Regulated expression of proteins in yeast using the MAL61-62 promoter and a mating scheme to increase dynamic range

Gene

Volumn 285, Issue 1-2, 2002, Pages 49-57

  Finley Jr , Russell L ^a   Zhang, Huamei ^a   Zhong, Jinhui ^a   Stanyon, Clement A ^a  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Glucose repression; Interaction mating; Maltose; Transcription; Yeast two hybrid

Indexed keywords

FUNGAL PROTEIN; GLUCOSE; MALTOSE;

ARTICLE; CONTROLLED STUDY; DIPLOIDY; FUNGAL STRAIN; GENE EXPRESSION REGULATION; GENE REPRESSION; GENETIC TRANSCRIPTION; MATING; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN INTERACTION; TWO HYBRID SYSTEM; YEAST;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BASE SEQUENCE; CYCLIN A; CYCLIN E; DIPLOIDY; DROSOPHILA; GALACTOSE; GENE EXPRESSION REGULATION; GENES, FUNGAL; GENETIC VECTORS; GENOTYPE; GLUCOSE; HAPLOIDY; MALTOSE; MOLECULAR SEQUENCE DATA; MONOSACCHARIDE TRANSPORT PROTEINS; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SERINE ENDOPEPTIDASES; SYMPORTERS; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 0037138377     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(02)00420-1     Document Type: Article

References (36)

1. Regulated expression of endonuclease EcoRI in Saccharomyces cerevisiae: Nuclear entry and biological consequences
2. An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast
3. A yeast mating-selection scheme for detection of protein-protein interactions
4. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
5. Automation of yeast two-hybrid screening
6. Dominant effects of tubulin overexpression in Saccharomyces cerevisiae
7. The yeast two-hybrid system: Prospects for protein linkage maps
8. Regulation of maltase synthesis in Saccharomyces carlsbergensis
9. A novel genetic system to detect protein-protein interactions
10. Interaction mating reveals binary and ternary connections between Drosophila cell cycle regulators
11. Two-hybrid analysis of genetic regulatory networks
12. Isolation of Drosophila cyclin D, a protein expressed in the morphogenetic furrow before entry into S phase
13. The Drosophila fushi tarazu polypeptide is a DNA-binding transcriptional activator in yeast cells
14. Expression of alcohol-inducible rabbit liver cytochrome P-450 3a (P-450IIE1) in Saccharomyces cerevisiae with the copper-inducible CUP1 promoter
15. Interaction trap/two-hybrid system to identify interacting proteins
16. Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2
17. The complete set of predicted genes from Saccharomyces cerevisiae in a readily usable form
18. A comprehensive two-hybrid analysis to explore the yeast protein interactome
19. A model fungal gene regulatory mechanism: The GAL genes of Saccharomyces cerevisiae
20. Targeting cyclin-dependent kinases in Drosophila with peptide aptamers
21. Regulated expression of a human interferon gene in yeast: Control by phosphate concentration or temperature
22. The UAS(MAL) is a bidirectional promoter element required for the expression of both the MAL61 and MAL62 genes of the Saccharomyces MAL6 locus
23. Fusion of GAL4-VP16 to a steroid-binding domain provides a tool for gratuitous induction of galactose-responsive genes in yeast
24. Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae
25. Genome-wide analysis of vaccinia virus protein-protein interactions
26. Regulatable promoters of Saccharomyces cerevisiae: Comparison of transcriptional activity and their use for heterologous expression
27. Identification of the upstream activating sequence of MAL and the binding sites for the MAL63 activator of Saccharomyces cerevisiae
28. Yeast recombination: The association between double-strand gap repair and crossing-over
29. A novel, highly regulated, rapidly inducible system for the expression of chicken progesterone receptor, cPRA, in Saccharomyces cerevisiae
30. Use of conditional promoters for expression of heterologous proteins in Saccharomyces cerevisiae
31. The organization and transcription of the galactose gene cluster of Saccharomyces
32. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae
33. Mammalian Ras interacts directly with the serine/threonine kinase Raf
34. Protein interaction mapping in C. elegans using proteins involved in vulval development
35. Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: Location and function of the upstream activating sequence UASG
36. Use of lacZ fusions to delimit regulatory elements of the inducible GAL1-GAL10 promoter in Saccharomyces cerevisiae