Proteolysis during the isoelectric focusing step of two-dimensional gel electrophoresis may be a common problem

Analytical Biochemistry

Volumn 311, Issue 2, 2002, Pages 182-186

  Finnie, Christine ^a   Svensson, Birte ^a  

^a CARLSBERG LABORATORY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

ISOELECTRIC FOCUSING; TWO-DIMENSIONAL GEL ELECTROPHORESIS;

ELECTROPHORESIS;

PROTEOME; VEGETABLE PROTEIN; BETA AMYLASE; PEPTIDE FRAGMENT; PROTEIN; PROTEINASE; PROTEINASE INHIBITOR;

ANALYTIC METHOD; ARTICLE; BARLEY; IMMUNOBLOTTING; ISOELECTRIC FOCUSING; NONHUMAN; PLANT SEED; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; TWO DIMENSIONAL GEL ELECTROPHORESIS; ENZYMOLOGY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PROTEOMICS; STANDARD;

HORDEUM VULGARE SUBSP. VULGARE;

BETA-AMYLASE; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENDOPEPTIDASES; HORDEUM; ISOELECTRIC FOCUSING; PEPTIDE FRAGMENTS; PROTEASE INHIBITORS; PROTEINS; PROTEOMICS;

EID: 0037114993     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00389-5     Document Type: Article

References (6)

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2. J.C. Zadoks, T.T. Chang, C.F. Konzak, A decimal code for the growth stages of cereals, Weed Res. 14 (1974) 415-421.
3. A. Shevchenko, M. Wilm, O. Vorm, M. Mann, Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels, Anal. Chem. 68 (1996) 850-858.
4. A.W. MacGregor, D.E. LaBerge, O.S. Meredith, Changes in barley kernels during growth and maturation, Cereal Chem. 48 (1971) 255-269.
5. C. Colas des Francs, H. Thiellement, D. de Vienne, Analysis of leaf proteins by two-dimensional gel electrophoresis, Plant Physiol. 78 (1985) 178-182.
6. W.J. Hurkman, C.K. Tanaka, Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis, Plant Physiol. 81 (1986) 802-806.