Changes in cortical and cerebellar bcl-2 mRNA levels in the developing hydrocephalic rat (LEW-HYR) as measured by a real time quantified RT-PCR

Neuroscience

Volumn 114, Issue 1, 2002, Pages 165-171

  Yamada, Y ^a   Watanabe, Y ^a   Zhang, J ^b   Haraoka, J ^a   Ito, H ^a  

^a TOKYO MEDICAL UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

A real time quantified reverse transcriptase polymerase chain reaction; bcl 2 mRNA; Cerebellum; Congenital hydrocephalic rat; Cortex; Development

Indexed keywords

CHLOROFORM; ISOSORBIDE DINITRATE; MESSENGER RNA; PROTEIN BCL 2;

AMINO ACID SEQUENCE; ANALYZER; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; BRAIN VENTRICLE DILATATION; CELL COUNT; CELL DEATH; CEREBELLUM; CONTROLLED STUDY; DISEASE COURSE; FEMALE; HYDROCEPHALUS; NERVE SPROUTING; NEUROPROTECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SEQUENCE; SEQUENCE ANALYSIS;

AGING; ANIMALS; ANIMALS, NEWBORN; APOPTOSIS; BASE SEQUENCE; CELL DIFFERENTIATION; CEREBELLUM; CEREBRAL CORTEX; DNA, COMPLEMENTARY; DOWN-REGULATION; FEMALE; HYDROCEPHALUS; MALE; MOLECULAR SEQUENCE DATA; NEURONS; PROTO-ONCOGENE PROTEINS C-BCL-2; RATS; RATS, MUTANT STRAINS; RNA, MESSENGER; UP-REGULATION;

EID: 0037130586     PISSN: 03064522     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0306-4522(02)00210-5     Document Type: Article

References (30)

