Bacterial expression of in vivo-biotinylated aequorin for direct application to bioluminometric hybridization assays

Analytical Biochemistry

Volumn 306, Issue 2, 2002, Pages 314-322

  Verhaegen, Monique ^a   Christopoulos, Theodore K ^b  

^a UNIVERSITY OF WINDSOR   (Canada)

^b UNIVERSITY OF PATRAS   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

CROSSLINKING; DNA; GENES; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION;

CHEMICAL CROSS-LINKING; COEFFICIENT OF VARIATION; HYBRIDIZATION ASSAY; MONOMERIC AVIDINS; OLIGONUCLEOTIDE PROBES; PHOTOPROTEIN AEQUORIN; REPORTER MOLECULES; SIGNAL-TO-BACKGROUND RATIO;

COENZYMES;

AEQUORIN; AVIDIN; BIOTIN; CALCIUM; LIGASE; PHOTOPROTEIN; STREPTAVIDIN; BACTERIAL PROTEIN; BIRA PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; REPRESSOR PROTEIN; TRANSCRIPTION FACTOR;

ANALYTIC METHOD; ARTICLE; BACTERIUM; BIOLUMINESCENCE; BIOTINYLATION; CELL LYSATE; COMPLEX FORMATION; CROSS LINKING; DNA DENATURATION; HYBRIDIZATION; NONHUMAN; OLIGONUCLEOTIDE PROBE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PROCESSING; GENETICS; ISOLATION AND PURIFICATION; LUMINESCENCE; MOLECULAR CLONING; NUCLEIC ACID HYBRIDIZATION; STAINING;

BACTERIA (MICROORGANISMS);

AEQUORIN; BACTERIAL PROTEINS; BIOTINYLATION; CARBON-NITROGEN LIGASES; CLONING, MOLECULAR; ESCHERICHIA COLI PROTEINS; LUMINESCENT MEASUREMENTS; NUCLEIC ACID HYBRIDIZATION; PLASMIDS; REPRESSOR PROTEINS; STAINING AND LABELING; TRANSCRIPTION FACTORS;

EID: 0037098939     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2002.5724     Document Type: Article

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