X-ray structure of pyruvate formate-lyase in complex with pyruvate and CoA. How the enzyme uses the Cys-418 thiyl radical for pyruvate cleavage

Journal of Biological Chemistry

Volumn 277, Issue 42, 2002, Pages 40036-40042

  Becker, Andreas ^a   Kabsch, Wolfgang ^a  

^a MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; CRYSTAL STRUCTURE;

X-RAY STRUCTURE;

ENZYMES;

COENZYME A; PYRUVIC ACID DERIVATIVE;

AMINO ACID ANALYSIS; ARTICLE; BINDING SITE; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME ACTIVE SITE; ENZYME STRUCTURE; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN STRUCTURE;

ACETYLTRANSFERASES; BINDING SITES; CATALYTIC DOMAIN; COENZYME A; CRYSTALLOGRAPHY, X-RAY; CYSTEINE; DATABASES; ESCHERICHIA COLI; IONS; KINETICS; MODELS, CHEMICAL; MODELS, MOLECULAR; OXAMIC ACID; PROTEIN BINDING; PROTEIN CONFORMATION; PYRUVATES; PYRUVIC ACID; SUBSTRATE SPECIFICITY;

EID: 0037131403     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M205821200     Document Type: Article

References (30)

1. Reichard, P., and Ehrenberg, A. (1983) Science 221, 514-519
2. Knappe, J., Neugebauer, F. A., Blaschkowski, H. P., and Gänzler, M. (1984) Proc. Natl. Acad. Sci. U. S. A. 81, 1332-1335
3. Stubbe, J., and van der Donk, W. A. (1998) Chem. Rev. 98, 705-762
4. Eklund, H., and Fontecave, M. (1999) Structure (Lond.) 7, R257-R262
5. Knappe, J., and Wagner, A. F. V. (2001) Adv. Protein Chem. 58, 277-315
6. Wagner, A. F. V., Frey, M., Neugebauer, F. A., Schäfer, W., and Knappe, J. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 996-1000
7. Sun, X., Ollagnier, S., Schmidt, P. P., Atta, M., Mulliez, E., Lepape, L., Eliasson, R., Graslund, A., Fontecave, M., Reichard, P., and Sjoberg, B. M. (1996) J. Biol. Chem. 271, 6827-6831
8. Logan, D. T., Andersson, J., Sjöberg, B.-M., and Nordlund, P. (1999) Science 283, 1499-1504
9. Leppämen, V. M., Merckel, M. C., Ollis, D. L., Wong, K. K., Kozarich, J. W., and Goldman, A. (1999) Structure (Lond.) 7, 733-744
10. Becker, A., Fritz-Wolf, K., Kabsch, W., Knappe, J., Schultz, S., and Wagner, A. F. V. (1999) Nat. Struct. Biol. 6, 969-975
11. Reichard, P. (1997) Trends Biochem. Sci. 22, 81-85
12. Frey, M., Rothe, M., Wagner, A. F. V., and Knappe, J. (1994) J. Biol. Chem. 269, 12432-12437
13. Himo, F., and Eriksson, L. A. (1998) J. Am. Chem. Soc. 120, 11449-11455
14. Knappe, J., Elbert, S., Frey, M., and Wagner, A. F. V. (1993) Biochem. Soc. Trans. 21, 731-734
15. Engel, C., and Wierenga, R. (1996) Curr. Opin. Struct. Biol. 6, 790-797
16. Knappe, J., and Wagner, A. F. V. (1995) Methods Enzymol. 258, 343-362
17. Kabsch, W. (1993) J. Appl. Crystallogr. 26, 795-800
18. Brunger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J. S., Kuszewski, J., Nilges, M., Pannu, N. S., Read, R. J., Rice, L. M., Simonson, T., and Warren, G. L. (1998) Acta Crystallogr. Sect. D Biol. Crystallogr. 54, 905-921
19. Jones, T. A., Zou, J. Y., Cowan, S. W., and Kjeldgaard, M. (1991) Acta Crystallogr. Sect. A 47, 110-119
20. Laskowski, R. A., MacArthur, M. W., Moss, D. S., and Thornton, J. M. (1993) J. Appl. Crystallogr. 26, 283-291
21. Kraulis, P. J. (1991) J. Appl. Crystallogr. 24, 946-950
22. Esnouf, R. M. (1997) J. Mol. Graphics 15, 132-134
23. Merritt, E. A., and Bacon, D. J. (1997) Methods Enzymol. 277, 505-524
24. Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. (1990) J. Mol. Biol. 215, 403-410
25. Thompson, J. D., Higgins, D. G., and Gibson, T. J. (1994) Nucleic Acids Res. 22, 4673-4680
26. Kabsch, W. (1978) Acta Crystallogr. Sect. A 34, 827-828
27. van Aalte, D. M. F., DiRusso, C. C., and Knudsen, J. (2001) EMBO J. 20, 2041-2050
28. Becker, A. (1995) Towards the Three-dimensional Structure of Pyruvate Formate-Lyase. Crystallization of the Protein and Search for Heavy Atom Derivatives-Photoaffinity Labeling Using S-ethyl-(8-azido)CoA. Ph.D. thesis, University of Heidelberg, Heidelberg, Germany
29. Knappe, J., Blaschkowski, H. P., Gröbner, P., and Schmitt, T. (1974) Eur. J. Biochem. 50, 253-263
30. Diederichs, K., and Karplus, P. A. (1997) Nat. Struct. Biol. 4, 269-275