1. Allsopp T.E., Wyatt S., Paterson H.F., Davies A.M. The proto-oncogene bcl-2 can selectively rescue neurotrophic factor-dependent neurons from apoptosis. Cell. 73:1993;295-307.
2. Bustin S.A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25:2000;169-193.
3. Calvo E.L., Boucher C., Coolombe Z., Morisset J. Pancreatic GAPDH gene expression during ontogeny and acute pancreatitis induced by caerulein. Biochem. Biophys. Res. Commun. 235:1997;636-640.
4. Cheung N.S., Beart P.M., Pascoe C.J., John C.A., Bernard O. Human bcl-2 protects against AMPA receptor-mediated apoptosis. J. Neurochem. 74:2000;1613-1620.
5. Chomczynski P. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques. 15:1993;532-537.
6. Edwards D.R., Denhardt D.T. A study of mitochondrial and nuclear transcription with cloned cDNA probes. Changes in the relative abundance of mitochondrial transcripts after stimulation of quiescent mouse fibroblasts. Exp. Cell Res. 157:1985;127-143.
7. Farlie P.G., Dringen R., Rees S.M., Kannourakis G., Bernard O. Bcl-2 transgene expression can protect neurons against developmental and induced cell death. Proc. Natl. Acad. Sci. USA. 92:1995;4397-4401.
8. Gelmini S., Orlando C., Sestini R., Vona G., Pinzani P., Ruocco L., Pazzagli M. Quantitative polymerase chain reaction-based homogeneous assay with fluorogenic probes to measure c-erbB-2 oncogene amplification. Clin. Chem. 43:1977;752-758.
9. Hamburger V., Oppenheim R.W. Naturally occurring neuronal death in vertebrates. Neurosci. Commun. 1:1982;39-55.
10. Higashi K., Asahisa H., Mifune H. Pathological studied on the brain of congenital hydrocephalic rats (Abstract in English). Nerv. Syst. Child. 12:1987;1-9.
11. Holland P.M., Abramson R.D., Watson R., Gelfand D.H. Detection of specific polymerase chain reaction product by utilizing the 5′→3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA. 88:1991;7276-7280.
12. Kalinina O., Lebedeva I., Brown J., Silver J. Nanoliter scale PCR with TaqMan detection. Nucleic Acids Res. 25:1997;1999-2004.
13. Keino H., Masaki S., Kawarada Y., Naruse I. Apoptotic degeneration in the arhinencephalic brain of the mouse mutant Pdn/Pdn. Brain Res. Dev. Brain Res. 78:1994;161-168.
14. Lawrence M.S., Ho D.Y., Sun G.H., Steinberg G.K., Sapolsky R.M. Overexpression of bcl-2 with herpes simplex virus vectors protects CNS neurons against neurological insults in vitro and in vivo. J. Neurosci. 16:1996;486-496.
15. Maciejewska B., Lipowska M., Kowianski P., Domaradzka-Pytel B., Morys J. Postnatal development of the rat striatum - a study using in situ DNA end labeling technique. Acta Neurobiol. Exp. (Warsz). 58:1998;23-28.
16. Martinou J.C., Dubois-Dauphin M., Staple J.K., Rodriguez I., Frankowski H., Missotten M., Albertini P., Talabot D., Catsicas S., Pietra C., Huarte J. Overexpression of bcl-2 in transgenic mice protects neurons from naturally occurring cell death and experimental ischemia. Neuron. 13:1994;1017-1030.
17. Merry D.E., Veis D.J., Hickey W.F., Korsmeyer S.J. Bcl-2 protein expression is widespread in the developing nervous system and retained in the adult PNS. Development. 120:1994;301-311.
18. Miller M.W., Al-Ghoul W.M. Numbers of neurons in the developing principal sensory nucleus of the trigeminal nerve: Enhanced survival of early-generated neurons over late-generated neurons. J. Comp. Neurol. 330:1993;491-501.
19. Miyan J.A., Khan M.I., Kawarada Y., Sugiyama T., Bannister C.M. Cell death in the brain of the HTx rat. Eur. J. Pediatr. Surg. Suppl. 1:1998;43-48.
20. Montpied P., Weller M., Paul S.M. N-methyl-D-aspartate receptor agonists decrease proto-oncogene bcl-2 mRNA expression in cultured rat cerebellar granule neurons. Biochem. Biophys. Res. Commun. 195:1993;623-629.
21. Oliveira J.G., Prados R.Z., Guedes A.C., Ferreira P.C., Kroon E.G. The housekeeping gene glyceraldehyde-3-phosphate dehydrogenase is inappropriate as internal control in comparative studies between skin tissue and cultured skin fibroblasts using Northern blot analysis. Arch. Derm. Res. 291:1999;659-661.
22. Oppenheim R.W. Cell death during development of the neurons system. Annu. Rev. Neurosci. 14:1991;453-501.
23. Sato T., Irie S., Krajewski S., Reed J.C. Cloning and sequencing of a cDNA encoding the rat bcl-2 protein (Apoptosis; suppressor of programmed cell death). Gene. 140:1994;291-292.
24. Sengoku Y., Ito H., Watanabe Y. Up-regulation of cortical glutamate receptor, i.e., NMDA receptor, in the development of congenital hydrocephalic rats (LEW-HYR). J. Brain Sci. 23:1997;271-282.
25. Simonati A., Tosati C., Rosso T., Piazzola E., Rizzuto N. Cell proliferation and death: Morphological evidence during corticogenesis in the developing human brain. Microsc. Res. Tech. 45:1999;341-352.
26. Tanabe H., Eguchi Y., Kamada S., Martinou J.C., Tsujimoto Y. Susceptibility of cerebellar granule neurons derived from Bcl-2-deficient and transgenic mice to cell death. Eur. J. Neurosci. 9:1997;848-856.
27. Veis D.J., Sorenson C.M., Shutter J.R., Korsmeyer S.J. Bcl-2-deficient mice demonstrate fulminant lymphoid apoptosis, polycystic kidneys, and hypopigmented hair. Cell. 75:1993;229-240.
28. Winer J., Jung C.K., Shackel I., Williams P.M. Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. Anal. Biochem. 270:1999;41-49.
29. Yamada Y., Ito H., Watanabe Y. Changes in brain glutamine synthetase activity in congenital hydrocephalic rats (LEW-HYR) after ventriculoperitoneal shunt. Neurol. Med. Chir. (Tokyo). 37:1997;663-667.
30. Yamada Y., Ito H., Watanabe Y. Reduced brain glutamine synthetase activity in a new congenital hydrocephalic rats model (LEW-HYR). J. Brain Sci. 23:1997;17-32.