Detection of reactive oxygen species by flow cytometry after spinal cord injury

Journal of Neuroscience Methods

Volumn 120, Issue 1, 2002, Pages 105-112

  Luo, Jian ^a   Li, Nianyu ^a   Paul Robinson, J ^a   Shi, Riyi ^a  

^a PURDUE UNIVERSITY   (United States)

Author keywords

Flow cytometry; Formaldehyde; Lipid peroxidation; Reactive oxygen species; Scavenger; Spinal cord injury

Indexed keywords

FORMALDEHYDE; MANGANESE DERIVATIVE; REACTIVE OXYGEN METABOLITE; SCAVENGER; SUPEROXIDE;

ACCURACY; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL ISOLATION; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GUINEA PIG; INTERMETHOD COMPARISON; LIPID PEROXIDATION; NONHUMAN; PRIORITY JOURNAL; SPINAL CORD INJURY;

ANIMALS; FLOW CYTOMETRY; GUINEA PIGS; HISTOLOGICAL TECHNIQUES; REACTIVE OXYGEN SPECIES; SPINAL CORD INJURIES;

EID: 0037109228     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(02)00193-0     Document Type: Article

References (19)

1. Bindokas V.P., Jordan J., Lee C.C., Miller R.J. Superoxide production in rat hippocampal neurons: selective imaging with hydroethidine. J. Neurosci. 16:1996;1324-1336.
2. Brewer G.J. Isolation and culture of adult rat hippocampal neurons. J. Neurosci. Meth. 71:1997;143-155.
3. Carter W.O., Narayanan P.K., Robinson J.P. Intracellular hydrogen peroxide and superoxide anion detection in endothelial cells. J. Leukoc. Biol. 55:1994;253-258.
4. Coyle J.T., Puttfarcken P. Oxidative stress, glutamate, and neurodegenerative disease. Science. 262:1993;689-695.
5. de Zwart L.L., Meerman J.H., Commandeur J.H., Vermeulen N.P. Biomarkers of free radical damage applications in experimental animals and in humans. Free Radic. Biol. Med. 26:1999;202-226.
6. Diamond R.A., Wang H., Chen F., Wilke-Douglas M. Cell preparation: an enrichment for FCM analysis and cell sorting. Diamond R.A., DeMaggio S., In Living Color: Protocols in Flow Cytometry and Cell Sorting. 2000;111-141 Springer, New York.
7. Gibellini D., Zauli G., Re C.M., Furlini G., Lolli S., Bassini A., Celeghini C., La Placa M. In situ polymerase chain reaction technique revealed by flow cytometry as a tool for gene detection. Anal. Biochem. 228:1995;252-258.
8. Grogan W.M., Collins J.M. Guide to Flow Cytometry Methods. 1990;22-28 Marcel Dekker, New York.
9. Hall E.D. Free radicals and CNS injury. Crit. Care Clin. 5:1989;793-805.
10. Hall E.D. Inhibition of lipid peroxidation in central nervous system trauma and ischemia. J. Neurol. Sci. 134:1995;79-83.
11. Kay A.R., Wong R.K. Isolation of neurons suitable for patch-clamping from adult mammalian central nervous systems. J. Neurosci. Meth. 16:1986;227-238.
12. Larsen J.K., Munch-Petersen B., Christiansen J., Jorgensen K. Flow cytometric discrimination of mitotic cells: resolution of M, as well as G1, S, and G2 phase nuclei with mithramycin, propidium iodide, and ethidium bromide after fixation with formaldehyde. Cytometry. 7:1986;54-63.
13. Ostrovidov S., Franck P., Joseph D., Martarello L., Kirsch G., Belleville F., Nabet P., Dousset B. Screening of new antioxidant molecules using flow cytometry. J. Med. Chem. 43:2000;1762-1769.
14. Pretlow TG, Pretlow TP. Sedimentation for separation of cells. In: Diamond RA, Rothenberg ED, editors. Methods: A Companion to Methods in Enzymology. San Diego, USA: Academic Press, 1991;2(3):184-5.
15. Robinson J.P. Handbook of Flow Cytometry Methods. 1993;Wiley-Liss, New York.
16. Rothe G., Valet G. Flow cytometric analysis of respiratory burst activity in phagocytes with hydroethidine and 2′,7′-dichlorofluorescein. J. Leukoc. Biol. 47:1990;440-448.
17. Valgimigli L., Pedulli G.F., Paolini M. Measurement of oxidative stress by EPR radical-probe technique. Free Radic. Biol. Med. 31:2001;708-716.
18. Visscher D.W., Crissman J.D. Dissociation of intact cells from tumors and normal tissues. Meth. Cell. Biol. 41:1994;1-13.
19. Watson J.V. Introduction to Flow Cytometry. 1991;125-127 Cambridge University Press, Cambridge.