RT-PCR analysis of 5′ to 3′-end-ligated mRNAs identifies the extremities of cox2 transcripts in pea mitochondria

Nucleic Acids Research

Volumn 30, Issue 2, 2002, Pages 439-446

  Kuhn, Josef ^a   Binder, Stefan ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; CYTOCHROME C OXIDASE; MESSENGER RNA; MITOCHONDRIAL RNA; NUCLEASE S1; NUCLEOTIDE; THYMIDINE;

ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; IN VITRO STUDY; NONHUMAN; NUCLEIC ACID ANALYSIS; NUCLEOTIDE SEQUENCE; PEA; PRIORITY JOURNAL; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA PROCESSING; RNA TRANSCRIPTION; TRANSCRIPTION INITIATION; VALIDATION PROCESS;

EMBRYOPHYTA; PISUM SATIVUM;

EID: 0037079666     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/30.2.439     Document Type: Article

References (30)

1. The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366,924 nucleotides
2. Cys (GCA)
3. The RNA world of plant mitochondria
4. A conserved 11 nucleotide sequence contains an essential promoter element of the maize mitochondrial atp1 gene
5. Architecture of the maize mitochondrial atp1 promoter as determined by linker-scanning and point mutagenesis
6. A conserved core element is functionally important for maize mitochondrial promoter activity in vitro
7. A novel pea mitochondrial in vitro transcription system recognizes homologous and heterologous mRNA and tRNA promoters
8. Continuous primary sequence requirements in the 18-nucleotide promoter of dicot plant mitochondria
9. Polyadenylation accelerates the degradation of the mitochondrial mRNA associated with cytoplasmic male sterility in sunflower
10. Polyadenylation occurs at multiple sites in maize mitochondrial cox2 mRNA and is independent of editing status
11. Transcript lifetime is balanced between stabilizing stem-loop structures and degradation-promoting polyadenylation in plant mitochondria
12. Addition of non-genomically encoded nucleotides to the 3′-terminus of maize mitochondrial mRNAs: Truncated rps12 mRNAs frequently terminate with CCA
13. Cys in mitochondria of Oenothera berteriana is initiated in precursor molecules
14. Phe from precursors in plant mitochondria
15. Cotranscription of the rpl5-rps14-cob gene cluster in pea mitochondria
16. Pea cytochrome oxidase subunit II gene has no intron and generates two mRNA transcripts with different 5′-termini
17. Transfer RNA editing in land snail mitochondria
18. Differences in editing at homologous sites in messenger RNAs from angiosperm mitochondria
19. Characterization of transcription initiation sites on the soybean mitochondrial genome allows identification of a transcription-associated sequence motif
20. Asn genes in potato mitochondria: Mapping of transcription initiation sites present in the trnN1-trnY-nad2 cluster and upstream of trnN2
21. Characterization of the potato mitochondrial transcription unit containing 'native' trnS (GCU), trnF (GAA) and trnP (UGG)
22. Transcription of potato mitochondrial 26S rRNA is initiated at its mature 5′ end
23. 3′-Inverted repeats in plant mitochondrial mRNAs are processing signals rather than transcription terminators
24. The steady-state level of mRNA from the Ogura cytoplasmic male sterility locus in Brassica cybrids is determined post-transcriptionally by its 3′ region
25. Prediction of rho-independent transcriptional terminators in Escherichia coli
26. Bacteriophage T4 RNA ligase. Reaction intermediates and interaction of substrates
27. Dinucleoside pyrophosphate are substrates for T4-induced RNA ligase
28. Sequencing the termini of capped viral RNA by 5′-3′ ligation and PCR
29. A highly sensitive method for mapping the 5′ termini of mRNAs