Selective determination of mitochondrial chelatable iron in viable cells with a new fluorescent sensor

Biochemical Journal

Volumn 362, Issue 1, 2002, Pages 137-147

  Petrat, Frank ^a   Weisheit, Daniela ^a   Lensen, Martina ^a   De Groot, Herbert ^a   Sustmann, Reiner ^a   Rauen, Ursula ^a  

^a UNIVERSITY HOSPITAL ESSEN   (Germany)

Author keywords

Chelators; Fluorescence; Indicators; Mitochondria

Indexed keywords

BIOSENSORS; CHELATION; DISEASES; ESTERS; FLUORESCENCE; IRON; QUENCHING; STOICHIOMETRY; TRANSITION METALS;

FLUORESCENT SENSORS; VIABLE CELLS;

CELL CULTURE;

FLUORESCENT DYE; IRON; RHODAMINE B [(1,10 PHENANTHROLIN 5 YL)AMINOCARBONYL]BENZYL ESTER; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL LEVEL; CONTROLLED STUDY; FLUORESCENCE; MALE; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; RAT; STOICHIOMETRY;

ANIMALS; CELLS, CULTURED; FLUORESCENT DYES; HEME; IRON; IRON CHELATING AGENTS; MALE; MITOCHONDRIA, LIVER; RATS; RATS, WISTAR; RHODAMINES; SPECTROMETRY, FLUORESCENCE;

ANIMALIA;

EID: 0037083893     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3620137     Document Type: Article

References (44)

1. Increase in bleomycin-detectable iron in ischaemia/reperfusion injury to rat kidneys
2. Iron loading of endothelial cells augments oxidant damage
3. Nutrient deprivation of cultured rat hepatocytes increases the desferrioxamine-available iron pool and augments the sensitivity to hydrogen peroxide
4. Increase in cellular pool of low-molecular-weight iron during ethanol metabolism in rat hepatocyte cultures
5. Low molecular weight iron and the oxygen paradox in isolated rat hearts
6. 2 formation
7. Cobalt chloride and desferrioxamine antagonize the inhibition of erythropoietin production by reactive oxygen species
8. Iron acquired from transferrin by K562 cells is delivered into a cytoplasmic pool of chelatable iron(II)
9. Fluorescence analysis of the labile iron pool of mammalian cells
10. The liver and iron
11. Friedreich ataxia: An overview
12. Recent advances in the molecular pathogenesis of Friedreich ataxia
13. Mitochondrial dysfunction in neurodegenerative diseases
14. Oxidative stress and Alzheimer disease
15. Fragile mitochondrial DNA: The missing link in the apoptotic neuronal cell death in Parkinson's disease
16. Mitochondrial dysfunction in neurodegenerative disorders
17. Relationship between free iron level and rat liver mitochondrial dysfunction in experimental dietary iron overload
18. Mitochondrial 'non-heme non FeS iron' and its significance in the cellular metabolism of iron
19. Mitochondrial iron not bound in heme and iron-sulfur centers-estimation, compartmentation and redox state
20. Subcellular distribution of chelatable iron: A laser scanning microscopic study in isolated rat hepatocytes and rat liver endothelial cells
21. The improved synthesis of 5-nitro-1,10-phenanthroline
22. Synthesis and characterisation of a new DNA-binding bifunctional ruthenium(II) complex
23. An efficient synthesis of N-α-Fmoc-4-(Phosphonodifluoromethyl)-L-phenylalanine
24. Determination of the chelatable iron pool of single intact cells by laser scanning microscopy
25. + in individual fibroblasts and lymphocytes
26. Modulation of the mitochondrial permeability transition pore by pyridine nucleotides and dithiol oxidation at two separate sites
27. In vitro oxidation of vitamin E, vitamin C, thiols and cholesterol in rat brain mitochondria incubated with free radicals
28. Measurement of iron and copper in biological systems: Bleomycin and copper-phenanthroline assays
29. 2 liberated by sources other than xanthine oxidase
30. Determination of the chelatable iron pool of isolated rat hepatocytes by digital fluorescence microscopy using the fluorescent probe, phen green SK
31. Membrane potential can be determined in individual cells from the Nernstian distribution of cationic dyes
32. Iron mobilization from myocardial cells by 3-hydroxypyridin-4-one chelators: Studies in rat heart cells in culture
33. The reticulocyte plasma membrane pathway of iron uptake as determined by the mechanism of a α,α'-dipyridyl inhibition
34. Iron mobilization from hepatocyte monolayer cultures by chelators: The importance of membrane permeability and the iron-binding constant
35. Tissue-specific regulation of iron metabolism and heme synthesis: Distinct control mechanisms in erythroid cells
36. 59Fe
37. Iron and copper transport in yeast and its relevance to human disease
38. Ferrochelatase, glutathione peroxidase and transferrin receptor mRNA synthesis and levels in mouse erythroleukemia cells
39. Are reactive oxygen species involved in Alzheimer's disease?
40. Oxidant mechanisms in toxic acute renal failure
41. Oxidant mechanisms in gentamicin nephrotoxicity
42. Molecular biology of aging