A photochemical/chemical direct method of synthesizing high-performance deoxyribonucleic acid chips for rapid and parallel gene analysis

Sensors and Actuators, B: Chemical

Volumn 83, Issue 1-3, 2002, Pages 67-76

  Takahashii, Kazunori ^a   Seio, Kohji ^b   Sekine, Mitsuo ^b   Hino, Okio ^c   Esashi, Masayoshi ^d  

^a UNIVERSITY OF TOKYO   (Japan)

^b TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^c CANCER INSTITUTE   (Japan)

^d TOHOKU UNIVERSITY   (Japan)

Author keywords

DNA chip; Photochemical synthesis; Photolithography; Photosensitive reagent; Polymorphism; SNP analysis

Indexed keywords

ARRAYS; GENETIC ENGINEERING; MUTAGENESIS; PHOTOCHEMICAL REACTIONS; PHOTOLITHOGRAPHY; PHOTOSENSITIVITY; SYNTHESIS (CHEMICAL);

PHOTOCHEMICAL SYNTHESIS; POLYMORPHISM;

DNA;

EID: 0037086025     PISSN: 09254005     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0925-4005(01)01030-9     Document Type: Conference Paper

References (10)

1. Light-generated oligonucleotide arrays for rapid DNA sequence analysis
2. Microarray fabrication with covalent attachment of DNA using bubble jet technology
3. Combining the preparation of oligonucleotide arrays and synthesis of high-quality primers
4. Covalent attachment of synthetic DNA to self-assembled monolayer films
5. Photolabile protecting groups for nucleosides: Synthesis and photodeprotection rates
6. The final deprotection step in oligonucleotide synthesis is reduced to a mild and rapid ammonia treatment by using labile base-protecting groups
7. DNA sequencing on a chip
8. Proofing of photolithographic DNA synthesis with 3′,5′-dimethoxy-benzoinyloxy-carbonyl-protected deoxynucleoside phosphoramidites
9. High-density synthetic oligonucleotide array