Importance of product inhibition in the kinetics of the acylase hydrolysis reaction by differential stopped flow microcalorimetry

Analytical Biochemistry

Volumn 308, Issue 2, 2002, Pages 285-293

  Stödeman, Magnus ^a   Schwarz, Frederick P ^a  

^a NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

Author keywords

Enzyme kinetics; N acetyl L amino acids; Porcine kidney acylase I; Reaction enthalpies; Stopped flow microcalorimetry

Indexed keywords

AMINO ACIDS; CALORIMETERS; ENTHALPY; ENZYME KINETICS; HYDROLYSIS; MIXTURES;

ACYLASE I; CATALYTIC CONSTANTS; ISOTHERMAL TITRATION CALORIMETRY; MICHAELIS-MENTEN EQUATIONS; N-ACETYL-L-AMINO ACIDS; REACTION ENTHALPIES; STOPPED FLOW; SUBSTRATE CONCENTRATIONS;

REACTION KINETICS;

ALANINE DERIVATIVE; AMIDASE; GLYCINE DERIVATIVE; N ACETYLMETHIONINE; PHENYLALANINE DERIVATIVE;

ARTICLE; CHEMICAL REACTION KINETICS; ENTHALPY; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; MICHAELIS CONSTANT; MICROCALORIMETRY; NEGATIVE FEEDBACK; PRIORITY JOURNAL;

SUIDAE;

EID: 0037108837     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00339-1     Document Type: Article

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