Dual labeling of the fibronectin matrix and actin cytoskeleton with green fluorescent protein variants

Journal of Cell Science

Volumn 115, Issue 6, 2002, Pages 1221-1229

  Ohashi, Tomoo ^a   Kiehart, Daniel P ^a   Erickson, Harold P ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Actin; Fibronectin; Green fluorescent protein; Matrix assembly; Moesin

Indexed keywords

ACTIN; CYTOCHALASIN; FIBRONECTIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MOESIN;

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; CELL CULTURE; CELL LABELING; CELL STRAIN 3T3; CELL SURFACE; CONTROLLED STUDY; CYTOSKELETON; EXTRACELLULAR MATRIX; LAMELLAR BODY; MOLECULAR INTERACTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING;

3T3 CELLS; ACTINS; ANIMALS; CELL MOVEMENT; CELLS, CULTURED; CYTOCHALASINS; EXTRACELLULAR MATRIX; FIBRONECTINS; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; INDICATORS AND REAGENTS; INTEGRINS; LUMINESCENT PROTEINS; MICE; MICROFILAMENT PROTEINS; MICROFILAMENTS; RECOMBINANT FUSION PROTEINS;

ANIMALIA; PUNCTUM MINUTISSIMUM;

EID: 0037087773     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. Actin dynamics in living mammalian cells
2. Self-assembly of fibronectin into fibrillar networks underneath dipalmitoyl phosphatidylcholine monolayers: Role of lipid matrix and tensile forces
3. Zipper nonmuscle myosin-II functions downstream of PS2 integrin in Drosophila myogenesis and is necessary for myofibril formation
4. Preparation of orientated fibrous mats from fibronectin: Composition and stability
5. Integrins and actin filaments: Rerciprocal regulation of cell adhesion and signaling
6. GFP-moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila
7. Production of artificial-orientated mats and strands from plasma fibronectin: A morphological study
8. Dual-colour imaging with GFP variants
9. Defects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin
10. Mechanical properties of the extracellular matrix influence fibronectin fibril assembly in vitro
11. Relationships between fibronectin (LETS protein) and actin
12. Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila
13. Imaging of dynamic changes of the actin cytoskeleton in microextensions of live NIH3T3 cells with a GFP fusion of the F-actin binding domain of Moesin
14. Assembly of fibronectin into extracellular matrix
15. In vitro formation of disulfide-bonded fibronectin multimers
16. Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin-green fluorescent protein
17. Integrin dynamics and matrix assembly: Tensin-dependent translocation of alpha(5)beta(1) integrins promotes early fibronectin fibrillogenesis
18. Conformation of fibronectin fibrils varies: Discrete globular domains of type III repeats detected
19. Plasma fibronectin supports neuronal survival and reduces brain injury following transient focal cerebral ischemia but is not essential for skin-wound healing and hemostasis
20. A novel RGD-independent fibronectin assembly pathway initiated by alpha 4 beta 1 integrin binding to the alternatively spliced V region
21. The fibronexus: A transmembrane association of fibronectin-containing fibers and bundles of 5 nm microfilaments in hamster and human fibroblasts
22. Focal adhesion motility revealed in stationary fibroblasts
23. Microfilament dynamics during cell movement and chemotaxis monitored using a GFP-actin fusion protein
24. Integrin activation and cytoskeletal interaction are essential for the assembly of a fibronectin matrix
25. Rho-mediated contractility exposes a cryptic site in fibronectin and induces fibronectin matrix assembly