A high-throughput microplate method for assessing aggregation of deoxygenated hemoglobin S heterotetramers in vitro

Analytical Biochemistry

Volumn 306, Issue 2, 2002, Pages 349-352

  He, Zhenning ^a,b   Russell, J Eric ^a,b  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b CHILDREN S HOSPITAL OF PHILADELPHIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

HETEROTETRAMERS; HIGH THROUGHPUT; IN-VITRO; MICROPLATES;

CHEMISTRY;

HEMOGLOBIN S; TETRAMER; OXYGEN;

ANALYTIC METHOD; ARTICLE; HEMOGLOBIN DETERMINATION; HUMAN; OSMOLALITY; OXYGENATION; PH; POLYMERIZATION; PRIORITY JOURNAL; REPRODUCIBILITY; TEMPERATURE DEPENDENCE; CHEMISTRY; METHODOLOGY; PROTEIN QUATERNARY STRUCTURE; SPECTROPHOTOMETRY; THERMODYNAMICS;

HEMOGLOBIN, SICKLE; OXYGEN; PROTEIN STRUCTURE, QUATERNARY; SPECTROPHOTOMETRY; THERMODYNAMICS;

EID: 0037098945     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2002.5683     Document Type: Article

References (10)

1. Crystal structure of sickle-cell deoxyhemoglobin at 5 Å resolution
2. The high-resolution crystal structure of deoxyhemoglobin S
3. Supersaturation in sickle cell hemoglobin solutions
4. The solubility of sickle and non-sickle hemoglobins in a concentrated phosphate buffer
5. Role of hydrophobicity of phenylhydrazine beta 85 and leucine beta 88 in the acceptor pocket for valine beta 6 during hemoglobin S polymerization
6. Recombinant human hemoglobins designed for gene therapy of sickle cell disease
7. Hereditary persistence of foetal hemoglobin with beta-chain synthesis in cis position (Gγ-β(+)-HPFH) in a Negro family
8. 2) assembled in complex transgenic-knockout mice
9. Preparation and properties of apohemoglobin and reconstituted hemoglobins
10. Nucleation-controlled aggregation of deoxyhemoglobin S. I. Possible difference in the size of nuclei in different phosphate concentrations