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Volumn 4, Issue 6, 2002, Pages 957-960

Mutational analysis of the enterocin favorskii biosynthetic rearrangement

Author keywords

[No Author keywords available]

Indexed keywords

BRIDGED COMPOUND; ENTEROCIN;

EID: 0037149649     PISSN: 15237060     EISSN: None     Source Type: Journal    
DOI: 10.1021/ol0255155     Document Type: Article
Times cited : (55)

References (26)
  • 14
    • 0042042035 scopus 로고    scopus 로고
    • unpublished results
    • The genes encM and encK are present on different transcripts. Their respective downstream genes encN and encJ are also inactivated as a result of polar effects in each mutant. Inactivation of these starter unit biosynthesis genes resulted in a 0% and 25% reduction in 1 biosynthesis, respectively, suggesting that EncN is extraneous and that other cellular thiolases can complement the loss of the dedicated thiolase EncJ (Xiang, L.; Moore, B. S., unpublished results).
    • Xiang, L.1    Moore, B.S.2
  • 16
    • 0042042036 scopus 로고    scopus 로고
    • note
    • 10 The encK single-crossover mutant S. maritimus KK was selected after propagating transconjugants on SGGP plates at 37°C. and apramycin-resistant colonies were confirmed by Southern hybridization with biotinylated encK. Biotin-labeling and detection of chemiluminescent positives were performed with the DNADetector HPR Southern Blotting Kit (KPL. Inc.) according to the manufacturer. In a similar fashion, the encM disruption vector pBM1 was constructed from an 879 bp internal fragment of encM amplified with the primers 5′-CGGACACAGCATGGCCGGGC-3′ and 5′-GTCGTCGGGCACGCGCGCC-3′ and conjugated into "S. maritimus" to yield the encM single-crossover mutant "S. maritimus" KM.
  • 17
    • 0042542862 scopus 로고    scopus 로고
    • note
    • 2O to 0.085% TFA in MeCN over a period of 40 min.
  • 18
    • 0041540773 scopus 로고    scopus 로고
    • note
    • 18 (Waters, 20 mm × 250 mm, 10 μm) HPLC employing gradient elution (40% MeOH/0.15% TEA to 100% MeOH over 50 min) at flow rate of 9.5 mL/min. The resulting fractions were pooled on the basis of retention time in the UV chromatogram yielding wailupemycin E (4, 5.9 mg), wailupemycin D (3, 3.4 mg), wailupemycin F (5, 5.4 mg), and wailupemycin G (6, 9.4 mg), which eluted at 17, 22, 27, and 28.5 min, respectively.
  • 19
    • 0043043721 scopus 로고    scopus 로고
    • note
    • 6, Δ + 0.4 mmu).
  • 21
    • 0043043722 scopus 로고    scopus 로고
    • note
    • 6, Δ -0.1 mmu).
  • 22
    • 0042542825 scopus 로고    scopus 로고
    • note
    • 5, Δ + 0.6 mmu).
  • 24
    • 0042542833 scopus 로고
    • Harris, T. M.; Harris, C. M. Pure Appl. Chem. 1986, 58, 283-294. Shen, Y.; Yoon, P.; Yu, T.-W.; Floss, H. G.; Hopwood, D. A.; Moore, B. S. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 3622-3627.
    • (1986) Pure Appl. Chem. , vol.58 , pp. 283-294
    • Harris, T.M.1    Harris, C.M.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.