In vitro formation of corticospinal synapses in an organotypic slice co-culture

Neuroscience

Volumn 109, Issue 2, 2002, Pages 359-370

  Takuma, H ^a   Sakurai, M ^a,b   Kanazawa, I ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b TEIKYO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Corticospinal tract; Motor cortex; Pathfinding; Rat; Spinal cord

Indexed keywords

1 AMINO 3 HYDROXY 5 METHYL 4 ISOXAZOLEPROPIONATE; 1,1' DIOCTADECYL 3,3,3',3' TETRAMETHYLINDOCARBOCYANINE; BIOCYTIN; GLUTAMATE RECEPTOR; HORSERADISH PEROXIDASE; PROPIONIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL LABELING; CELL POLARITY; CELLULAR DISTRIBUTION; CERVICAL SPINAL CORD; CHEMICAL LABELING; CONTROLLED STUDY; EXCITATORY POSTSYNAPTIC POTENTIAL; EXPLANT; GRAY MATTER; IN VITRO STUDY; IN VIVO STUDY; MEMBRANE; MOTONEURON; MOTOR CORTEX; NERVE CELL; NERVE PROJECTION; NEWBORN; NONHUMAN; POROSITY; POSTSYNAPTIC POTENTIAL; PRIORITY JOURNAL; PYRAMIDAL TRACT; RAT; SPINAL CORD; STELLATE CELL; SYNAPSE; TISSUE CULTURE; TISSUE SLICE;

ANIMALS; ANIMALS, NEWBORN; CELL DIFFERENTIATION; CELL SIZE; COCULTURE TECHNIQUES; ELECTRIC STIMULATION; EXCITATORY POSTSYNAPTIC POTENTIALS; GABA ANTAGONISTS; MAGNESIUM; MOTOR CORTEX; MOTOR NEURONS; NEURONS; ORGAN CULTURE TECHNIQUES; PYRAMIDAL TRACTS; RATS; REACTION TIME; RECEPTORS, AMPA; SPINAL CORD; SYNAPSES;

EID: 0037185571     PISSN: 03064522     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0306-4522(01)00472-9     Document Type: Article

References (62)

1. Termination areas of corticobulbar and corticospinal fibres in the rat
2. Intracellular recording and labeling with biocytin-filled electrodes
3. Effects of limited direct stimulation of the medullary pyramidal tract on spinal motoneurons in the rat
4. Fluctuation analysis of neutral amino acid responses in cultured mouse spinal neurones
5. Giant synaptic potentials in immature rat CA3 hippocampal neurones
6. Formation of target-specific neuronal projections in organotypic slice cultures from rat visual cortex
7. Selective cell culture of brain cells by serum-free, hormone-supplemented media: A comparative morphological study
8. Two membrane protein fractions from rat central myelin with inhibitory properties for neurite growth and fibroblast spreading
9. GABA: An excitatory transmitter in early postnatal life
10. Selective elimination of transient corticospinal projections in the rat cervical spinal cord gray matter
11. Growth and targeting of subplate axons and establishment of major cortical pathways
12. On the development of the pyramidal tract in the rat. I. The morphology of the growth zone
13. Ventral and dorsal horn acetylcholinesterase neurons are maintained in organotypic cultures of postnatal rat spinal cord explants
14. Influence of the cortico-spinal system on cervical and lumbar motoneurons in the rat
15. Cortico-spinal connections in the rat. I. Monosynaptic and polysynaptic responses of cervical motoneurons to epicortical stimulation
16. Mixed cultures of cerebellum and inferior olive: Generation of complex spikes in Purkinje cells
17. Functional innervation of cultured hippocampal neurones by cholinergic afferents from co-cultured septal explants
18. Organotypic slice cultures: A technique has come of age
19. Development of glycine- and GABA-gated currents in rat spinal motoneurons
20. Requirement for subplate neurons in the formation of thalamocortical connections
21. A study in developing visual systems with a new method of staining neurones and their processes in fixed tissue
22. On the development of the pyramidal tract in the rat. II. An anterograde tracer study of the outgrowth of the corticospinal fibers
23. Cell migrations to the isocortex in the rat
24. Peripheral pathways are pioneered by an array of central and peripheral neurones in grasshopper embryos
25. Climbing fibre induced depression of both mossy fibre responsiveness and glutamate sensitivity of cerebellar Purkinje cells
26. Cortico-spinal connections in the rat. II. Oligosynaptic and polysynaptic responses of lumbar motoneurons to epicortical stimulation
27. Excitatory postsynaptic potentials evoked by ventral root stimulation in neonate rat motoneurons in vitro
28. Corticospinal tract regrowth
29. Axon guidance of outgrowing corticospinal fibres in the rat
30. Transient projections from rat occipital cortex are able to respond to a spinal target derived diffusible factor in vitro
31. Tropism and corticospinal target selection in the rat
32. Embryogenesis of peripheral nerve pathways in grasshopper legs. III. Development without pioneer neurons
33. Transient pioneer neurons are essential for formation of an embryonic peripheral nerve
34. Topographic specificity of corticospinal connections formed in explant coculture
35. Entorhinal axons project to dentate gyrus in organotypic slice co-culture
36. Corticomotoneuronal connections in the rat: Evidence from double-labeling of motoneurons and corticospinal axon arborizations
37. Retrograde tracers for light and electron microscopy
38. Development of the geniculocortical pathway in rats
39. Subplate neurons pioneer the first axon pathway from the cerebral cortex
40. The origin of corticospinal projection neurons in rat
41. The role of chloride transport in postsynaptic inhibition of hippocampal neurons
42. Development of projection neuron types, axon pathways, and patterned connections of the mammalian cortex
43. Neural dynamics in cortex-striatum co-cultures - I. Anatomy and electrophysiology of neuronal cell types
44. Organotypic cortex-striatum-mesencephalon cultures: The nigrostriatal pathway
45. An improved silver stain for developing nervous tissue
46. Synaptic modification of parallel fibre-Purkinje cell transmission in in vitro guinea-pig cerebellar slices
47. Growth and target finding by axons of the corticospinal tract in prenatal and postnatal rats
48. Topographic sequence of outgrowth of corticospinal axons in the rat: A study using retrograde axonal labeling with Fast blue
49. Change in chemoattractant responsiveness of developing axons at an intermediate target
50. Anterograde tracing with PHA-L and biocytin at the electron microscopic level
51. An organotypic spinal cord-dorsal root ganglion-skeltal muscle coculture of embryonic rat. I. The morphological correlates of the spinal reflex arcs
52. The development of the corticospinal projection
53. An organotypic spinal cord-dorsal root ganglion-skeltal muscle coculture of embryonic rat. II. Functional evidence for the formation of spinal reflex arcs in vitro
54. Inhibitory miniature synaptic potentials in rat motoneurons
55. Anatomy, development and lesion-induced plasticity of rodent corticospinal tract
56. Ascending and descending pathways in the spinal cord
57. Laminar differences in development of afferent innervation to striate cortex neurones in kittens
58. En passant neurotrophic action of an intermediate axonal target in the developing mammalian CNS
59. Early development of glycine- and GABA-mediated synapses in rat spinal cord
60. Neural connections between the lateral geniculate nucleus and visual cortex in vitro
61. Repulsive and attractive mechanisms for the formation of corticofugal projections