Establishment of uncharacterized plasmids in Escherichia coli by in vitro transposition

FEMS Microbiology Letters

Volumn 217, Issue 2, 2002, Pages 249-254

  Agron, Peter G ^a   Sobecky, Patricia ^b   Andersen, Gary L ^a  

^a LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

^b GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

In vitro transposition; PGT5; Plasmid isolation; Plasmid recovery; Plasmid rescue; Pyrococcus abyssi

Indexed keywords

ARTICLE; DNA TRANSPOSITION; ESCHERICHIA COLI; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROKARYOTE; REPLICON;

BASE SEQUENCE; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; GENETIC VECTORS; MODELS, GENETIC; PLASMIDS; TRANSFORMATION, BACTERIAL;

ARCHAEA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA; PYROCOCCUS; PYROCOCCUS ABYSSI;

EID: 0037126651     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(02)01090-X     Document Type: Article

References (16)

1. Davison J. Genetic exchange between bacteria in the environment. Plasmid. 42:1999;73-91.
2. Reznikoff W.S., Bhasin A., Davies D.R., Goryshin I.Y., Mahnke L.A., Naumann T., Rayment I., Steiniger-White M., Twining S.S. Tn5: A molecular window on transposition. Biochem. Biophys. Res. Commun. 266:1999;729-734.
3. Goryshin I.Y., Reznikoff W.S. Tn5 in vitro transposition. J. Biol. Chem. 273:1998;7367-7374.
4. Zhou M., Bhasin A., Reznikoff W.S. Molecular genetic analysis of transposase-end DNA sequence recognition: cooperativity of three adjacent base-pairs in specific interaction with a mutant Tn5 transposase. J. Mol. Biol. 276:1998;913-925.
5. Roberts R.C., Burioni R., Helinski D.R. Genetic characterization of the stabilizing functions of a region of broad-host-range plasmid RK2. J. Bacteriol. 172:1990;6204-6216.
6. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 19:1982;259-268.
7. Alexeyev M.F. The pKNOCK series of broad host-range mobilizable suicide vectors for gene knockout and targeted DNA insertion into the chromosome of gram-negative bacteria. BioTechniques. 26:1999;824-828.
8. Clewell D.B., Helinski D.R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an open circular DNA form. Proc. Natl. Acad. Sci. USA. 62:1969;1159-1166.
9. Birnboim H.C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1979;1513-1523.
10. Gordon D., Abajian C., Green P. Consed: a graphical tool for sequence finishing. Genome Res. 8:1998;195-202.
11. Erauso G., Marsin S., Benbouzid-Rollet N., Baucher M.-F., Barbeyron T., Zivanovic Y., Prieur D., Forterre P. Sequence of plasmid pGT5 from the archaeon Pyrococcus abyssi: evidence for rolling-circle replication in a hyperthermophile. J. Bacteriol. 178:1996;3232-3237.
12. Skowronski E.W., Armstrong N., Andersen G., Macht M., McCready P.M. Magnetic, microplate-format plasmid isolation protocol for high-yield, sequencing-grade DNA. BioTechniques. 29:2000;786-792.
13. Calos M.P. DNA sequence for a low-level promoter of the lac repressor gene and an 'up' promoter mutation. Nature. 274:1978;762-765.
14. Womble D.D., Dong X., Wu R.P., Luckow V.A., Martinez A.F., Rownd R.H. IncFII plasmid incompatibility product and its target are both RNA transcripts. J. Bacteriol. 160:1984;28-35.
15. Ditta G., Stanfield S., Corbin D., Helinski D.R. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. USA. 77:1980;7347-7351.
16. Simon R., Priefer U., Puhler A. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Biotechnology. 1:1983;784-791.