A novel ultraviolet assay for testing side reactions of carbodiimides

Analytical Biochemistry

Volumn 305, Issue 2, 2002, Pages 135-138

  Wrobel, Nadia ^a   Schinkinger, Manfred ^b   Mirsky, Vladimir M ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b SY LAB Gerate GmbH   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL BONDS; HYDROLYSIS; PEPTIDES; SYNTHESIS (CHEMICAL);

BIOLOGICAL MOLECULE; BIOLOGICAL SAMPLES; EXTINCTION COEFFICIENTS; MEDIATED REACTIONS; ORGANIC CHEMISTRY; PEPTIDE BOND FORMATION; PEPTIDE SYNTHESIS; ULTRAVIOLET REGION;

CROSSLINKING;

1 (3 DIMETHYLAMINOPROPYL) 3 ETHYLCARBODIIMIDE; ACETIC ACID; BUFFER; CITRIC ACID; CYANAMIDE; PHOSPHATE; CARBOXYLIC ACID;

ARTICLE; CHEMICAL BOND; CONCENTRATION RESPONSE; CROSS LINKING; HYDROLYSIS; PEPTIDE SYNTHESIS; PH; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; SPECTRAL SENSITIVITY; TEMPERATURE; ULTRAVIOLET SPECTROPHOTOMETRY; CHEMISTRY; KINETICS; METABOLISM;

BUFFERS; CARBOXYLIC ACIDS; ETHYLDIMETHYLAMINOPROPYL CARBODIIMIDE; HYDROGEN-ION CONCENTRATION; KINETICS; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 0037096065     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2002.5646     Document Type: Article

References (11)

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7. The carbodiimide method
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9. Aktivierung mit carbodiimiden
10. A new mechanism involving cyclic tautomers for the reaction with nucleophiles of the water-soluble peptide coupling reagent 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide (EDC)