Translation initiation at non-AUG codons mediated by weakened association of eukaryotic initiation factor (eIF) 2 subunits

Biochemical Journal

Volumn 367, Issue 2, 2002, Pages 359-368

  Hashimoto, Nilce N ^a   Carnevalli, Larissa S ^a   Castilho, Beatriz A ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

eIF2 mutations; Non AUG recognition; Subunit interaction determinants; Translation initiation

Indexed keywords

AMINO ACIDS; BIOASSAY; HYDROLYSIS; RNA; SUBSTITUTION REACTIONS; YEAST;

TRANSLATION INITIATION;

BIOCHEMISTRY;

ALANINE; AMINO ACID DERIVATIVE; GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANOSINE TRIPHOSPHATE; HISTIDINE; INITIATION FACTOR 2; INITIATION FACTOR 2 GAMMA; MESSENGER RNA; METHIONINE TRANSFER RNA; MUTANT PROTEIN; PROTEIN SUBUNIT; RIBOSOME PROTEIN; SERINE; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CODON USAGE; GENETIC ASSOCIATION; HYDROLYSIS; IMMUNOPRECIPITATION; LETHAL MUTANT; MOLECULAR INTERACTION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DEFECT; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; RECESSIVE INHERITANCE; RNA BINDING; START CODON; TEMPERATURE SENSITIVITY; TRANSLATION INITIATION; TWO HYBRID SYSTEM; YEAST;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARCHAEAL PROTEINS; BASE SEQUENCE; BINDING SITES; CODON, INITIATOR; CONSERVED SEQUENCE; EUKARYOTIC INITIATION FACTOR-2; GENES, SUPPRESSOR; GUANOSINE TRIPHOSPHATE; HYDROLYSIS; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN BIOSYNTHESIS; PROTEIN SUBUNITS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

ANIMALIA; EUKARYOTA;

EID: 0037109034     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20020556     Document Type: Article

References (24)

1. Hershey, J. W. B. and Merrick, W. C. (2000) Pathway and mechanism of initiation of protein synthesis. In Translational Control of Gene Expression (Sonenberg, N., Hershey, J. W. B. and Mathews, M. B., eds.), pp. 33-88, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
2. Hinnebusch, A. G. (2000) Mechanism and regulation of initiator methionyl-tRNA binding to ribosomes. In Translational Control of Gene Expression (Sonenberg, N., Hershey, J. W. B. and Mathews, M. B., eds.), pp. 185-243, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
3. Pestova, T. V., Lomakin, I. B., Lee, J. H., Choi, S. K., Dever, T. E. and Hellen, C. U. T. (2000) The joining of ribosomal subunits in eukaryotes requires eIF5B. Nature (London) 403, 332-335
4. Donahue, T. F., Cigan, A. M., Pabich, E. K. and Castilho-Valavicius, B. (1988) Mutations at a Zr(II) finger motif in the yeast eIF-2β gene alter ribosomal start-site selection during the scanning process. Cell (Cambridge, Mass.) 54, 621-632
5. Cigan, A. M., Pabich, E. K., Feng, L. and Donahue, T. F. (1989) Yeast translation initiation suppressor sui2 encodes the α subunit of eukaryotic initiation factor 2 and shares sequence identity with the human α subunit. Proc. Natl. Acad. Sci. U.S.A. 86, 2784-2788
6. Castilho-Valavicius, B., Yoon, H. and Donahue, T. F. (1990) Genetic characterization of the Saccharomyces cerevisiae translational initiation suppressors sui1, sui2 and SUI3 and their effects on HIS4 expression. Genetics 124, 483-495
7. Dorris, D. R., Erickson, F. L. and Hannig, E. M. (1995) Mutations in GCDII, the structural gene for eIF-2γ in yeast, alter translational regulation of GCN4 and the selection of the start site for protein synthesis. EMBO J. 14, 2239-2249
8. Huang, H., Yoon, H., Hannig, E. M. and Donahue, T. F. (1997) GTP hydrolysis controls stringent selection of the AUG start codon during translation initiation in Saccharomyces cerevisiae. Genes Dev. 11, 2396-2413
9. Erickson, F. L., Nika, J., Rippel, S. and Hannig, E. M. (2001) Minimum requirements for the function of eukaryotic translation initiation factor 2. Genetics 158, 123-132
10. Erickson, F. L. and Hannig, E. M. (1996) Ligand interactions with eukaryotic translation initiation factor 2: Role of the γ-subunit. EMBO J. 15, 6311-6320
11. Bommer, J. A., Kraft, R., Kurzchalia, T. V., Price, N. T. and Proud, C. G. (1991) Amino acid sequence analysis of the β- and γ-subunits of eukaryotic initiation factor eIF-2. Identification of regions interacting with GTP. Biochim. Biophys. Acta 1079, 308-315
12. Gaspar, N. J., Kinzy, T. G., Scherer, B. J., Humbelin, M., Hershey, J. W. and Merrick, W. C. (1994) Translation initiation factor eIF-2. Cloning and expression of the human cDNA encoding the γ-subunit. J. Biol. Chem. 269, 3415-3422
13. Asano, K., Krishnamoorthy, T., Phan, L., Pavitt, G. D. and Hinnebusch, A. G. (1999) Conserved bipartite motifs in yeast eIF5 and eIF2Bε, GTPase-activating and GDP-GTP exchange factors in translation initiation, mediate binding to their common substrate eIF2. EMBO J. 18, 1673-1688
14. 2 motif. Mol. Cell. Biol. 19, 173-181
15. 2-Cys motif on the β subunit of eukaryotic translation initiation factor 2. Gene Expression 2, 297-309
16. Kimball, S. R., Heinzinger, N. K., Horetsky, R. L. and Jefferson, L. S. (1998) Identification of interprotein interactions between the subunits of eukaryotic initiation factors eIF2 and eIF2B. J. Biol. Chem. 273, 3039-3044
17. Thompson, G. M., Pacheco, E., Melo, E. O. and Castilho, B. A. (2000) Conserved sequences in the β subunit of archaeal and eukaryal translation initiation factor 2 (eIF2), absent from eIF5, mediate interaction with eIF2γ. Biochem. J. 347, 703-709
18. Das, S., Ghosh, R. and Maitra, U. (2001) Eukaryotic translation initiation factor 5 functions as a GTPase-activating protein. J. Biol. Chem. 276, 6720-6726
19. Paulin, F. E., Campbell, L. E., O'Brien, K., Loughlin, J. and Proud, C. G. (2001) Eukaryotic translation initiation factor 5 (eIF5) acts as a classical GTPase-activator protein. Curr. Biol. 11, 55-59
20. Guthrie, C. and Fink, G. R. (1991) Guide to Yeast Genetics and Molecular Biology, Methods in Enzymology, vol. 194, Academic Press, San Diego
21. James, P., Halladay, J. and Craig, E. A. (1996) Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast Genetics 144, 1425-1436
22. Smith, D. B. and Johnson, K. (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40
23. Bult, C. J., White, O., Olsen, G. J., Zhou, L., Fleischmann, R. D., Sutton, G. G., Blake, J. A., FitzGerald, L. M., Clayton, R. A., Gocayne, J. D. et al. (1996) Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science (Washington, D.C.) 273, 1058-1073
24. Das, S., Maiti, T., Das, K. and Maitra, U. (1997) Specific interaction of eukaryotic translation initiation factor 5 (eIF5) with the β-subunit of eIF2. J. Biol. Chem. 272, 31712-31718