The use of real-time PCR with fluorogenic probes for the rapid selection of mutant neuroectodermal grafts

Journal of Neuroscience Methods

Volumn 120, Issue 1, 2002, Pages 85-94

  Zumsteg, Valérie ^a   Boison, Detlev ^a,b  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b ETH ZURICH   (Switzerland)

Author keywords

Adenosine; Cell therapy; Epilepsy; Grafting; Neuroectoderm; Neuronal stem cells; Taqman technology

Indexed keywords

ADENOSINE; ADENOSINE KINASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL SURVIVAL; CELL TRANSPLANTATION; CELL VIABILITY; CONTROLLED STUDY; EMBRYO; ENZYME ASSAY; EPILEPSY; GENE DISRUPTION; GENOTYPE; LATERAL BRAIN VENTRICLE; MOUSE; NEUROECTODERM; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ADENOSINE KINASE; ANIMALS; BRAIN TISSUE TRANSPLANTATION; CELLS, CULTURED; ECTODERM; EMBRYO; FLUORESCENT DYES; GENOTYPE; LATERAL VENTRICLES; MICE; MICE, MUTANT STRAINS; MICE, TRANSGENIC; NEURONS; POLYMERASE CHAIN REACTION; TRANSPLANTS;

EID: 0037109262     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(02)00186-3     Document Type: Article

References (24)

1. Boison D., Scheurer L., Tseng J.L., Aebischer P., Mohler H. Seizure suppression in kindled rats by intraventricular grafting of an adenosine releasing synthetic polymer. Exp. Neurol. 160:1999;164-174.
2. Boison D., Scheurer L., Zumsteg V., Rülicke T., Litynski P., Fowler B., Brandner S., Mohler H. Neonatal hepatic steatosis by disruption of the adenosine kinase gene. Proc. Natl. Acad. Sci. USA. 99:2002;6985-6990.
3. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
4. Brundin P., Schierle G.S.K. Neuroprotective strategies in neuronal grafting. Dunnett S.B., Boulton A.A., Baker G.B., Neuronal Transplantation Methods. 2000;411-441 Humana Press, Totowa, NJ.
5. Debus E., Weber K., Osborn M. Monoclonal antibodies specific for glial fibrillary acidic (GFA) protein and for each of the neurofilament triplet polypeptides. Differentiation. 25:1983;193-203.
6. Delaney S.M., Geiger J.D. Brain regional levels of adenosine and adenosine nucleotides in rats killed by high-energy focused microwave irradiation. J. Neurosci. Methods. 64:1996;151-156.
7. Devinsky O. Patients with refractory seizures. N. Engl. J. Med. 340:1999;1565-1570.
8. Dunnett S.B., Bjorklund A., Lindvall O. Cell therapy in Parkinson's disease - stop or go? Nat. Rev. Neurosci. 2:2001;365-369.
9. Dunwiddie T.V. Adenosine and suppression of seizures. Adv. Neurol. 79:1999;1001-1010.
10. Dunwiddie T.V., Masino S.A. The role and regulation of adenosine in the central nervous system. Ann. Rev. Neurosci. 24:2001;31-55.
11. Eriksson P.S., Perfilieva E., Bjork-Eriksson T., Alborn A.M., Nordborg C., Peterson D.A., Gage F.H. Neurogenesis in the adult human hippocampus. Nat. Med. 4:1998;1313-1317.
12. Fredholm B.B. Adenosine and neuroprotection. Int. Rev. Neurobiol. 40:1997;259-280.
13. Henneberger C., Grantyn R., Rothe T. Rapid genotyping of newborn gene mutant mice. J. Neurosci. Methods. 100:2000;123-126.
14. Huber A., Padrun V., Déglon N., Aebischer P., Möhler H., Boison D. Grafts of adenosine releasing cells suppress seizures in kindling epilepsy. Proc. Natl. Acad. Sci. USA. 98:2001;7611-7616.
15. Isenmann S., Brandner S., Aguzzi A. Neuroectodermal grafting: a new tool for the study of neurodegenerative diseases. Histol. Histopathol. 11:1996;1063-1073.
16. Isenmann S., Brandner S., Sure U., Aguzzi A. Telencephalic transplants in mice: characterization of growth and differentiation patterns. Neuropathol. Appl. Neurobiol. 22:1996;108-117.
17. Isenmann S., Molthagen M., Brandner S., Bartsch U., Kühne G., Magyar J.P., Sure U., Schachner M., Aguzzi A. The AMOG/β2 subunit of Na,K-ATPase is not necessary for long-term survival of telencephalic grafts. Glia. 15:1995;377-388.
18. Linden J., Taylor H.E., Feldman M.D., Woodward E.B., Ayers C.R., Ripley M.L., Iflah S., Patel A. The precise radioimmunoassay of adenosine: minimization of sample collection artifacts and immunocrossreactivity. Anal. Biochem. 201:1992;246-254.
19. Lindvall O. Neural transplantation in Parkinson's disease. Novartis Found. Symp. 231:2000;110-123.
20. Lindvall O., Bjorklund A. First step towards cell therapy for Huntington's disease. Lancet. 356:2000;1945-1946.
21. Ojemann G.A. Treatment of temporal lobe epilepsy. Annu. Rev. Med. 48:1997;317-328.
22. Poon A., Sawynok J. Antinociception by adenosine analogs and inhibitors of adenosine metabolism in an inflammatory thermal hyperalgesia model in the rat. Pain. 74:1998;235-245.
23. Sweeney M.I. Neuroprotective effects of adenosine in cerebral ischemia: window of opportunity. Neurosci. Biobehav. Rev. 21:1997;207-217.
24. Tovar K.R., Sprouffske K., Westbrook G.L. Fast NMDA receptor-mediated synaptic currents in neurons from mice lacking the epsilon2 (NR2B) subunit. J. Neurophysiol. 83:2000;616-620.