Azoarcus sp. strain BH72 in grasses
18. Assaying chimeric genes in plants, GUS gene fusion system
19. A transposable partitioning locus used to stabilize plasmid-borne hydrogen oxidation and trifolitoxin production genes in a Sinorhizobium meliloti strain
20. Excretion of ammonium by Azospirillum brasilense mutants resistant to ethylenediamine
21. Construction of new β-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement
22. Determination of viable cell count: Bacterial growth curves
23. Green fluorescent protein-marked Pseudomonas fluorescens: Localization, viability, and activity in the natural barley rhizosphere
24. Agronomic applications of Azospirillum-an evaluation of 20 years worldwide field inoculation
25. The development of Azospirillum as a commercial inoculant for improving crop yields
26. Regulation of nitrogen fixation (nif) genes of Azospirillum brasilense by nifA and ntrC (glnG) type genes
27. Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria
28. PBBR1-GFP: A broad-host-range vector for prokaryotic promoter studies
29. Endophytic and surface colonization of wheat roots (Triticum aestivum) by different Azospirillum brasilense strains studied with strain-specific monoclonal antibodies
30. Immunulogical studies of the wheat-root-colonization by Azospirillum brasilense strains Sp7 and Sp245 using strain-specific monoclonal antibodies
31. A broad host range mobilization system for 'in vivo' genetic engineering transposon mutagenesis in gram negative bacteria
32. Azospirillum, a free-living nitrogen-fixing bacteria closely associated with grasses: Genetic, biochemical and ecological aspects
33. Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize rhizobia interacting with plants
34. Mobilization function of the pBHR1 plasmid, a derivative of broad-host-range plasmid pBBR1
35. Colonization pattener of biocontrol strains Pseudomonas chlororaphis MA32 on barley seeds visualized by using grreen fluorescent protein
36. Bacterial genetics by flow cytrometry: Rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction
37. Spatial-temporal colonization patterns of Azospirillum brasilense on the wheat root surface and expression of the bacterial nifH gene during association
38. β-Glucuronidase (GUS) transposons for ecological and genetic studies of rhizobia and other Gram-negative bacteria
39. Bi-funcional gfp-and gusA-containing mini-Tn5 transposon derivatives for combined gene expression and bacterial localization studies