A simple method for the determination of dissociation constants by displacement ELISA

Journal of Immunological Methods

Volumn 270, Issue 2, 2002, Pages 155-162

  Orosz, Ferenc ^a   Ovádi, Judit ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

Displacement; Dissociation constant; ELISA; Hexokinase; Linearization; Tubulin

Indexed keywords

ANTIGEN; ENZYME ANTIBODY; HEXOKINASE; TUBULIN;

ANTIBODY SPECIFICITY; ANTIGEN ANTIBODY COMPLEX; ARTICLE; BLOOD LEVEL; DILUTION; DISSOCIATION CONSTANT; ENZYME LINKED IMMUNOSORBENT ASSAY; LINEAR SYSTEM; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; PROTEIN INTERACTION; QUALITATIVE ANALYSIS; STOICHIOMETRY;

ANTIBODIES; ANTIGENS; ENZYME-LINKED IMMUNOSORBENT ASSAY;

EID: 0037114277     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00295-8     Document Type: Article

References (23)

1. Aleshin A.E., Zeng C., Bourenkov G.P., Bartunik H.D., Fromm H.J., Honzatko R.B. The mechanism of regulation of hexokinase: new insights from the crystal structure of recombinant human brain hexokinase complexed with glucose and glucose-6-phosphate. Structure. 6:1998;39.
2. Butler J.E. The Amplified ELISA: Principles of and Applications for the Comparative Quantitation of Class and Subclass Antibodies and the Distribution of Antibodies and Antigens in Biochemical Separates. 1981;Academic Press, Orlando.
3. Engwall E. Enzyme immunoassay ELISA and EMIT. Methods Enzymol. 70:1980;419.
4. Fuchs H., Gessner R. The result of equilibrium-constant calculations strongly depends on the evaluation method used on the type of experimental errors. Biochem. J. 359:2001;411.
5. Fuchs H., Orberger G., Tauber R., Gessner R. Direct calibration ELISA: a rapid method for the simplified determination of association constants of unlabelled biological molecules. J. Immunol. Methods. 188:1995;197.
6. Hoylaerts M.F., Bollen A., De Broe M.E. The application of enzyme kinetics to the determination of dissociation constants for antigen-antibody interactions in solution. J. Immunol. Methods. 126:1990;253.
7. Jana C.K., Ali E. High resolution affinity chromatography of an anti-steroid antiserum by gradient evolution with propionic acid. J. Immunol. Methods. 225:1999;95.
8. Lehotzky A., Telegdi M., Liliom K., Ovádi J. Interaction of phosphofructokinase with tubulin and microtubule: quantitative evaluation of the mutual effects. J. Biol. Chem. 268:1993;10888.
9. Liliom K., Orosz F., Horváth L., Ovádi J. Quantitative evaluation of indirect ELISA. Effect of calmodulin antagonists on antibody binding to calmodulin. J. Immunol. Methods. 143:1991;119.
10. Liliom K., Lehotzky A., Molnár A., Ovádi J. Characterization of tubulin-drug interactions by ELISA. Anal. Biochem. 228:1995;18.
11. Lineweaver H., Burk D. The determination of enzyme dissociation constants. J. Am. Chem. Soc. 56:1934;658.
12. Marmillot P., Keith T., Srivastava D.K., Knull H.R. Effect of tubulin on the activity of the muscle isoenzyme of lactate dehydrogenase. Arch. Biochem. Biophys. 315:1994;467.
13. Muronetz V.I., Wang Z.-X., Keith T.J., Knull H.R., Srivastava D.K. Binding constants and stoichiometrics of GAPD-tubulin complexes. Arch. Biochem. Biophys. 313:1994;253.
14. Na C.N., Timasheff S.N. Interaction of vinblastine with calf brain tubulin: multiple equilibria. Biochemistry. 25:1986;6214.
15. Nygren H., Stenberg M. Calibration by ellipsometry of the enzyme-linked immunosorbent assay. J. Immunol. Methods. 80:1985;15.
16. Nygren H., Stenberg M. Kinetics of antibody-binding to surface-immobilized antigen: influence of mass transport on the enzyme-linked immunosorbent assay (ELISA). J. Colloid Interface Sci. 107:1985;560.
17. Nygren H., Stenberg M. Immunochemistry at interfaces. Immunology. 66:1989;321.
18. Nygren H., Kaartinen M., Stenberg M. Determination by ellipsometry of the affinity of monoclonal antibodies. J. Immunol. Methods. 92:1986;219.
19. Scatchard G. The attraction of proteins for small molecules and ions. Ann. N. Y. Acad. Sci. 51:1949;660.
20. Vértessy G.B., Orosz F., Kovács J., Ovádi J. Alternative binding of two sequential glycolytic enzymes to microtubules: molecular studies in phosphofructokinase/aldolase/microtubule system. J. Biol. Chem. 272:1997;25542.
21. Wágner G., Kovács J., Lôw P., Orosz F., Ovádi J. Tubulin and microtubule are potential targets for brain hexokinase binding. FEBS Lett. 509:2001;81.
22. Werthén M., Nygren H. Effect of antibody affinity on the isotherm of antibody binding to surface-immobilized antigen. J. Immunol. Methods. 115:1988;71.
23. Wilson J.E. Hexokinases. Rev. Physiol., Biochem. Pharmacol. 126:1995;65.