The expression of the Helicobacter pylori genes ureA and nap is higher in vivo than in vitro as measured by quantitative competitive reverse transcriptase-PCR

FEMS Immunology and Medical Microbiology

Volumn 32, Issue 3, 2002, Pages 219-226

  Blom, Kristina ^a   Svennerholm, Ann Mari ^a   Bölin, Ingrid ^a  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

Helicobacter pylori; In vivo; Neutrophil activating protein; Quantitative competitive reverse transcriptase PCR; Transcription; Urease

Indexed keywords

BACTERIAL PROTEIN; INTERLEUKIN 8; RIBOSOME RNA; RNA; UREASE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL GENETICS; BACTERIAL GROWTH; BACTERIAL INFECTION; BACTERIAL VIRULENCE; BRUCELLA; CONTROLLED STUDY; FEMALE; GENE EXPRESSION; GENETIC CODE; GENETIC TRANSCRIPTION; HELICOBACTER PYLORI; IN VITRO STUDY; IN VIVO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STOMACH;

ANIMALS; BACTERIAL PROTEINS; FEMALE; GENE EXPRESSION; HELICOBACTER PYLORI; MICE; MICE, INBRED C57BL; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; UREASE;

EID: 0037128134     PISSN: 09288244     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0928-8244(01)00300-5     Document Type: Article

References (26)

1. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori
2. Natural pathogens of laboratory mice, rats, and rabbits and their effects on research
3. The coccoid forms of Helicobacter pylori. Criteria for their viability
4. The accuracy of competitive RT-PCR using the right exogenous standard
5. Detection of spiral and coccoid forms of Helicobacter pylori using a murine monoclonal antibody
6. Helicobacter pylori virulence and genetic geography
7. Helicobacter pylori physiology predicted from genomic comparison of two strains
8. Effect of gastric pH on urease-dependent colonization of gnotobiotic piglets by Helicobacter pylori
9. Reverse transcription and polymerase chain reaction amplification of rRNA for detection of Helicobacter species
10. Characterization of a Helicobacter pylori neutrophil-activating protein
11. Quantitative RT-PCR: Pitfalls and potential
12. DNA probes to ribosomal RNA
13. Helicobacter pylori: The missing link in perspective
14. Nested-polymerase chain reaction for the detection of Helicobacter pylori infection with novel primers designed by sequence analysis of urease A gene in clinically isolated bacterial strains
15. Quantification of gene expression by competitive RT-PCR: The hCGβ/LHβ gene cluster
16. A standardized mouse model of Helicobacter pylori infection: Introducing the Sydney strain
17. flaA mRNA transcription level correlates with Helicobacter pylori colonisation efficiency in gnotobiotic piglets
18. Specific antibodies in sera and gastric aspirates of symptomatic and asymptomatic Helicobacter pylori-infected subjects
19. Neutrophil-activating protein mediates adhesion of Helicobacter pylori to sulfated carbohydrates on high-molecular-weight salivary mucin
20. Quantitative RNA Analysis. How to use NPAs, RT-PCR and Northerns for relative and absolute quantitation of mRNA
21. The neutrophil-activating protein (HP-NAP) of Helicobacter pylori is a protective antigen and a major virulence factor
22. Helicobacter pylori: A eubacterium lacking the stringent response
23. Carbohydrate binding specificity of the neutrophil-activating protein of Helicobacter pylori
24. The complete genome sequence of the gastric pathogen Helicobacter pylori
25. The Helicobacter pylori neutrophil-activating protein is an iron-binding protein with dodecameric structure
26. A urease-negative mutant of Helicobacter pylori constructed by allelic exchange mutagenesis lacks the ability to colonize the nude mouse stomach