Militarinone A, a neurotrophic pyridone alkaloid from paecilomyces militaris

Organic Letters

Volumn 4, Issue 2, 2002, Pages 197-199

  Schmidt, Karen ^a   Gunther, Wolfgang ^a   Stoyanova, Stefka ^a   Schubert, Barbara ^a   Li, Zengzhi ^b   Hamburger, Matthias ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^b ANHUI AGRICULTURAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALOID; MILITARINONE A; NERVE GROWTH FACTOR; PYRIDONE DERIVATIVE;

ANIMAL; ARTICLE; CELL STRAIN; CHEMICAL STRUCTURE; CHEMISTRY; DRUG EFFECT; ISOLATION AND PURIFICATION; NUCLEAR MAGNETIC RESONANCE; PAECILOMYCES; RAT;

ALKALOIDS; ANIMALS; MOLECULAR STRUCTURE; NERVE GROWTH FACTORS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PAECILOMYCES; PC12 CELLS; PYRIDONES; RATS;

EID: 0037165320     PISSN: 15237060     EISSN: None     Source Type: Journal    
DOI: 10.1021/ol016920j     Document Type: Article

References (17)

1. Dedicated to Prof. Dr. W. Fleischhacker on the occasion of his 70th birthday.
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16. 2). Cell differentiation and spike induction were visualized after 24 h by phase contrast microscopy and effects scored with "1", if appendices appeared as long as cell body, and "2" and "3" if they were two or three times longer. Nerve growth factor (NGF) at 25 ng/mL was used as a positive control. Approximately 80% of cell aggregates were evaluated as "3" when tested with 1 at 33 μM concentration. 70% were either "2" or "3" at 10 μM, and 30% were differentiated into a neuron-like shape with spikes as long as their cell body at 3.3 μM.
17. Cytotoxicity was determined after 24 h of exposure using an LDH kit (Roche Diagnostics), relative to the vehicle as a negative control and Triton X 100-Iysed cells as positive control.