Comparison of preparative and analytical two-dimensional electrophoresis for isolation and matrix-assisted laser desorption/ionization - Time-of-flight mass spectrometric analysis of transthyretin in cerebrospinal fluid

Analytical Biochemistry

Volumn 301, Issue 2, 2002, Pages 161-167

  Westman Brinkmalm, Ann ^a   Davidsson, Pia ^a  

^a SAHLGRENSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Analytical 2 DE; Cerebrospinal fluid; Liquid phase IEF; Mass spectrometry; Prefractionation; Preparative 2 DE; Transthyretin

Indexed keywords

BRAIN; DESORPTION; ELECTROPHORESIS; LIQUIDS; MASS SPECTROMETERS; MASS SPECTROMETRY; PROTEINS;

ANALYTICAL 2-DE; LIQUID PHASE; PREFRACTIONATION; PREPARATIVE 2-DE; TRANSTHYRETIN;

CEREBROSPINAL FLUID;

PREALBUMIN;

AMINO ACID SEQUENCE; ARTICLE; DRUG CEREBROSPINAL FLUID LEVEL; DRUG ISOLATION; HUMAN; INTERMETHOD COMPARISON; ISOELECTRIC FOCUSING; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR BIOLOGY; MOLECULAR MODEL; PEPTIDE ANALYSIS; PHASE SEPARATION; PRIORITY JOURNAL; PROTEIN PROCESSING; QUANTITATIVE ASSAY; TWO DIMENSIONAL ELECTROPHORESIS;

EID: 0037083351     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5487     Document Type: Article

References (12)

1. Identification of synaptic vesicle, pre- and postsynaptic proteins in human cerebrospinal fluid using liquid phase isoelectric focusing
2. Characterization of proteins from human cerebrospinal fluid by a combination of preparative two-dimensional liquid phase electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
3. Peptide mapping of proteins in cerebrospinal fluid utilizing a rapid preparative two-dimensional electrophoretic procedure and matrix-assisted laser desorption/ionization mass spectrometry
4. Analysis of intact proteins from cerebrospinal fluid by matrix-assisted laser desorption/ionization mass spectrometry after two-dimensional liquid-phase electrophoresis
5. Identification of proteins in pleural exudate using preparative two-dimensional gel electrophoresis and MALDI-TOF masspectrometry
6. Detection of different forms of variant transthyretin (Met30) in cerebrospinal fluid
7. Characterization of transthyretin mutants from serum using immunoprecipitation, HPLC/electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry
8. A rapid method to characterize mutations transthyretin in cerebrospinal fluid from familial amyloidotic polyneuropathy patients using matrix-assisted laser desorption/ionization mass spectrometry
9. Preparation and solubilization of body fluids for 2-D
10. Mass spectrometric sequencing of proteins from silverstained polyacrylamide gels
11. Comparison of MALDI-MS sample preparation strategies for proteins in complex biological mixtures