Transcriptional and mutational analysis of the Helicobacter pylori urease promoter

FEMS Microbiology Letters

Volumn 213, Issue 1, 2002, Pages 27-32

  Davies, Beverly J ^a   De Vries, Nicolette ^b,c   Rijpkema, Sjoerd G ^d   Van Vliet, Arnoud H M ^b   Penn, Charles W ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b ERASMUS MEDICAL CENTER   (Netherlands)

^c VU UNIVERSITY MEDICAL CENTER   (Netherlands)

^d NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL   (United Kingdom)

Author keywords

Helicobacter; Mutational analysis; Palindrome; Reporter gene; Urease promoter

Indexed keywords

UREASE; NICKEL;

ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; ESCHERICHIA COLI; GENE DELETION; GENE EXPRESSION; GENE MUTATION; GENETIC TRANSCRIPTION; GENOTYPE; HELICOBACTER PYLORI; NONHUMAN; OPERON; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; DNA SEQUENCE; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; LACTOSE OPERON; MUTATION; OPERATOR GENE; PROTEIN MOTIF;

AMINO ACID MOTIFS; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HELICOBACTER PYLORI; LAC OPERON; MUTATION; NICKEL; OPERATOR REGIONS (GENETICS); PROMOTER REGIONS (GENETICS); SEQUENCE ANALYSIS, DNA; SUPPORT, NON-U.S. GOV'T; TRANSCRIPTION, GENETIC; UREASE;

HELICOBACTER; HELICOBACTER PYLORI;

EID: 0037118618     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(02)00759-0     Document Type: Article

References (24)

1. Helicobacter pylori
2. Urease
3. A urease-negative mutant of Helicobacter pylori constructed by allelic exchange mutagenesis lacks the ability to colonize the nude mouse stomach
4. Shuttle cloning and nucleotide sequences of Helicobacter pylori genes responsible for urease activity
5. +-gated urea channel: The link between Helicobacter pylori urease and gastric colonization
6. Synthesis and activity of Helicobacter pylori urease and catalase at low pH
7. Helicobacter pylori requires an acidic environment to survive in the presence of urea
8. Identification of the urease operon in Helicobacter pylori and its control by mRNA decay in response to pH
9. Nickel-responsive induction of urease expression in Helicobacter pylori is mediated at the transcriptional level
10. NikR mediates nickel-responsive transcriptional induction of urease expression in Helicobacter pylori
11. 80 family of promoter signals
12. Mutational analysis of the vacA promoter provides insight into gene transcription in Helicobacter pylori
13. Motility of Helicobacter pylori is coordinately regulated by the transcriptional activator FlgR, an NtrC homolog
14. Activation of Helicobacter pylori ureA promoter by a hybrid Escherichia coli-H. pylori rpoD gene in E. coli
15. A flagellar sheath protein of Helicobacter pylori is identical to HpaA, a putative N-acetylneuraminyllactose-binding hemagglutinin, but is not an adhesin for AGS cells
16. A PCR-based strategy for the rapid construction of defined bacterial deletion mutants
17. Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers
18. Identification of environmental stress-regulated genes in Helicobacter pylori by a lacZ reporter gene fusion system
19. Identification of virulence genes of Helicobacter pylori by random insertion mutagenesis
20. The -16 region, a vital sequence for the utilization of a promoter in Bacillus subtilis and Escherichia coli
21. DNA sequence elements located immediately upstream of the -10 hexamer in Escherichia coli promoters: A systematic study
22. Transcription and translation
23. Metronidazole resistance in Helicobacter pylori is due to null mutations in a gene (rdxA) that encodes an oxygen-insensitive NADPH nitroreductase