An ELISA technique for quantification of surfactant apoprotein (SP)-C in bronchoalveolar lavage fluid

American Journal of Respiratory and Critical Care Medicine

Volumn 165, Issue 4, 2002, Pages 470-474

  Schmidt, Reinhold ^a   Steinhilber, Wolfram ^b   Ruppert, Clemens ^a   Daum, Christina ^a   Grimminger, Friedrich ^a   Seeger, Werner ^a   Günther, Andreas ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

^b ALTANA PHARMA AG   (Germany)

Author keywords

ARDS; Bronchoalveolar lavage fluid; ELISA; Surfactant

Indexed keywords

2 PROPANOL; METHANOL; PEPTIDE; PHOSPHOLIPID; POLYCLONAL ANTIBODY; POLYSTYRENE; SURFACTANT PROTEIN C; TRIFLUOROETHANOL;

ANTIGEN PRESENTATION; ARTICLE; CROSS REACTION; DILUTION; DISEASE ASSOCIATION; ENZYME LINKED IMMUNOSORBENT ASSAY; HYDROPHOBICITY; LUNG ALVEOLUS SURFACE TENSION; LUNG LAVAGE; MICROTITER PLATE ASSAY; PH; PRIORITY JOURNAL; PROTEIN BINDING; REPRODUCIBILITY; RESPIRATORY DISTRESS SYNDROME;

EID: 0037084031     PISSN: 1073449X     EISSN: None     Source Type: Journal    
DOI: 10.1164/ajrccm.165.4.2102080     Document Type: Article

References (31)

1. Pulmonary surfactant and its apoprotelns
2. Molecular structures and interactions of pulmonary surfactant components
3. Surfactant protein C: A review of its unique properties and metabolism
4. Structure and properties of surfactant protein C
5. Surfactant-associated proteins. Functions and structural variation
6. Biophysical activity of synthetic phospholipids combined with purified lung surfactant 6000 dalton apoprotein
7. Role of bovine pulmonary surfactant-associated proteins in the surface-active property of phospholipid mixtures
8. Evidence of lung surfactant abnormality in respiratory, failure
9. Surfactant chemical composition and biophysical activity in acute respiratory distress syndrome
10. Surfactant alterations in severe pneumonia, acute respiratory distress syndrome, and cardiogenic lung edema
11. Surfactant inhibition by plasma proteins: Differential sensitivity of various surfactant preparations
12. Lung surfactant phospholipids associate with polymerizing fibrin-loss of surfactant activity
13. Surfactant subtype conversion is related to loss of surfactant apoprotein B and surface activity in the large surfactant aggregates - Experimental and clinical studies
14. Suffactant protein A (SP-A) is decreased in acute parenchymal lung injury associated with polytrauma
15. Quantitative analysis of pulmonary surfactant proteins B and C
16. Purification of a hydrophobic surfactant peptide using high-performance liquid chromatography
17. ε-palmitoyl-lysine
18. A quantitative method for detecting surfactant-associated protein C in pulmonary surfactant
19. Surfactant protein composition of lamellar bodies isolated from rat lung
20. ELISA technique for quantification of surfactant protein B (SP-B) in bronchoalveolar lavage fluid
21. Surfactant protein C in fetal and ventilated preterrn rabbit lungs
22. A rapid method of total lipid extraction and purification
23. Low molecular weight human pulmonary surfactant protein (SP5): Isolation, characterization, and cDNA and amino acid sequences
24. Tricine-sodium-dodecylsulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
25. Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity
26. The American-European consensus conference on ARDS. Definitions, mechanisms, relevant outcomes, and clinical trial coordination
27. Two-dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots
28. The acylation of glycerophosphate in rat liver. A new assay procedure for glycerophosphate acylation, studies on its subcellular and submitochondrial localization and determination of the reaction products
29. Pulmonary surfactant-associated polypeptide C in a mixed organic solvent transforms from a monomeric alpha-helical state into insoluble beta-sheet aggregates
30. An antibody with specificity for surfactant protein C precursors: Identification of Pro-SP-C in rat lung